Results 71 to 80 of about 3,847 (182)

High-speed fixed-target serial virus crystallography [PDF]

, 2017
We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with
Alonso-Mori, R, Brewster, AS, Damiani, DS, David, C, Duman, R, Fischer, P, Fry, EE, Ginn, HM, Harlos, K, Kelly, J, Kotecha, A, Meents, A, Meyer, J, Michels-Clark, T, Nelson, S, Pakendorf, T, Reime, B, Ren, J, Roedig, P, Rowlands, DJ, Sauter, NK, Sikorsky, M, Stuart, DI, Sutton, G, Vartiainen, I, Wagner, A, Walter, TS, Warmer, M, Young, ID   +28 more
core   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Protein Dynamics and Time Resolved Protein Crystallography at Synchrotron Radiation Sources: Past, Present and Future

Crystals, 2021
The ultrabright and ultrashort pulses produced at X-ray free electron lasers (XFELs) has enabled studies of crystallized molecular machines at work under ‘native’ conditions at room temperature by the so-called time-resolved serial femtosecond ...
Jose M. Martin-Garcia
doaj   +1 more source

Azaporphyrinoid‐Based Photo‐ and Electroactive Architectures for Advanced Functional Materials

Advanced Materials, EarlyView.
A long‐standing collaboration between the Torres and Guldi groups has yielded diverse azaporphyrinoid‐based donor‐acceptor nanohybrids with promising applications in solar energy conversion. This conspectus highlights key molecular platforms and structure‐function relationships that govern light and charge management, supporting the rational design of ...
Jorge Labella, Kobra Azizi, Dirk M. Guldi, Tomás Torres   +3 more
wiley   +1 more source

Characterizing gas flow from aerosol particle injectors

, 2017
A novel methodology for measuring gas flow from small orifices or nozzles into vacuum is presented. It utilizes a high-intensity femtosecond laser pulse to create a plasma within the gas plume produced by the nozzle, which is imaged by a microscope ...
Horke, Daniel, Küpper, Jochen, Roth, Nils, Worbs, Lena   +3 more
core   +2 more sources

Ternary structure reveals mechanism of a membrane diacylglycerol kinase [PDF]

, 2015
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 residues per subunit, means that available ...
Aragao, David, Bandaru, Sateesh, Barty, Anton, Basu, Shibom, Boutet, Sébastien, Caffrey, Martin, Chapman, Henry N., Cherezov, Vadim, Diederichs, Kay, English, Niall J., Fromme, Petra, Fromme, Raimund, Gati, Cornelius, Grotjohann, Ingo, Howe, Nicole, Keogh, Aaron, Kupitz, Christopher, Li, Dianfan, Liu, Wei, Lyons, Joseph A., Marvin Seibert, M., Messerschmidt, Marc, Rendek, Kimberley, Sansom, Mark S. P., Stansfeld, Phillip J., Vogeley, Lutz, Weierstall, Uwe, Williams, Garth J., Yefanov, Oleksandr, Zatsepin, Nadia A.   +29 more
core   +2 more sources

2D Nanomaterials Toward Function‐Ready Superlubricity in Advanced Microsystems

Advanced Materials, EarlyView.
A unified framework links structural and transformation superlubricity with microsystem functions and deployment requirements. Mechanisms, device architectures, integration strategies, AI‐guided discovery, and benchmarking protocols are connected to define function‐ready superlubricity in advanced microsystems.
Yushan Geng, Jun Yang, Yong Yang
wiley   +1 more source

Weakly supervised learning for pattern classification in serial femtosecond crystallography

Optics Express, 2023
Serial femtosecond crystallography at X-ray free electron laser facilities opens a new era for the determination of crystal structure. However, the data processing of those experiments is facing unprecedented challenge, because the total number of diffraction patterns needed to determinate a high-resolution structure is huge.
Jianan Xie, Ji Liu, Chi Zhang, Xihui Chen, Ping Huai, Jie Zheng, Xiaofeng Zhang   +6 more
openaire   +3 more sources

Guide to serial synchrotron crystallography

Current Research in Structural Biology
Serial crystallography (SX) is an emerging technique that can be used to determine the noncryogenic crystal structure of macromolecules while minimizing radiation damage.
Ki Hyun Nam
doaj   +1 more source

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

Nature Communications, 2020
Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond ...
Guillaume Tetreau, Anne-Sophie Banneville, Elena A. Andreeva, Aaron S. Brewster, Mark S. Hunter, Raymond G. Sierra, Jean-Marie Teulon, Iris D. Young, Niamh Burke, Tilman A. Grünewald, Joël Beaudouin, Irina Snigireva, Maria Teresa Fernandez-Luna, Alister Burt, Hyun-Woo Park, Luca Signor, Jayesh A. Bafna, Rabia Sadir, Daphna Fenel, Elisabetta Boeri-Erba, Maria Bacia, Ninon Zala, Frédéric Laporte, Laurence Després, Martin Weik, Sébastien Boutet, Martin Rosenthal, Nicolas Coquelle, Manfred Burghammer, Duilio Cascio, Michael R. Sawaya, Mathias Winterhalter, Enrico Gratton, Irina Gutsche, Brian Federici, Jean-Luc Pellequer, Nicholas K. Sauter, Jacques-Philippe Colletier   +37 more
doaj   +1 more source