Results 21 to 30 of about 3,647 (218)

Serial crystallography on in vivo grown microcrystals using synchrotron radiation

IUCrJ, 2014
Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized
Cornelius Gati, Gleb Bourenkov, Marco Klinge, Dirk Rehders, Francesco Stellato, Dominik Oberthür, Oleksandr Yefanov, Benjamin P. Sommer, Stefan Mogk, Michael Duszenko, Christian Betzel, Thomas R. Schneider, Henry N. Chapman, Lars Redecke   +13 more
doaj   +6 more sources

Analysis of Multi-Hit Crystals in Serial Synchrotron Crystallography Experiments Using High-Viscosity Injectors

Crystals, 2021
Serial Synchrotron Crystallography (SSX) is rapidly emerging as a promising technique for collecting data for time-resolved structural studies or for performing room temperature micro-crystallography measurements using micro-focused beamlines.
Marjan Hadian-Jazi, Peter Berntsen, Hugh Marman, Brian Abbey, Connie Darmanin   +4 more
doaj   +3 more sources

Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography [PDF]

Acta Crystallographica Section D: Structural Biology, 2021
Gabrielle Illava, Aaron D Finke, Shawn K Milano   +2 more
exaly   +2 more sources

Increasing X-ray energy improves data quality in serial crystallography [PDF]

Journal of Synchrotron Radiation
Serial synchrotron crystallography (SSX) enables structure determination from microcrystals under near-physiological, room-temperature conditions but is limited in part due to the inevitable onset of radiation damage.
Do-Heon Gu, Danny Axford, James Beilsten-Edmands, Sofia Jaho, Robin L. Owen   +4 more
doaj   +2 more sources

Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B. [PDF]

Acta Crystallogr F Struct Biol Commun, 2023
Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals.
Sharma S, Ebrahim A, Keedy DA.
europepmc   +3 more sources

Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 2020
Eugenio De La Mora, Nicolas Coquelle, Martin Rosenthal   +2 more
exaly   +2 more sources

Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources [PDF]

Structural Dynamics
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution.
Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip Johnson, Daniel James, Karol Nass, Antonia Furrer, Pikyee Ma, Steffen Bruenle, Cecilia Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly   +28 more
doaj   +2 more sources

Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. [PDF]

Sci Adv, 2021
Abstract For the two proteins myoglobin (MB) and fluoroacetate dehalogenase (FAcD), we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of crystals, the same sample delivery device, as
Mehrabi P, Bücker R, Bourenkov G, Ginn HM, von Stetten D, Müller-Werkmeister HM, Kuo A, Morizumi T, Eger BT, Ou WL, Oghbaey S, Sarracini A, Besaw JE, Pare-Labrosse O, Meier S, Schikora H, Tellkamp F, Marx A, Sherrell DA, Axford D, Owen RL, Ernst OP, Pai EF, Schulz EC, Miller RJD.   +24 more
europepmc   +7 more sources

Automated data collection and real-time data analysis suite for serial synchrotron crystallography [PDF]

Journal of Synchrotron Radiation, 2019
Shibom Basu, Ezequiel Panepucci, Chia-Ying Huang   +2 more
exaly   +2 more sources

Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. [PDF]

IUCrJ, 2022
Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus,
Zielinski KA, Prester A, Andaleeb H, Bui S, Yefanov O, Catapano L, Henkel A, Wiedorn MO, Lorbeer O, Crosas E, Meyer J, Mariani V, Domaracky M, White TA, Fleckenstein H, Sarrou I, Werner N, Betzel C, Rohde H, Aepfelbacher M, Chapman HN, Perbandt M, Steiner RA, Oberthuer D.   +23 more
europepmc   +6 more sources