Results 21 to 30 of about 1,362 (126)
Entering an era of dynamic structural biology…
BMC Biology, 2018 A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
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Serial crystallography is just getting started [PDF]
Structural DynamicsWhen serial crystallography at XFELs was first being developed, it was unclear how it should best be used. Early experiments included studying single crystals, or weak or small crystals, or sample delivery methods that over used crystal material or under
Aaron S Brewster +3 more
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Cooperative allostery and structural dynamics of streptavidin at cryogenic- and ambient-temperature
Communications Biology, 2022 Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature.
Esra Ayan +26 more
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Time-resolved structural studies with serial crystallography: A new light on retinal proteins
Structural Dynamics, 2015 Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy.
Valérie Panneels +17 more
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Serial femtosecond crystallography: the first five years
IUCrJ, 2015 Protein crystallography using synchrotron radiation sources has had a tremendous impact on biology, having yielded the structures of thousands of proteins and given detailed insight into their mechanisms.
Ilme Schlichting
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Nature Communications, 2016 Serial femtosecond crystallography using X-ray free-electron lasers has huge potential for time-resolved structural experiments. Here, the authors present a structure of the light-driven proton pump bacteriorhodopsin using these techniques.
Przemyslaw Nogly +39 more
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The expanding toolkit for structural biology: synchrotrons, X-ray lasers and cryoEM
IUCrJ, 2019 Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Stephen P. Muench +2 more
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Angewandte Chemie, EarlyView.Molecular recognition guest binding can comprehensively modulate photoinduced charge‐transfer dynamics in the cyclophane host, including charge separation and recombination, via coherent superexchange and incoherent hopping. Unlike covalent donor–bridge–acceptor systems, this supramolecular approach avoids tedious syntheses and offers precise tuning of
Xueze Zhao +11 more
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Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography
Structural Dynamics, 2015 We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller +15 more
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Nature Communications, 2020 rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved ...
Joyce Woodhouse +33 more
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