Results 41 to 50 of about 3,647 (218)

Serial synchrotron crystallography for time-resolved structural biology

Current Opinion in Structural Biology, 2020
The current state-of-the-art experiments in time-resolved structural biology are undoubtedly the recent extremely impressive results that are emerging from XFEL-based experiments. However, there is a large range of macromolecular systems where the biological interest is predominantly in the slower dynamics (μs-s), that produce well diffracting ...
Pearson, A., Mehrabi, P.
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Serial crystallography captures enzyme catalysis in copper nitrite reductase at atomic resolution from one crystal

IUCrJ, 2016
Relating individual protein crystal structures to an enzyme mechanism remains a major and challenging goal for structural biology. Serial crystallography using multiple crystals has recently been reported in both synchrotron-radiation and X-ray free ...
Sam Horrell, Svetlana V. Antonyuk, Robert R. Eady, S. Samar Hasnain, Michael A. Hough, Richard W. Strange   +5 more
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Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography [PDF]

Science, 2019
Abstract Conformational dynamics are essential for proteins to function. Here we describe how we adapted time-resolved serial crystallography developed at X-ray lasers to visualize protein motions using synchrotrons. We recorded the structural changes upon proton pumping in bacteriorhodopsin over 200 ms in time.
Weinert, Tobias, Skopintsev, Petr, James, Daniel, Dworkowski, Florian, Panepucci, Ezequiel, Kekilli, Demet, Furrer, Antonia, Brünle, Steffen, Mous, Sandra, Ozerov, Dmitry, Nogly, Przemyslaw, Wang, Meitian, Standfuss, Jörg   +12 more
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Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection

IUCrJ, 2021
Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to ...
Jonathan A. Clinger, David W. Moreau, Matthew J. McLeod, Todd Holyoak, Robert E. Thorne   +4 more
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Sample delivery for serial crystallography at free-electron lasers and synchrotrons [PDF]

Acta Crystallographica Section D Structural Biology, 2019
The high peak brilliance and femtosecond pulse duration of X-ray free-electron lasers (XFELs) provide new scientific opportunities for experiments in physics, chemistry and biology. In structural biology, one of the major applications is serial femtosecond crystallography.
Grünbein, M., Nass Kovacs, G.
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Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
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Serial macromolecular crystallography at ALBA Synchrotron Light Source. Erratum

Journal of Synchrotron Radiation, 2022
A revised version of Table 2 of Martin-Garcia et al. [J. Synchrotron Rad. (2022). 29, 896–907] is provided.
Jose M. Martin-Garcia, Sabine Botha, Hao Hu, Rebecca Jernigan, Albert Castellví, Stella Lisova, Fernando Gil, Barbara Calisto, Isidro Crespo, Shatabdi Roy-Chowdhury, Alice Grieco, Gihan Ketawala, Uwe Weierstall, John Spence, Petra Fromme, Nadia Zatsepin, Dirk Roeland Boer, Xavi Carpena   +17 more
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Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Structural Dynamics, 2015
We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller, A. Marx, S. W. Epp, Y. Zhong, A. Kuo, A. R. Balo, J. Soman, F. Schotte, H. T. Lemke, R. L. Owen, E. F. Pai, A. R. Pearson, J. S. Olson, P. A. Anfinrud, O. P. Ernst, R. J. Dwayne Miller   +15 more
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Approach of Serial Crystallography

Crystals, 2020
Radiation damage and cryogenic sample environment are an experimental limitation observed in the traditional X-ray crystallography technique. However, the serial crystallography (SX) technique not only helps to determine structures at room temperature ...
Ki Hyun Nam
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Standard Sample Preparation for Serial Femtosecond Crystallography

Biomolecules
The development of serial crystallography (SX), including serial synchrotron crystallography (SSX) at synchrotron sources and serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs), has facilitated the collection of high ...
Christina Schmidt, Kristina Lorenzen, Joachim Schulz, Huijong Han   +3 more
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