Megahertz serial crystallography [PDF]
Nature Communications, 2018 The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently ...
Max O. Wiedorn +125 more
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Serial crystallography captures enzyme catalysis in copper nitrite reductase at atomic resolution from one crystal [PDF]
IUCrJ, 2016 Relating individual protein crystal structures to an enzyme mechanism remains a major and challenging goal for structural biology. Serial crystallography using multiple crystals has recently been reported in both synchrotron-radiation and X-ray free ...
Sam Horrell +5 more
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Room-temperature X-ray fragment screening with serial crystallography [PDF]
Nature CommunicationsStructural insights into protein-ligand interactions are essential for advancing drug development, with macromolecular X-ray crystallography being a cornerstone technique.
Sebastian Günther +13 more
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Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals [PDF]
IUCrJ, 2020 Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful ...
Alexander M. Wolff +37 more
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Serial crystallography is just getting started [PDF]
Structural DynamicsWhen serial crystallography at XFELs was first being developed, it was unclear how it should best be used. Early experiments included studying single crystals, or weak or small crystals, or sample delivery methods that over used crystal material or under
Aaron S Brewster +3 more
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Exploring serial crystallography for drug discovery [PDF]
IUCrJStructure-based drug design is highly dependent on the availability of structures of the protein of interest in complex with lead compounds. Ideally, this information can be used to guide the chemical optimization of a compound into a pharmaceutical drug
A. Dunge +7 more
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Robust error calibration for serial crystallography [PDF]
Structural DynamicsSerial crystallography is an important technique with unique abilities to resolve enzymatic transition states, minimize radiation damage to sensitive metalloenzymes, and perform de novo structure determination from micron sized crystals.
David W. Mittan-Moreau +6 more
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In situ serial crystallography facilitates 96-well plate structural analysis at low symmetry [PDF]
IUCrJThe advent of serial crystallography has rejuvenated and popularized room-temperature X-ray crystal structure determination. Structures determined at physiological temperature reveal protein flexibility and dynamics. In addition, challenging samples (e.g.
Nicolas Foos +13 more
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Droplet microfluidics for time-resolved serial crystallography [PDF]
IUCrJSerial crystallography requires large numbers of microcrystals and robust strategies to rapidly apply substrates to initiate reactions in time-resolved studies.
Jack Stubbs +13 more
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Data reduction in protein serial crystallography [PDF]
IUCrJSerial crystallography (SX) has become an established technique for protein structure determination, especially when dealing with small or radiation-sensitive crystals and investigating fast or irreversible protein dynamics. The advent of newly developed
Marina Galchenkova +9 more
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