Results 71 to 80 of about 3,647 (218)

Protein crystallization and structure determination at room temperature in the CrystalChip

FEBS Open Bio
The production of high‐quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties.
Petr Pachl, Léna Coudray, Romain Vincent, Léa Nilles, Hélène Scheer, Christophe Ritzenthaler, Adéla Fejfarová, Pavlína Řezáčová, Sylvain Engilberge, Claude Sauter   +9 more
doaj   +1 more source

Novel fixed-target serial crystallography flip-holder for macromolecular crystallography beamlines at synchrotron radiation sources

Journal of Synchrotron Radiation
Synchrotron serial crystallography (SSX) is an emerging method for determining crystal structure at room temperature using synchrotron radiation facilities.
Do-Heon Gu, Dong Tak Jeong, Cheolsoo Eo, Pil-Won Seo, Jeong-Sun Kim, Suk-Youl Park   +5 more
doaj   +1 more source

A Personal Perspective on Metalloprotein Structure and the Future of Macromolecular X‐Ray Crystallography

Journal of the Chinese Chemical Society, EarlyView.
While X‐ray crystallography has been the primary method for determining the structures of metalloproteins, cryoEM has taken over this role, illustrated by [4Fe4S]‐cluster containing proteins deposited in the Protein Data Bank. What will be the role for X‐ray crystallography as cryoEM and machine learning methods dominate the initial structural analysis?
Douglas C. Rees
wiley   +1 more source

Experiencing 50 Years of Synergistic Development in Structural Biology and Enzymology

Journal of the Chinese Chemical Society, EarlyView.
Picture of Sunney Chan with Academia Sinica (AS) President James C. Liao and three Vice Presidents: Shin‐Kun Peng (left one), Tang K. Tang (right one), and Mei‐Yin Chou (right 2), taken at the annual Chinese New Year's Banquet of AS, February 7, 2025. This was likely Sunney's last appearance in formal activities of AS.
Ming‐Daw Tsai
wiley   +1 more source

The toxin–antitoxin complex Fic‐1–AntF functions as a deAMPylase that regulates the activity of DNA gyrase

mLife, EarlyView.
Abstract Toxin–antitoxin (TA) systems found in diverse bacteria play important roles in their adaptation to changing environments. The toxin of the Fic‐1–AntF TA pair from Pseudomonas bijieensis strain 2P24 inhibits bacterial DNA replication by attacking the subunit B of DNA gyrase (GyrB) via AMPylation, while the antitoxin AntF blocks its enzymatic ...
Furong Chen, Liwei Guo, Canhua Lu, Wenjun Jiang, Zhao‐Qing Luo, Junfeng Liu, Li‐Qun Zhang   +6 more
wiley   +1 more source

Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins

IUCrJ, 2019
An approach is demonstrated to obtain, in a sample- and time-efficient manner, multiple dose-resolved crystal structures from room-temperature protein microcrystals using identical fixed-target supports at both synchrotrons and X-ray free-electron lasers
Ali Ebrahim, Tadeo Moreno-Chicano, Martin V. Appleby, Amanda K. Chaplin, John H. Beale, Darren A. Sherrell, Helen M. E. Duyvesteyn, Shigeki Owada, Kensuke Tono, Hiroshi Sugimoto, Richard W. Strange, Jonathan A. R. Worrall, Danny Axford, Robin L. Owen, Michael A. Hough   +14 more
doaj   +1 more source

Mammalian Osteoderm Ultrastructure in the Armored Acomys Spiny Mouse Tail

Small, EarlyView.
The ultrastructure of armored platelets – termed osteoderms – in the tail skin of Acomys cahirinus spiny mice is characterized using 2D and 3D microscopy methods. The imbricated structure is composed of calcium phosphate biomineral and has select structural elements that are bone‐like and tooth‐like in nature.
Joseph Deering, Malcolm Maden, Marc D. McKee   +2 more
wiley   +1 more source

Large‐Scale Structural Dynamics in the Tail Fiber Modulate the Infective Transition of the T7 Bacteriophage

Small, EarlyView.
During host recognition, the fibers of T7 bacteriophages transition from a capsid bound state to an extended conformation, which requires some level of fiber flexibility. By using high‐speed atomic force microscopy, we show that the fibers have an internal molecular hinge and a torsionally compliant coiled‐coil region.
Luca Elizabet Kosik, Miklós Cervenak, Dominik Sziklai, Andrea Balogh‐Molnár, Negar Rahimi, Bence Fehér, Soma Yamamoto, Hiroki Konno, Noriyuki Kodera, Holger Flechsig, Romain Amyot, Heinz Amenitsch, Hedvig Tordai, Levente Herényi, Ana Cuervo, Miklós Kellermayer, Bálint Kiss   +16 more
wiley   +1 more source

Influence of Heat Treatment and Scan Rotation on the Microstructure of Hot Work Tool Steel Manufactured using Laser Powder Bed Fusion

steel research international, EarlyView.
The study investigates the influence of heat treatment and scan rotation on the microstructure of a modified H13 tool steel manufactured using laser powder bed fusion. Key findings include the refinement of prior austenite grains (PAGs) due to recrystallization and carbide pinning, and the significant impact of scan rotation on the size distribution of
Negar Panahi, Fengxiang Lin, Christos Oikonomou, Mikael Grehk   +3 more
wiley   +1 more source

Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron

Communications Chemistry
Serial macromolecular crystallography has become a powerful method to reveal room temperature structures of biological macromolecules and perform time-resolved studies.
Julien Orlans, Samuel L. Rose, Gavin Ferguson, Marcus Oscarsson, Alejandro Homs Puron, Antonia Beteva, Samuel Debionne, Pascal Theveneau, Nicolas Coquelle, Jerome Kieffer, Paolo Busca, Jeremy Sinoir, Victor Armijo, Marcos Lopez Marrero, Franck Felisaz, Gergely Papp, Herve Gonzalez, Hugo Caserotto, Fabien Dobias, Jonathan Gigmes, Guillaume Lebon, Shibom Basu, Daniele de Sanctis   +22 more
doaj   +1 more source