Results 31 to 40 of about 1,424 (160)

De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Nature Communications, 2022
An environmentally safe means of mosquito control is the application of Bacillus thuringiensis israelensis, which produces a cocktail of four naturally crystalline proteins exclusively toxic to mosquito.
Guillaume Tetreau, Michael R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne-Sophie Banneville, Natalie A. Schibrowsky, Nicolas Coquelle, Aaron S. Brewster, Marie Luise Grünbein, Gabriela Nass Kovacs, Mark S. Hunter, Marco Kloos, Raymond G. Sierra, Giorgio Schiro, Pei Qiao, Myriam Stricker, Dennis Bideshi, Iris D. Young, Ninon Zala, Sylvain Engilberge, Alexander Gorel, Luca Signor, Jean-Marie Teulon, Mario Hilpert, Lutz Foucar, Johan Bielecki, Richard Bean, Raphael de Wijn, Tokushi Sato, Henry Kirkwood, Romain Letrun, Alexander Batyuk, Irina Snigireva, Daphna Fenel, Robin Schubert, Ethan J. Canfield, Mario M. Alba, Frédéric Laporte, Laurence Després, Maria Bacia, Amandine Roux, Christian Chapelle, François Riobé, Olivier Maury, Wai Li Ling, Sébastien Boutet, Adrian Mancuso, Irina Gutsche, Eric Girard, Thomas R. M. Barends, Jean-Luc Pellequer, Hyun-Woo Park, Arthur D. Laganowsky, Jose Rodriguez, Manfred Burghammer, Robert L. Shoeman, R. Bruce Doak, Martin Weik, Nicholas K. Sauter, Brian Federici, Duilio Cascio, Ilme Schlichting, Jacques-Philippe Colletier   +62 more
doaj   +1 more source

Towards pump–probe single-crystal XFEL refinements for small-unit-cell systems

IUCrJ, 2023
Serial femtosecond crystallography for small-unit-cell systems has so far seen very limited application despite obvious scientific possibilities. This is because reliable data reduction has not been available for these challenging systems. In particular,
Lise Joost Støckler, Lennard Krause, Bjarke Svane, Kasper Tolborg, Bo Richter, Seiya Takahashi, Tomoki Fujita, Hidetaka Kasai, Michihiro Sugahara, Ichiro Inoue, Eiji Nishibori, Bo Brummerstedt Iversen   +11 more
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Serial Femtosecond Crystallography [PDF]

Synchrotron Radiation News, 2015
Published by Taylor & Francis, Philadelphia, Pa.
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Entering an era of dynamic structural biology…

BMC Biology, 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
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Serial crystallography is just getting started [PDF]

Structural Dynamics
When serial crystallography at XFELs was first being developed, it was unclear how it should best be used. Early experiments included studying single crystals, or weak or small crystals, or sample delivery methods that over used crystal material or under
Aaron S Brewster, Daniel W Paley, David W Mittan-Moreau, Nicholas K Sauter   +3 more
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Cooperative allostery and structural dynamics of streptavidin at cryogenic- and ambient-temperature

Communications Biology, 2022
Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature.
Esra Ayan, Busra Yuksel, Ebru Destan, Fatma Betul Ertem, Gunseli Yildirim, Meryem Eren, Oleksandr M. Yefanov, Anton Barty, Alexandra Tolstikova, Gihan K. Ketawala, Sabine Botha, E. Han Dao, Brandon Hayes, Mengning Liang, Matthew H. Seaberg, Mark S. Hunter, Alexander Batyuk, Valerio Mariani, Zhen Su, Frederic Poitevin, Chun Hong Yoon, Christopher Kupitz, Aina Cohen, Tzanko Doukov, Raymond G. Sierra, Çağdaş Dağ, Hasan DeMirci   +26 more
doaj   +1 more source

Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural Dynamics, 2015
Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy.
Valérie Panneels, Wenting Wu, Ching-Ju Tsai, Przemek Nogly, Jan Rheinberger, Kathrin Jaeger, Gregor Cicchetti, Cornelius Gati, Leonhard M. Kick, Leonardo Sala, Guido Capitani, Chris Milne, Celestino Padeste, Bill Pedrini, Xiao-Dan Li, Jörg Standfuss, Rafael Abela, Gebhard Schertler   +17 more
doaj   +1 more source

Lipidic phase membrane protein serial femtosecond crystallography [PDF]

Nature Methods, 2012
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center ...
Johansson L. C., Arnlund D., White T. A., Katona G., Deponte D. P., Weierstall U., Doak R. B., Shoeman R. L., Lomb L., Malmerberg E., Davidsson J., Nass K., Liang M., Andreasson J., Aquila A., Bajt S., Barthelmess M., Barty A., Bogan M. J., Bostedt C., Bozek J. D., Caleman C., Coffee R., Coppola N., Ekeberg T., Epp S. W., Erk B., Fleckenstein H., Foucar L., Graafsma H., Gumprecht L., Hajdu J., Hampton C. Y., Hartmann R., Hartmann A., Hauser G., Hirsemann H., Holl P., Hunter M. S., Kassemeyer S., Kimmel N., Kirian R. A., Maia F. R. N. C., Marchesini S., Martin A. V., Reich C., Rolles D., Rudek B., Rudenko A., Schlichting I., Schulz J., Seibert M. M., Sierra R. G., Soltau H., Starodub D., Stellato F., Stern S., Strulder L., Timneanu N., Ullrich J., Wahlgren W. Y., Wang X., Weidenspointner G., Wunderer C., Fromme P., Chapman H. N., Spence J. C. H., Neutze R.   +67 more
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Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography [PDF]

Cell, 2015
Angiotensin II type 1 receptor (AT(1)R) is a G protein-coupled receptor that serves as a primary regulator for blood pressure maintenance. Although several anti-hypertensive drugs have been developed as AT(1)R blockers (ARBs), the structural basis for AT(1)R ligand-binding and regulation has remained elusive, mostly due to the difficulties of growing ...
Zhang, Haitao, Unal, Hamiyet, Gati, Cornelius, Han, Gye Won, Liu, Wei, Zatsepin, Nadia A., James, Daniel, Wang, Dingjie, Nelson, Garrett, Weierstall, Uwe, Sawaya, Michael R., Xu, Qingping, Messerschmidt, Marc, Williams, Garth J., Boutet, Sébastien, Yefanov, Oleksandr M., White, Thomas A., Wang, Chong, Ishchenko, Andrii, Tirupula, Kalyan C., Desnoyer, Russell, Coe, Jesse, Conrad, Chelsie E., Fromme, Petra, Stevens, Raymond C., Katritch, Vsevolod, Karnik, Sadashiva S., Cherezov, Vadim   +27 more
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Data processing pipeline for serial femtosecond crystallography at SACLA [PDF]

Journal of Applied Crystallography, 2016
A data processing pipeline for serial femtosecond crystallography at SACLA was developed, based onCheetah[Bartyet al.(2014).J. Appl. Cryst.47, 1118–1131] andCrystFEL[Whiteet al.(2016).J. Appl. Cryst.49, 680–689]. The original programs were adapted for data acquisition through the SACLA API, thread and inter-node parallelization, and efficient image ...
Nakane, Takanori, Joti, Yasumasa, Tono, Kensuke, Yabashi, Makina, Nango, Eriko, Iwata, So, Ishitani, Ryuichiro, Nureki, Osamu   +7 more
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