Results 1 to 10 of about 1,424 (160)

Standard Sample Preparation for Serial Femtosecond Crystallography [PDF]

Biomolecules
The development of serial crystallography (SX), including serial synchrotron crystallography (SSX) at synchrotron sources and serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs), has facilitated the collection of high ...
Christina Schmidt, Kristina Lorenzen, Joachim Schulz, Huijong Han   +3 more
doaj   +5 more sources

Serial femtosecond crystallography [PDF]

Nature Reviews Methods Primers, 2022
With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing ...
Benjamin Stauch, Ilme Schlichting, Vadim Cherezov   +2 more
exaly   +6 more sources

Combination of an inject-and-transfer system for serial femtosecond crystallography [PDF]

Journal of Applied Crystallography, 2022
Abstract Serial femtosecond crystallography (SFX) enables the determination of the room-temperature crystal structure of macromolecules without causing radiation damage and provides time-resolved molecular dynamics by pump-probe experiments.
Keon Do Lee, Jaehyun Park, Wan Kyun Chung   +2 more
exaly   +6 more sources

Fixed-target serial femtosecond crystallography using in cellulo grown microcrystals [PDF]

IUCrJ, 2021
The crystallization of recombinant proteins in living cells is an exciting new approach in structural biology. Recent success has highlighted the need for fast and efficient diffraction data collection, optimally directly exposing intact crystal ...
J. Mia Lahey-Rudolph, Robert Schönherr, Miriam Barthelmess, Pontus Fischer, Carolin Seuring, Armin Wagner, Alke Meents, Lars Redecke   +7 more
doaj   +2 more sources

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin [PDF]

eLife, 2021
Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as ...
Kazumasa Oda, Takashi Nomura, Takanori Nakane, Keitaro Yamashita, Keiichi Inoue, Shota Ito, Johannes Vierock, Kunio Hirata, Andrés D Maturana, Kota Katayama, Tatsuya Ikuta, Itsuki Ishigami, Tamaki Izume, Rie Umeda, Ryuun Eguma, Satomi Oishi, Go Kasuya, Takafumi Kato, Tsukasa Kusakizako, Wataru Shihoya, Hiroto Shimada, Tomoyuki Takatsuji, Mizuki Takemoto, Reiya Taniguchi, Atsuhiro Tomita, Ryoki Nakamura, Masahiro Fukuda, Hirotake Miyauchi, Yongchan Lee, Eriko Nango, Rie Tanaka, Tomoyuki Tanaka, Michihiro Sugahara, Tetsunari Kimura, Tatsuro Shimamura, Takaaki Fujiwara, Yasuaki Yamanaka, Shigeki Owada, Yasumasa Joti, Kensuke Tono, Ryuichiro Ishitani, Shigehiko Hayashi, Hideki Kandori, Peter Hegemann, So Iwata, Minoru Kubo, Tomohiro Nishizawa, Osamu Nureki   +47 more
doaj   +2 more sources

Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE [PDF]

Structural Dynamics
X-ray Free Electron Lasers (XFELs) allow the collection of high-quality serial femtosecond crystallography data. The next generation of megahertz superconducting FELs promises to drastically reduce data collection times, enabling the capture of more ...
Samuel Perrett, Alisia Fadini, Christopher D. M. Hutchison, Sayantan Bhattacharya, Cade Morrison, Oleksii Turkot, Mads Bregenholt Jakobsen, Michael Größler, José Licón-Saláiz, Florian Griese, Samuel Flewett, Joana Valerio, Joachim Schulz, Mykola Biednov, Yifeng Jiang, Huijong Han, Hazem Yousef, Dmitry Khakhulin, Christopher Milne, Anton Barty, Jasper J. van Thor   +20 more
doaj   +2 more sources

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography [PDF]

Nature Communications, 2021
MAL and MyD88 are downstream adaptors of Toll-like receptors (TLR) and the MAL TIR domain forms filaments in vitro, which in turn nucleate the assembly of crystalline arrays of the MyD88 TIR domain.
Max T. B. Clabbers, Susannah Holmes, Timothy W. Muusse, Parimala R. Vajjhala, Sara J. Thygesen, Alpeshkumar K. Malde, Dominic J. B. Hunter, Tristan I. Croll, Leonie Flueckiger, Jeffrey D. Nanson, Md. Habibur Rahaman, Andrew Aquila, Mark S. Hunter, Mengning Liang, Chun Hong Yoon, Jingjing Zhao, Nadia A. Zatsepin, Brian Abbey, Emma Sierecki, Yann Gambin, Katryn J. Stacey, Connie Darmanin, Bostjan Kobe, Hongyi Xu, Thomas Ve   +24 more
doaj   +2 more sources

Damage before destruction? X-ray-induced changes in single-pulse serial femtosecond crystallography [PDF]

IUCrJ
Serial femtosecond crystallography (SFX) exploits extremely brief X-ray free-electron laser pulses to obtain diffraction data before destruction of the crystal.
Lewis J. Williams, Amy J. Thompson, Philipp Dijkstal, Martin Appleby, Greta Assmann, Florian S. N. Dworkowski, Nicole Hiller, Chia-Ying Huang, Tom Mason, Samuel Perrett, Eduard Prat, Didier Voulot, Bill Pedrini, John H. Beale, Michael A. Hough, Jonathan A. R. Worrall, Robin L. Owen   +16 more
doaj   +2 more sources

Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL

Photonics, 2023
Serial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage.
Jaehyun Park, Ki Hyun Nam, Park Jaehyun   +2 more
exaly   +3 more sources

Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide [PDF]

IUCrJ
Time-resolved X-ray crystallography is undergoing a renaissance due to the development of serial crystallography at synchrotron and XFEL beamlines. Crucial to such experiments are efficient and effective methods for uniformly initiating time-dependent ...
Peter Smyth, Sofia Jaho, Lewis J. Williams, Gabriel Karras, Ann Fitzpatrick, Amy J. Thompson, Sinan Battah, Danny Axford, Sam Horrell, Marina Lučić, Kotone Ishihara, Machika Kataoka, Hiroaki Matsuura, Kanji Shimba, Kensuke Tono, Takehiko Tosha, Hiroshi Sugimoto, Shigeki Owada, Michael A. Hough, Jonathan A.R. Worrall, Robin L. Owen   +20 more
doaj   +2 more sources