Results 31 to 40 of about 11,269 (219)

Asymmetry in serial femtosecond crystallography data [PDF]

Acta Crystallographica Section A Foundations and Advances, 2017
Serial crystallography is an increasingly important approach to protein crystallography that exploits both X-ray free-electron laser (XFEL) and synchrotron radiation. Serial crystallography recovers complete X-ray diffraction data by processing and merging diffraction images from thousands of randomly oriented non-uniform microcrystals, of which all ...
Amit Sharma, Linda Johansson, Elin Dunevall, Weixiao Y. Wahlgren, Richard Neutze, Gergely Katona   +5 more
openaire   +3 more sources

Whole-pattern fitting technique in serial femtosecond nanocrystallography

IUCrJ, 2016
Serial femtosecond X-ray crystallography (SFX) has created new opportunities in the field of structural analysis of protein nanocrystals. The intensity and timescale characteristics of the X-ray free-electron laser sources used in SFX experiments ...
Ruben A. Dilanian, Sophie R. Williams, Andrew V. Martin, Victor A. Streltsov, Harry M. Quiney   +4 more
doaj   +1 more source

Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

Droplet streams for serial crystallography of proteins

Experiments in Fluids, 2007
Serial diffraction of proteins requires an injection method to deliver protein molecules - preferably uncharged, fully hydrated, spatially oriented, and with high flux - into the crossed beams of an alignment laser and a focused probe beam of electrons or X-rays of typically only a few tens of microns diameter.
Weierstall, U., Doak, R., Spence, J., Starodub, D., Shapiro, D., Kennedy, P., Warner, J., Hembree, G., Fromme, P., Chapman, H.   +9 more
openaire   +3 more sources

Serial Room Temperature Crystal Loading, Data Collection, and Data Processing of Human Glutaminase C in Complex with Allosteric Inhibitors

Bio-Protocol, 2022
Cancer cells often overexpress glutaminase enzymes, in particular glutaminase C (GAC). GAC resides in the mitochondria and catalyzes the hydrolysis of glutamine to glutamate.
Shawn Milano, D. Szebenyi, Richard Cerione   +2 more
doaj   +1 more source

Serial Femtosecond Crystallography of Membrane Proteins [PDF]

, 2016
Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals.
Lan, Zhu, Uwe, Weierstall, Vadim, Cherezov, Wei, Liu   +3 more
openaire   +2 more sources

Serial snapshot crystallography for materials science with SwissFEL     [PDF]

Acta Crystallographica Section A Foundations and Advances, 2015
New opportunities for studying (sub)microcrystalline materials with small unit cells, both organic and inorganic, will open up when the X-ray free electron laser (XFEL) presently being constructed in Switzerland (SwissFEL) comes online in 2017. Our synchrotron-based experiments mimicking the 4%-energy-bandpass mode of the SwissFEL beam show that it ...
Dejoie, Catherine, Smeets, Stef, Baerlocher, Christian, Tamura, Nobumichi, Pattison, Philip, Tamura, Nobumichi, Pattison, Philip, Abela, Rafael, McCusker, Lynne B.   +8 more
openaire   +10 more sources

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

Nature Communications, 2017
Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for ...
Tobias Weinert, Natacha Olieric, Robert Cheng, Steffen Brünle, Daniel James, Dmitry Ozerov, Dardan Gashi, Laura Vera, May Marsh, Kathrin Jaeger, Florian Dworkowski, Ezequiel Panepucci, Shibom Basu, Petr Skopintsev, Andrew S. Doré, Tian Geng, Robert M. Cooke, Mengning Liang, Andrea E. Prota, Valerie Panneels, Przemyslaw Nogly, Ulrich Ermler, Gebhard Schertler, Michael Hennig, Michel O. Steinmetz, Meitian Wang, Jörg Standfuss   +26 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Entering an era of dynamic structural biology…

BMC Biology, 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
doaj   +1 more source