IUCrJ, 2022 Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano +25 more
doaj +1 more source
Atomic resolution structure of serine protease proteinase K at ambient temperature [PDF]
, 2017 Atomic resolution structures (beyond 1.20 ?) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes.
Hatsui, T. +16 more
core +1 more source
Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals [PDF]
, 2017 We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray ...
Adriano, Luigi +24 more
core +4 more sources
Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography
Structural Dynamics, 2015 We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller +15 more
doaj +1 more source
An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography. [PDF]
, 2015 SACLAのX線自由電子レーザーを用いた新規タンパク質立体構造決定に世界で初めて成功. 京都大学プレスリリース. 2015-09-14.Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to ...
Ago, Hideo +23 more
core +1 more source
Approach of Serial Crystallography
Crystals, 2020 Radiation damage and cryogenic sample environment are an experimental limitation observed in the traditional X-ray crystallography technique. However, the serial crystallography (SX) technique not only helps to determine structures at room temperature ...
Ki Hyun Nam
doaj +1 more source
Photoreversible interconversion of a phytochrome photosensory module in the crystalline state. [PDF]
, 2020 A major barrier to defining the structural intermediates that arise during the reversible photointerconversion of phytochromes between their biologically inactive and active states has been the lack of crystals that faithfully undergo this transition ...
Aller, Pierre +26 more
core
AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy [PDF]
, 2016 X-ray crystallography is one of the main methods to determine atomic-resolution 3D images of the whole spectrum of molecules ranging from small inorganic clusters to large protein complexes consisting of hundred-thousands of atoms that constitute the ...
Berggren, Karl K +6 more
core +1 more source
A fixed-target platform for serial femtosecond crystallography in a hydrated environment. [PDF]
, 2020 For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while ...
Barthelmess, M +15 more
core
Serial crystallography using synchrotron radiation - novel strategies for microcrystallography [PDF]
Acta Crystallographica Section A Foundations and Advances, 2014 Protein crystallography continues to be one of the most frequently used techniques to obtain structural information of biomacromolecules to atomic resolution. Since protein crystals of delicate target systems are often limited in size, one of the main goals in the design of modern beamlines is the construction of highly intense X-ray beams with small ...
Cornelius Gati +9 more
openaire +1 more source