Results 21 to 30 of about 29,886 (251)

Sample Delivery Media for Serial Crystallography [PDF]

International Journal of Molecular Sciences, 2019
X-ray crystallographic methods can be used to visualize macromolecules at high resolution. This provides an understanding of molecular mechanisms and an insight into drug development and rational engineering of enzymes used in the industry. Although conventional synchrotron-based X-ray crystallography remains a powerful tool for understanding molecular
Ki-Hyun Nam
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Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

Nature Communications, 2017
Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for ...
Tobias Weinert, Natacha Olieric, Robert Cheng, Steffen Brünle, Daniel James, Dmitry Ozerov, Dardan Gashi, Laura Vera, May Marsh, Kathrin Jaeger, Florian Dworkowski, Ezequiel Panepucci, Shibom Basu, Petr Skopintsev, Andrew S. Doré, Tian Geng, Robert M. Cooke, Mengning Liang, Andrea E. Prota, Valerie Panneels, Przemyslaw Nogly, Ulrich Ermler, Gebhard Schertler, Michael Hennig, Michel O. Steinmetz, Meitian Wang, Jörg Standfuss   +26 more
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Guide to serial synchrotron crystallography

Current Research in Structural Biology
Serial crystallography (SX) is an emerging technique that can be used to determine the noncryogenic crystal structure of macromolecules while minimizing radiation damage.
Ki Hyun Nam
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High-speed fixed-target serial virus crystallography [PDF]

Nature Methods, 2017
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with
Alonso-Mori, R, Brewster, AS, Damiani, DS, David, C, Duman, R, Fischer, P, Fry, EE, Ginn, HM, Harlos, K, Kelly, J, Kotecha, A, Meents, A, Meyer, J, Michels-Clark, T, Nelson, S, Pakendorf, T, Reime, B, Ren, J, Roedig, P, Rowlands, DJ, Sauter, NK, Sikorsky, M, Stuart, DI, Sutton, G, Vartiainen, I, Wagner, A, Walter, TS, Warmer, M, Young, ID   +28 more
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Serial Crystallography for Structure-Based Drug Discovery [PDF]

Trends in Pharmacological Sciences, 2020
Rational drug discovery has greatly accelerated the development of safer and more efficacious therapeutics, assisted significantly by insights from experimentally determined 3D structures of ligands in complex with their targets. Serial crystallography (SX) with X-ray free-electron lasers has enabled structural determination using micrometer- or ...
Lan, Zhu, Xiaoyu, Chen, Enrique E, Abola, Liang, Jing, Wei, Liu   +4 more
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Serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A brief history is given of how X-ray diffraction data from crystals have been recorded. Today there are new possibilities, spawned by the availability of free electron lasers that produce powerful femtosecond long X-ray pulses.
Michael G. Rossmann
doaj   +3 more sources

X-ray lasers and serial crystallography [PDF]

IUCrJ, 2015
A summary is given of the achievements and opportunities which resulted from the first use of an X-ray laser for serial crystallography and related methods in 2009.
John C. H. Spence
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Serial crystallography using automated drop dispensing [PDF]

Journal of Synchrotron Radiation, 2021
Automated, pulsed liquid-phase sample delivery has the potential to greatly improve the efficiency of both sample and photon use at pulsed X-ray facilities. In this work, an automated drop on demand (DOD) system that accelerates sample exchange for serial femtosecond crystallography (SFX) is demonstrated.
Zhen Su, Joshua Cantlon, Lacey Douthit, Max Wiedorn, Sébastien Boutet, Jan Kern, Chun Hong Yoon, Daniel DePonte   +7 more
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Lipidic cubic phase serial millisecond crystallography using synchrotron radiation. [PDF]

, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins.Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Berntsen, P, Burghammer, M, Båth, P, Chapman, H, Cherezov, V, Heymann, M, Jaeger, K, James, D, Johansson, L, Liu, H, Metz, M, Moraes, I, Nelson, G, Neutze, R, Nogly, P, Oberthuer, D, Panneels, V, Schertler, G, Shilova, A, Spence, J, Standfuss, J, Wang, D, Weierstall, U, White, TA, Wickstrand, C, Wu, W, Zatsepin, N   +26 more
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Nanoflow electrospinning serial femtosecond crystallography [PDF]

Acta Crystallographica Section D Biological Crystallography, 2012
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or ...
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J.   +37 more
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