Results 31 to 40 of about 3,847 (182)

Nanoflow electrospinning serial femtosecond crystallography [PDF]

Acta Crystallographica Section D Biological Crystallography, 2012
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or ...
Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J.   +37 more
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Serial X-ray Crystallography

Crystals, 2022
Serial crystallography (SX) is an emerging technique to determine macromolecules at room temperature. SX with a pump–probe experiment provides the time-resolved dynamics of target molecules.
Ki Hyun Nam
doaj   +1 more source

Asymmetry in serial femtosecond crystallography data [PDF]

Acta Crystallographica Section A Foundations and Advances, 2017
Serial crystallography is an increasingly important approach to protein crystallography that exploits both X-ray free-electron laser (XFEL) and synchrotron radiation. Serial crystallography recovers complete X-ray diffraction data by processing and merging diffraction images from thousands of randomly oriented non-uniform microcrystals, of which all ...
Amit Sharma, Linda Johansson, Elin Dunevall, Weixiao Y. Wahlgren, Richard Neutze, Gergely Katona   +5 more
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Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography

Applied Sciences, 2022
Serial femtosecond crystallography (SFX) using an X-ray free-electron laser (XFEL) can be applied to determine the room-temperature structure of target molecules while minimizing radiation damage and visualizing molecular dynamics.
Keondo Lee, Donghyeon Lee, Jaehyun Park, Jong-Lam Lee, Wan Kyun Chung, Yunje Cho, Ki Hyun Nam   +6 more
doaj   +1 more source

3D printed devices and infrastructure for liquid sample delivery at the European XFEL

Journal of Synchrotron Radiation, 2022
The Sample Environment and Characterization (SEC) group of the European X-ray Free-Electron Laser (EuXFEL) develops sample delivery systems for the various scientific instruments, including systems for the injection of liquid samples that enable serial ...
Mohammad Vakili, Johan Bielecki, Juraj Knoška, Florian Otte, Huijong Han, Marco Kloos, Robin Schubert, Elisa Delmas, Grant Mills, Raphael de Wijn, Romain Letrun, Simon Dold, Richard Bean, Adam Round, Yoonhee Kim, Frederico A. Lima, Katerina Dörner, Joana Valerio, Michael Heymann, Adrian P. Mancuso, Joachim Schulz   +20 more
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Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

Whole-pattern fitting technique in serial femtosecond nanocrystallography

IUCrJ, 2016
Serial femtosecond X-ray crystallography (SFX) has created new opportunities in the field of structural analysis of protein nanocrystals. The intensity and timescale characteristics of the X-ray free-electron laser sources used in SFX experiments ...
Ruben A. Dilanian, Sophie R. Williams, Andrew V. Martin, Victor A. Streltsov, Harry M. Quiney   +4 more
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De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Nature Communications, 2022
An environmentally safe means of mosquito control is the application of Bacillus thuringiensis israelensis, which produces a cocktail of four naturally crystalline proteins exclusively toxic to mosquito.
Guillaume Tetreau, Michael R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne-Sophie Banneville, Natalie A. Schibrowsky, Nicolas Coquelle, Aaron S. Brewster, Marie Luise Grünbein, Gabriela Nass Kovacs, Mark S. Hunter, Marco Kloos, Raymond G. Sierra, Giorgio Schiro, Pei Qiao, Myriam Stricker, Dennis Bideshi, Iris D. Young, Ninon Zala, Sylvain Engilberge, Alexander Gorel, Luca Signor, Jean-Marie Teulon, Mario Hilpert, Lutz Foucar, Johan Bielecki, Richard Bean, Raphael de Wijn, Tokushi Sato, Henry Kirkwood, Romain Letrun, Alexander Batyuk, Irina Snigireva, Daphna Fenel, Robin Schubert, Ethan J. Canfield, Mario M. Alba, Frédéric Laporte, Laurence Després, Maria Bacia, Amandine Roux, Christian Chapelle, François Riobé, Olivier Maury, Wai Li Ling, Sébastien Boutet, Adrian Mancuso, Irina Gutsche, Eric Girard, Thomas R. M. Barends, Jean-Luc Pellequer, Hyun-Woo Park, Arthur D. Laganowsky, Jose Rodriguez, Manfred Burghammer, Robert L. Shoeman, R. Bruce Doak, Martin Weik, Nicholas K. Sauter, Brian Federici, Duilio Cascio, Ilme Schlichting, Jacques-Philippe Colletier   +62 more
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Serial Femtosecond Crystallography of Membrane Proteins [PDF]

, 2016
Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals.
Lan, Zhu, Uwe, Weierstall, Vadim, Cherezov, Wei, Liu   +3 more
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Approach of Serial Crystallography

Crystals, 2020
Radiation damage and cryogenic sample environment are an experimental limitation observed in the traditional X-ray crystallography technique. However, the serial crystallography (SX) technique not only helps to determine structures at room temperature ...
Ki Hyun Nam
doaj   +1 more source