Results 21 to 30 of about 1,424 (160)

Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography

Applied Sciences, 2022
Serial femtosecond crystallography (SFX) using an X-ray free-electron laser (XFEL) can be applied to determine the room-temperature structure of target molecules while minimizing radiation damage and visualizing molecular dynamics.
Keondo Lee, Donghyeon Lee, Jaehyun Park, Jong-Lam Lee, Wan Kyun Chung, Yunje Cho, Ki Hyun Nam   +6 more
doaj   +1 more source

3D printed devices and infrastructure for liquid sample delivery at the European XFEL

Journal of Synchrotron Radiation, 2022
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Mohammad Vakili, Johan Bielecki, Juraj Knoška, Florian Otte, Huijong Han, Marco Kloos, Robin Schubert, Elisa Delmas, Grant Mills, Raphael de Wijn, Romain Letrun, Simon Dold, Richard Bean, Adam Round, Yoonhee Kim, Frederico A. Lima, Katerina Dörner, Joana Valerio, Michael Heymann, Adrian P. Mancuso, Joachim Schulz   +20 more
doaj   +1 more source

Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure [PDF]

Journal of Applied Crystallography, 2019
Eriko Nango, Tatsuro Shimamura, Makina Yabashi   +2 more
exaly   +2 more sources

An outlook on using serial femtosecond crystallography in drug discovery. [PDF]

Expert Opin Drug Discov, 2019
Introduction: X-ray crystallography has made important contributions to modern drug development but its application to many important drug targets has been extremely challenging. The recent emergence of X-ray free electron lasers (XFELs) and advancements in serial femtosecond crystallography (SFX) have offered new opportunities to overcome limitations ...
Mishin A, Gusach A, Luginina A, Marin E, Borshchevskiy V, Cherezov V.   +5 more
europepmc   +4 more sources

Serial Femtosecond Crystallography of G Protein–Coupled Receptors [PDF]

Science, 2013
G Structures G protein–coupled receptors (GPCRs) are eukaryotic membrane proteins that have a central role in cellular communication and have become key drug targets. To overcome the difficulties of growing GPCRs crystals, Liu et al.
W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. A. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. H. Spence, R. C. Stevens, V. Cherezov   +32 more
openaire   +3 more sources

Serial Femtosecond Crystallography of Membrane Proteins [PDF]

, 2016
Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals.
Lan, Zhu, Uwe, Weierstall, Vadim, Cherezov, Wei, Liu   +3 more
openaire   +2 more sources

A fixed-target platform for serial femtosecond crystallography in a hydrated environment. [PDF]

IUCrJ, 2020
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical challenge for some experiments, especially where this ...
Shelby ML, Gilbile D, Grant TD, Seuring C, Segelke BW, He W, Evans AC, Pakendorf T, Fischer P, Hunter MS, Batyuk A, Barthelmess M, Meents A, Coleman MA, Kuhl TL, Frank M.   +15 more
europepmc   +8 more sources

Whole-pattern fitting technique in serial femtosecond nanocrystallography

IUCrJ, 2016
Serial femtosecond X-ray crystallography (SFX) has created new opportunities in the field of structural analysis of protein nanocrystals. The intensity and timescale characteristics of the X-ray free-electron laser sources used in SFX experiments ...
Ruben A. Dilanian, Sophie R. Williams, Andrew V. Martin, Victor A. Streltsov, Harry M. Quiney   +4 more
doaj   +1 more source

Approach of Serial Crystallography

Crystals, 2020
Radiation damage and cryogenic sample environment are an experimental limitation observed in the traditional X-ray crystallography technique. However, the serial crystallography (SX) technique not only helps to determine structures at room temperature ...
Ki Hyun Nam
doaj   +1 more source