Results 91 to 100 of about 7,626 (235)

Development of Container Free Sample Exposure for Synchrotron X-ray Footprinting. [PDF]

, 2020
The method of X-ray footprinting and mass spectrometry (XFMS) on large protein assemblies and membrane protein samples requires high flux density to overcome the hydroxyl radical scavenging reactions produced by the buffer constituents and the total ...
Chen, Yan, DePonte, Daniel P, Gupta, Sayan, Petzold, Christopher J, Ralston, Corie Y   +4 more
core   +1 more source

A Synergistic Inhibitor Development Strategy Against Human UDP‐Galactose‐4‐Epimerase

Angewandte Chemie International Edition, EarlyView.
The epimerase GalE is crucial for the biosynthesis of cancer‐relevant O‐GalNAc glycans. Here, we employ orthogonal, structurally enabled small molecule fragment screens to yield both covalent and non‐covalent inhibitors against GalE within no more than 22 elaborated compounds.
William M. Browne, Jonathan Pettinger, Teresa Weckwerth, Andrew Purkiss, Sing Hei Lok, Louisa Penicaut, Roksana Ogrodowicz, Raveena Prema, Simone Kunzelmann, Chloe Roustan, Ganka Bineva‐Todd, Saskia Pieters, Francesca Zappacosta, Alfred E. Doherty, Isobel Oram, Christelle Soudy, Robert Quinlan, Joanna Redmond, Svend Kjaer, David House, Stephane Mouilleron, Jacob T. Bush, Benjamin Schumann   +22 more
wiley   +1 more source

Experimental Evidence for a Metal‐Related Function of a Cyanobactin

Angewandte Chemie International Edition, EarlyView.
Cyanobactins such as patellamides are produced by cyanobacteria profusely and have been studied extensively, but almost exclusively for their cytotoxic properties. Discussed is unprecedented experimental evidence for a metal‐related biological function – likely CO2 transport from the ascidian to the cyanobacterial symbiont.
Philipp Baur, Timothy A. Hill, Kai‐Chen Wu, Junxian Lim, Brett M. Paterson, Gunasekaran Velmurugan, Bernard M. Degnan, David P. Fairlie, Peter Comba   +8 more
wiley   +1 more source

Continuous Diffraction of Molecules and Disordered Molecular Crystals [PDF]

, 2017
The diffraction pattern of a single non-periodic compact object, such as a molecule, is continuous and is proportional to the square modulus of the Fourier transform of that object. When arrayed in a crystal, the coherent sum of the continuous diffracted
Ayyer, Kartik, Barty, Anton, Chapman, Henry N., Mariani, Valerio, Morgan, Andrew, Oberthuer, Dominik, Pande, Kanupriya, White, Thomas A., Yefanov, Oleksandr M.   +8 more
core   +3 more sources

An Open Receptor-Binding Cavity of Hemagglutinin-Esterase-Fusion Glycoprotein from Newly-Identified Influenza D Virus: Basis for Its Broad Cell Tropism. [PDF]

, 2016
Influenza viruses cause seasonal flu each year and pandemics or epidemic sporadically, posing a major threat to public health. Recently, a new influenza D virus (IDV) was isolated from pigs and cattle. Here, we reveal that the IDV utilizes 9-O-acetylated
Chen, Xi, Diaz, Sandra, Gao, George F, Khedri, Zahra, Qi, Jianxun, Shi, Yi, Song, Hao, Varki, Ajit, Yu, Hai   +8 more
core   +3 more sources

Atomically precise metal cluster enzymes for pathological tissue regeneration

BMEMat, EarlyView.
Schematic illustration of atomically precise metal cluster enzymes (MCEs) for pathological tissue regeneration. Atomically precise MCEs can modulate biological processes, such as attenuation of inflammatory responses, eradication of bacterial pathogens, regulation of angiogenesis, and promotion of cell development.
Ziqiang Xiong, Zhifeng Shi, Mengqing Li, Jien Han, Haoyan Chen, Maofei Ran, Shaojian Zhong, Limin Ma, Chengyun Ning, Youzhun Fan, Zhenghua Tang, Peng Yu   +11 more
wiley   +1 more source

Protein crystallization and structure determination at room temperature in the CrystalChip

FEBS Open Bio
The production of high‐quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties.
Petr Pachl, Léna Coudray, Romain Vincent, Léa Nilles, Hélène Scheer, Christophe Ritzenthaler, Adéla Fejfarová, Pavlína Řezáčová, Sylvain Engilberge, Claude Sauter   +9 more
doaj   +1 more source

A Personal Perspective on Metalloprotein Structure and the Future of Macromolecular X‐Ray Crystallography

Journal of the Chinese Chemical Society, EarlyView.
While X‐ray crystallography has been the primary method for determining the structures of metalloproteins, cryoEM has taken over this role, illustrated by [4Fe4S]‐cluster containing proteins deposited in the Protein Data Bank. What will be the role for X‐ray crystallography as cryoEM and machine learning methods dominate the initial structural analysis?
Douglas C. Rees
wiley   +1 more source

Hydroxyethyl cellulose matrix applied to serial crystallography [PDF]

, 2019
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the ...
Hatsui, T., Inoue, S., Iwata, S., Joti, Y., Kameshima, T., Masuda, T., Mizohata, E., Nakane, T., Nakatsu, T., Nango, E., Numata, K., Nureki, O., Song, C., Sugahara, M., Suzuki, M., Tanaka, R., Tono, K., Yabashi, M., Yumoto, F.   +18 more
core   +1 more source

Megahertz serial crystallography [PDF]

, 2018
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible.
Bean, Richard, Cruz-Mazo, Francisco, Gañán-Calvo, Alfonso M., Oberthür, Dominik, Wiedorn, Max O.   +4 more
core