Liquid sample delivery techniques for serial femtosecond crystallography. [PDF]
Philos Trans R Soc Lond B Biol Sci, 2014 X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at ...
Weierstall U.
europepmc +4 more sources
PEO-sheathed liquid jets increase sample delivery stability for serial femtosecond X-ray crystallography [PDF]
Scientific ReportsViscoelastic jets can be generated by the polyethylene oxide (PEO) sheathing of an aqueous solution using double-flow focusing nozzles (DFFNs) and represent an efficient method to deliver samples that are dispersed in low and medium-viscosity liquids for
Mohammad Vakili +32 more
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Optical kicking of liquid droplets for sample delivery in ultrafast soft X-ray experiments [PDF]
Journal of Synchrotron RadiationWe present a technique based on the optical force of a femtosecond laser acting on liquid micro-droplets for their precise manipulation in a vacuum, enabling an efficient sample delivery system for soft X-ray experiments. Conventional liquid jet methods,
Zeinab Ebrahimpour +3 more
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Transdermal microconduits by microscission for drug delivery and sample acquisition [PDF]
BMC Medicine, 2004 Background Painless, rapid, controlled, minimally invasive molecular transport across human skin for drug delivery and analyte acquisition is of widespread interest.
Anderson R +4 more
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Possibilities for serial femtosecond crystallography sample delivery at future light sources [PDF]
Structural Dynamics, 2015 Serial femtosecond crystallography (SFX) uses X-ray pulses from free-electron laser (FEL) sources that can outrun radiation damage and thereby overcome long-standing limits in the structure determination of macromolecular crystals.
L. M. G. Chavas +2 more
doaj +2 more sources
Microfluidics at Fiber Tip for Nanoliter Delivery and Sampling [PDF]
Advanced Science, 2021 AbstractDelivery and sampling nanoliter volumes of liquid can benefit new invasive surgical procedures. However, the dead volume and difficulty in generating constant pressure flow limits the use of small tubes such as capillaries. This work demonstrates sub‐millimeter microfluidic chips assembled directly on the tip of a bundle of two hydrophobic ...
Antoine Barbot +3 more
openaire +4 more sources
IUCrJ, 2023 In this commentary, we explore the pioneering implementation of 3D-printed thin liquid sheet devices for advanced X-ray scattering and spectroscopy experiments at high-repetition rate XFELs.
Martin Trebbin
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Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX
IUCrJ, 2022 CmABCB1 is a Cyanidioschyzon merolae homolog of human ABCB1, a well known ATP-binding cassette (ABC) transporter responsible for multi-drug resistance in various cancers.
Dongqing Pan +11 more
doaj +1 more source
Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff
Crystals, 2020 Serial Femtosecond Crystallography (SFX) at X-ray Free electron Lasers (XFELs) is a relatively new field promising to deliver unparalleled spatial and temporal resolution on biological systems and there dynamics.
Christopher Kupitz, Raymond G. Sierra
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Radiation Damage of Polydimethylsiloxane and Polyimide by X-ray Free-Electron Laser
Applied Sciences, 2022 A crystal delivery system is essential in serial femtosecond crystallography experiments using an X-ray free-electron laser (XFEL). Investigating the XFEL-induced radiation damage to materials potentially applicable to sample delivery devices is vital ...
Keondo Lee +11 more
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