Towards the spatial resolution of metalloprotein charge states by detailed modeling of XFEL crystallographic diffraction. [PDF]
, 2020 Oxidation states of individual metal atoms within a metalloprotein can be assigned by examining X-ray absorption edges, which shift to higher energy for progressively more positive valence numbers.
Holton, James M +3 more
core +2 more sources
Serial crystallography at synchrotrons and X-ray lasers [PDF]
IUCrJ, 2017 Serial crystallography was developed for the use at free-electron lasers but the approach has recently also been adapted to synchrotron sources. Here we discuss how the synergy between the two X-ray sources will facilitate a wide application of the technique in microcrystallography, room-temperature structure determination and time-resolved studies.
Jörg Standfuss, John Spence
openaire +4 more sources
Macromolecular Serial Crystallography (Volume II)
Crystals, 2022 The successful adaptation of the serial macromolecular crystallography approach at most 3rd generation synchrotron facilities allows a fruitful synergy between synchrotrons and XFELs that have accelerated the access and impact of this approach to an even
Jose M. Martin-Garcia
doaj +1 more source
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals. [PDF]
, 2020 Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful ...
Aquila, Andrew +37 more
core +2 more sources
Major Reference Works, Page 290-301., 2021 International Tables for Crystallography is the definitive resource and reference work for crystallography and structural science.
Each of the eight volumes in the series contains articles and tables of data relevant to crystallographic research and to applications of crystallographic methods in all sciences concerned with the ...
John P. Sutter C. Chantler +2 more
wiley +2 more sources
Time-resolved β-lactam cleavage by L1 metallo-β-lactamase
Nature Communications, 2022 Metallo-β-lactamases cleave β-lactam moiety of many broadly used antibiotics. Here the authors captured mechanistic details of the enzyme catalyzed reaction using time-resolved xray synchrotron serial crystallography.
M. Wilamowski +12 more
doaj +1 more source
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. [PDF]
, 2015 G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways.
Barty, Anton +71 more
core +1 more source
Crystals, 2021 The ultrabright and ultrashort pulses produced at X-ray free electron lasers (XFELs) has enabled studies of crystallized molecular machines at work under ‘native’ conditions at room temperature by the so-called time-resolved serial femtosecond ...
Jose M. Martin-Garcia
doaj +1 more source
Serial synchrotron and XFEL crystallography for studies of metalloprotein catalysis
Current Opinion in Structural Biology, 2021 An estimated half of all proteins contain a metal, with these being essential for a tremendous variety of biological functions. X-ray crystallography is the major method for obtaining structures at high resolution of these metalloproteins, but there are considerable challenges to obtain intact structures due to the effects of radiation damage.
Hough, Michael A, Owen, Robin L
openaire +3 more sources
IUCrJ, 2021 Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to ...
Jonathan A. Clinger +4 more
doaj +1 more source