Results 51 to 60 of about 3,847 (182)

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nature Communications, 2016
Serial femtosecond crystallography using X-ray free-electron lasers has huge potential for time-resolved structural experiments. Here, the authors present a structure of the light-driven proton pump bacteriorhodopsin using these techniques.
Przemyslaw Nogly, Valerie Panneels, Garrett Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie Conrad, Jesse Coe, Richard Bean, Yun Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, Kenneth R. Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonte, Chufeng Li, Leonardo Sala, Garth J. Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry N. Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard Schertler, Jörg Standfuss   +39 more
doaj   +1 more source

A Bright Future for Serial Femtosecond Crystallography with XFELs. [PDF]

Trends Biochem Sci, 2017
X-ray free electron lasers (XFELs) have the potential to revolutionize macromolecular structural biology due to the unique combination of spatial coherence, extreme peak brilliance, and short duration of X-ray pulses. A recently emerged serial femtosecond (fs) crystallography (SFX) approach using XFEL radiation overcomes some of the biggest hurdles of ...
Johansson LC, Stauch B, Ishchenko A, Cherezov V.   +3 more
europepmc   +4 more sources

Atomic resolution structure of serine protease proteinase K at ambient temperature [PDF]

, 2017
Atomic resolution structures (beyond 1.20 ?) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes.
Hatsui, T., Inoue, S., Iwata, S., Joti, Y., Kameshima, T., Masuda, T., Mikami, B., Nakane, T., Nango, E., Numata, K., Nureki, O., Song, C., Sugahara, M., Suzuki, M., Tanaka, R., Tono, K., Yabashi, M.   +16 more
core   +1 more source

Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin [PDF]

Scientific Data, 2017
AbstractWe provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination.
Yoon, Chun Hong, DeMirci, Hasan, Sierra, Raymond G., Dao, E. Han, Ahmadi, Radman, Aksit, Fulya, Aquila, Andrew L., Batyuk, Alexander, Ciftci, Halilibrahim, Guillet, Serge, Hayes, Matt J., Hayes, Brandon, Lane, Thomas J., Liang, Meng, Lundström, Ulf, Koglin, Jason E., Mgbam, Paul, Rao, Yashas, Rendahl, Theodore, Rodriguez, Evan, Zhang, Lindsey, Wakatsuki, Soichi, Boutet, Sébastien, Holton, James M., Hunter, Mark S.   +24 more
openaire   +2 more sources

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography. [PDF]

, 2015
SACLAのX線自由電子レーザーを用いた新規タンパク質立体構造決定に世界で初めて成功. 京都大学プレスリリース. 2015-09-14.Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) holds great potential for structure determination of challenging proteins that are not amenable to ...
Ago, Hideo, Gomi, Keiko, Hatsui, Takaki, Iwata, So, Joti, Yasumasa, Kajiyama, Naoki, Kameshima, Takashi, Kato, Hiroaki, Kodan, Atsushi, Mizohata, Eiichi, Murai, Tomohiro, Nakatsu, Toru, Nango, Eriko, Okuda, Tomohiko, Pan, Dongqing, Park, Jaehyun, Song, Changyong, Sugahara, Michihiro, Suzuki, Mamoru, Tono, Kensuke, Yabashi, Makina, Yamaguchi, Tomohiro, Yamamoto, Masaki, Yamashita, Keitaro   +23 more
core   +1 more source

Mapping Atomic Motions with Electrons: Toward the Quantum Limit to Imaging Chemistry [PDF]

, 2020
Recent advances in ultrafast electron and X-ray diffraction have pushed imaging of structural dynamics into the femtosecond time domain, that is, the fundamental time scale of atomic motion.
Atkins P. W., Born M., Goodhew P. J., Leonhardt U., McQuarrie D. A., Miller R. J. D., Natterer F., Pauli W., Shih Y., Yang J.   +9 more
core   +2 more sources

Serial femtosecond crystallography opens new avenues for Structural Biology [PDF]

Protein & Peptide Letters, 2016
Free electron lasers (FELs) provide X-ray pulses in the femtosecond time domain with up to 10(12) higher photon flux than synchrotrons and open new avenues for the determination of difficult to crystallize proteins, like large complexes and human membrane proteins.
Jesse, Coe, Petra, Fromme
openaire   +2 more sources

The expanding toolkit for structural biology: synchrotrons, X-ray lasers and cryoEM

IUCrJ, 2019
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Stephen P. Muench, Svetlana V. Antonyuk, S. Samar Hasnain   +2 more
doaj   +1 more source

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography [PDF]

Acta Crystallographica Section D Biological Crystallography, 2015
Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å ...
Nakane, T, Song, CY, Suzuki, M, Nango, E, Kobayashi, J, Masuda, T, Inoue, S, Mizohata, E, Nakatsu, T, Tanaka, T, Tanaka, R, Shimamura, T, Tono, K, Joti, Y, Kameshima, T, Hatsui, T, Yabashi, M, Nureki, O, Iwata, S, Sugahara, M   +19 more
openaire   +3 more sources

Photoreversible interconversion of a phytochrome photosensory module in the crystalline state. [PDF]

, 2020
A major barrier to defining the structural intermediates that arise during the reversible photointerconversion of phytochromes between their biologically inactive and active states has been the lack of crystals that faithfully undergo this transition ...
Aller, Pierre, Alonso-Mori, Roberto, Batyuk, Alexander, Bhowmick, Asmit, Brewster, Aaron S, Burgie, E Sethe, Butryn, Agata, Clinger, Jonathan A, Cohen, Aina E, Fuller, Franklin D, Gul, Sheraz, Heinemann, Joshua V, Hunter, Mark S, Kern, Jan, Kim, In-Sik, Koglin, Jason E, Miller, Mitchell D, O'Riordan, Lee J, Orville, Allen M, Pham, Cindy C, Phillips, George N, Sauter, Nicholas K, Sutherlin, Kyle D, Vierstra, Richard D, Yachandra, Vittal K, Yano, Junko, Young, Iris D   +26 more
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