Results 1 to 10 of about 3,503 (96)

Guide to serial synchrotron crystallography [PDF]

Current Research in Structural Biology
Serial crystallography (SX) is an emerging technique that can be used to determine the noncryogenic crystal structure of macromolecules while minimizing radiation damage.
Ki Hyun Nam
doaj   +4 more sources

Mix-and-diffuse serial synchrotron crystallography [PDF]

IUCrJ, 2017
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology.
Kenneth R. Beyerlein, Dennis Dierksmeyer, Valerio Mariani, Manuela Kuhn, Iosifina Sarrou, Angelica Ottaviano, Salah Awel, Juraj Knoska, Silje Fuglerud, Olof Jönsson, Stephan Stern, Max O. Wiedorn, Oleksandr Yefanov, Luigi Adriano, Richard Bean, Anja Burkhardt, Pontus Fischer, Michael Heymann, Daniel A. Horke, Katharina E. J. Jungnickel, Elena Kovaleva, Olga Lorbeer, Markus Metz, Jan Meyer, Andrew Morgan, Kanupriya Pande, Saravanan Panneerselvam, Carolin Seuring, Aleksandra Tolstikova, Julia Lieske, Steve Aplin, Manfred Roessle, Thomas A. White, Henry N. Chapman, Alke Meents, Dominik Oberthuer   +35 more
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3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow [PDF]

IUCrJ, 2020
Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses.
Diana C. F. Monteiro, David von Stetten, Claudia Stohrer, Marta Sans, Arwen R. Pearson, Gianluca Santoni, Peter van der Linden, Martin Trebbin   +7 more
doaj   +4 more sources

De novo protein structure determination by heavy-atom soaking in lipidic cubic phase and SIRAS phasing using serial synchrotron crystallography [PDF]

IUCrJ, 2018
During the past few years, serial crystallography methods have undergone continuous development and serial data collection has become well established at high-intensity synchrotron-radiation beamlines and XFEL radiation sources.
S. Botha, D. Baitan, K. E. J. Jungnickel, D. Oberthür, C. Schmidt, S. Stern, M. O. Wiedorn, M. Perbandt, H. N. Chapman, C. Betzel   +9 more
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Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography [PDF]

IUCrJ
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the production of ...
Sofia M. Kapetanaki, Nicolas Coquelle, David von Stetten, Martin Byrdin, Ronald Rios-Santacruz, Richard Bean, Johan Bielecki, Mohamed Boudjelida, Zsuzsana Fekete, Geoffrey W. Grime, Huijong Han, Caitlin Hatton, Sravya Kantamneni, Konstantin Kharitonov, Chan Kim, Marco Kloos, Faisal H. M. Koua, Iñaki de Diego Martinez, Diogo Melo, Lukas Rane, Adam Round, Ekaterina Round, Abhisakh Sarma, Robin Schubert, Joachim Schulz, Marcin Sikorski, Mohammad Vakili, Joana Valerio, Jovana Vitas, Raphael de Wijn, Agnieszka Wrona, Ninon Zala, Arwen Pearson, Katerina Dörner, Giorgio Schirò, Elspeth F. Garman, András Lukács, Martin Weik   +37 more
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Radiation damage and the case for unpatterned fixed targets [PDF]

IUCrJ
Advances in serial crystallography have reshaped approaches to radiation damage at room temperature. This commentary highlights recent work comparing XFEL and synchrotron experiments, emphasizing its implications for serial crystallography at modern X ...
Dominik Oberthür
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Small-rotative fixed-target serial synchrotron crystallography (SR-FT-SSX) for molecular crystals [PDF]

Communications Chemistry
The increasing availability of ultrabright Light Sources is facilitating the study of smaller crystals at faster timescales but with an increased risk of severe X-ray damage, leading to developments in multi-crystal methods such as serial crystallography
Sam G. Lewis, Ben A. Coulson, Anna J. Warren, Mark R. Warren, Lauren E. Hatcher   +4 more
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Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

Crystals, 2022
Serial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux of the pink beam of 1.2% bandwidth (BW) is one order higher than that of the monochromatic beam from a silicon crystal ...
Yongsam Kim, Ki Hyun Nam
doaj   +3 more sources

Data of serial synchrotron crystallography of xylanase GH11 from Thermoanaerobacterium saccharolyticum [PDF]

Data in Brief
The endo-1,4-β-xylanase GH11 from the hemicellulose-degrading bacterium Thermoanaerobacterium saccharolyticum (TsaGH11) has been characterized as a thermophilic enzyme.
Ki Hyun Nam
doaj   +2 more sources

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

IUCrJ, 2017
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render
Jose M. Martin-Garcia, Chelsie E. Conrad, Garrett Nelson, Natasha Stander, Nadia A. Zatsepin, James Zook, Lan Zhu, James Geiger, Eugene Chun, David Kissick, Mark C. Hilgart, Craig Ogata, Andrii Ishchenko, Nirupa Nagaratnam, Shatabdi Roy-Chowdhury, Jesse Coe, Ganesh Subramanian, Alexander Schaffer, Daniel James, Gihan Ketwala, Nagarajan Venugopalan, Shenglan Xu, Stephen Corcoran, Dale Ferguson, Uwe Weierstall, John C. H. Spence, Vadim Cherezov, Petra Fromme, Robert F. Fischetti, Wei Liu   +29 more
doaj   +3 more sources