Results 21 to 30 of about 3,847 (182)

Double-flow focused liquid injector for efficient serial femtosecond crystallography [PDF]

Scientific Reports, 2017
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment.
Beyerlein, Kenneth R., Bushnell, David A., Knoska, Juraj, Kovaleva, Elena G. [u.v.m.], Oberthuer, Dominik, Szczepek, Michael, Wiedorn, Max O.   +6 more
core   +8 more sources

Hydroxyethyl cellulose matrix applied to serial crystallography [PDF]

Scientific Reports, 2017
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the ...
Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata   +18 more
doaj   +2 more sources

Experimental phasing of serial femtosecond crystallography data [PDF]

IUCrJ, 2017
A synopsis of and prospects for de novo phasing using diffraction data collected at X-ray free-electron lasers are given.
Ilme Schlichting
doaj   +4 more sources

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase [PDF]

IUCrJ, 2015
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage.
Raimund Fromme, Andrii Ishchenko, Markus Metz, Shatabdi Roy Chowdhury, Shibom Basu, Sébastien Boutet, Petra Fromme, Thomas A. White, Anton Barty, John C. H. Spence, Uwe Weierstall, Wei Liu, Vadim Cherezov   +12 more
doaj   +5 more sources

Room temperature structures beyond 1.5 Å by serial femtosecond crystallography

Structural Dynamics, 2015
About 2.5 × 106 snapshots on microcrystals of photoactive yellow protein (PYP) from a recent serial femtosecond crystallographic (SFX) experiment were reanalyzed to maximum resolution.
Marius Schmidt, Kanupriya Pande, Shibom Basu, Jason Tenboer   +3 more
doaj   +4 more sources

A split-beam probe-pump-probe scheme for femtosecond time resolved protein X-ray crystallography [PDF]

, 2015
In order to exploit the femtosecond pulse duration of X-ray Free-Electron Lasers (XFEL) operating in the hard X-ray regime for ultrafast time-resolved protein crystallography experiments, critical parameters that determine the crystallographic signal-to ...
Madsen, A, Van Thor, JJ
core   +8 more sources

Possibilities for serial femtosecond crystallography sample delivery at future light sources [PDF]

Structural Dynamics, 2015
Serial femtosecond crystallography (SFX) uses X-ray pulses from free-electron laser (FEL) sources that can outrun radiation damage and thereby overcome long-standing limits in the structure determination of macromolecular crystals.
L. M. G. Chavas, L. Gumprecht, H. N. Chapman   +2 more
doaj   +4 more sources

Time-resolved serial femtosecond crystallography at the European XFEL. [PDF]

Nat Methods, 2020
Published by Nature Publishing Group, London [u.a.]
Pandey S, Bean R, Sato T, Poudyal I, Bielecki J, Cruz Villarreal J, Yefanov O, Mariani V, White TA, Kupitz C, Hunter M, Abdellatif MH, Bajt S, Bondar V, Echelmeier A, Doppler D, Emons M, Frank M, Fromme R, Gevorkov Y, Giovanetti G, Jiang M, Kim D, Kim Y, Kirkwood H, Klimovskaia A, Knoska J, Koua FHM, Letrun R, Lisova S, Maia L, Mazalova V, Meza D, Michelat T, Ourmazd A, Palmer G, Ramilli M, Schubert R, Schwander P, Silenzi A, Sztuk-Dambietz J, Tolstikova A, Chapman HN, Ros A, Barty A, Fromme P, Mancuso AP, Schmidt M.   +47 more
europepmc   +7 more sources

Serial Femtosecond Crystallography of G Protein–Coupled Receptors [PDF]

Science, 2013
G Structures G protein–coupled receptors (GPCRs) are eukaryotic membrane proteins that have a central role in cellular communication and have become key drug targets. To overcome the difficulties of growing GPCRs crystals, Liu et al.
W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. A. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. H. Spence, R. C. Stevens, V. Cherezov   +32 more
openaire   +5 more sources

Polyacrylamide injection matrix for serial femtosecond crystallography. [PDF]

Sci Rep, 2019
AbstractSerial femtosecond crystallography (SFX) provides opportunities to observe the dynamics of macromolecules without causing radiation damage at room temperature. Although SFX provides a biologically more reliable crystal structure than provided by the existing synchrotron sources, there are limitations due to the consumption of many crystal ...
Park J, Park S, Kim J, Park G, Cho Y, Nam KH.   +5 more
europepmc   +4 more sources