Results 1 to 10 of about 13,205,348 (309)

Strategies for sample delivery for femtosecond crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2019
Highly efficient data-collection methods are required for successful macromolecular crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is scarce, and the high peak brightness of each XFEL pulse destroys the exposed crystal volume. It is therefore necessary to combine diffraction images from a large number of crystals (
Martiel I, Müller-Werkmeister HM, Cohen AE.   +2 more
europepmc   +9 more sources

Recent Advances and Future Perspectives on Microfluidic Mix-and-Jet Sample Delivery Devices [PDF]

Micromachines, 2021
The integration of the Gas Dynamic Virtual Nozzle (GDVN) and microfluidic technologies has proven to be a promising sample delivery solution for biomolecular imaging studies and has the potential to be transformative for a range of applications in ...
Majid Hejazian, Eugeniu Balaur, Brian Abbey   +2 more
doaj   +5 more sources

Sample Delivery Media for Serial Crystallography. [PDF]

Int J Mol Sci, 2019
X-ray crystallographic methods can be used to visualize macromolecules at high resolution. This provides an understanding of molecular mechanisms and an insight into drug development and rational engineering of enzymes used in the industry. Although conventional synchrotron-based X-ray crystallography remains a powerful tool for understanding molecular
Nam KH.
europepmc   +6 more sources

Sample delivery for serial crystallography at free-electron lasers and synchrotrons. [PDF]

Acta Crystallogr D Struct Biol, 2019
The high peak brilliance and femtosecond pulse duration of X-ray free-electron lasers (XFELs) provide new scientific opportunities for experiments in physics, chemistry and biology. In structural biology, one of the major applications is serial femtosecond crystallography.
Grünbein ML, Nass Kovacs G.
europepmc   +7 more sources

Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption [PDF]

Nature Communications
Serial crystallography (SX) has revolutionized structural biology by enabling high-resolution structure determination for important classes of proteins, including the study of relevant biomolecular reaction mechanisms.
Abhik Manna, Diandra Doppler, Manasa P. Sripati, Mukul Sonker, Alexandra Ros   +4 more
doaj   +6 more sources

Rapid sample delivery for megahertz serial crystallography at X-ray FELs [PDF]

IUCrJ, 2018
Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements.
Max O. Wiedorn, Salah Awel, Andrew J. Morgan, Kartik Ayyer, Yaroslav Gevorkov, Holger Fleckenstein, Nils Roth, Luigi Adriano, Richard Bean, Kenneth R. Beyerlein, Joe Chen, Jesse Coe, Francisco Cruz-Mazo, Tomas Ekeberg, Rita Graceffa, Michael Heymann, Daniel A. Horke, Juraj Knoška, Valerio Mariani, Reza Nazari, Dominik Oberthür, Amit K. Samanta, Raymond G. Sierra, Claudiu A. Stan, Oleksandr Yefanov, Dimitrios Rompotis, Jonathan Correa, Benjamin Erk, Rolf Treusch, Joachim Schulz, Brenda G. Hogue, Alfonso M. Gañán-Calvo, Petra Fromme, Jochen Küpper, Andrei V. Rode, Saša Bajt, Richard A. Kirian, Henry N. Chapman   +37 more
doaj   +11 more sources

3D printed devices and infrastructure for liquid sample delivery at the European XFEL [PDF]

Journal of Synchrotron Radiation, 2022
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Mohammad Vakili, Johan Bielecki, Juraj Knoška, Florian Otte, Huijong Han, Marco Kloos, Robin Schubert, Elisa Delmas, Grant Mills, Raphael de Wijn, Romain Letrun, Simon Dold, Richard Bean, Adam Round, Yoonhee Kim, Frederico A. Lima, Katerina Dörner, Joana Valerio, Michael Heymann, Adrian P. Mancuso, Joachim Schulz   +20 more
doaj   +3 more sources

Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments [PDF]

Scientific Reports
Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs).
Tej Varma Yenupuri, Safi Rafie-Zinedine, Lena Worbs, Michael Heymann, Joachim Schulz, Johan Bielecki, Filipe R. N. C. Maia   +6 more
doaj   +3 more sources

Programmed sample delivery on a pressurized paper. [PDF]

Biomicrofluidics, 2014
This paper reports a method to control the fluid flow in paper-based microfluidic devices simply by pressing over the channel surface of paper, thereby decreasing the pore size and permeability of a non-woven polypropylene sheet. As a result, fluid resistance is increased in the pressed region and causes flow rate to decrease.
Shin JH, Park J, Kim SH, Park JK.
europepmc   +6 more sources

Liquid sample delivery techniques for serial femtosecond crystallography. [PDF]

Philos Trans R Soc Lond B Biol Sci, 2014
X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at ...
Weierstall U.
europepmc   +5 more sources