Results 61 to 70 of about 1,424 (160)

Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography [PDF]

Scientific Reports, 2018
Abstract Time-resolved serial femtosecond crystallography with X-ray free electron laser (XFEL) holds the potential to view fast reactions occurring at near-physiological temperature. However, production and characterization of homogeneous micron-sized protein crystals at high density remain a bottleneck, due to the lack of the ...
Lee, Dan Bi, Kim, Jong-Min, Seok, Jong Hyeon, Lee, Ji-Hye, Jo, Jae Deok, Mun, Ji Young, Conrad, Chelsie, Coe, Jesse, Nelson, Gerrett, Hogue, Brenda, White, Thomas A., Zatsepin, Nadia, Weierstall, Uwe, Barty, Anton, Chapman, Henry, Fromme, Petra, Spence, John, Chung, Mi Sook, Oh, Chang-Hyun, Kim, Kyung Hyun   +19 more
openaire   +4 more sources

Weakly supervised learning for pattern classification in serial femtosecond crystallography

Optics Express, 2023
Serial femtosecond crystallography at X-ray free electron laser facilities opens a new era for the determination of crystal structure. However, the data processing of those experiments is facing unprecedented challenge, because the total number of diffraction patterns needed to determinate a high-resolution structure is huge.
Jianan Xie, Ji Liu, Chi Zhang, Xihui Chen, Ping Huai, Jie Zheng, Xiaofeng Zhang   +6 more
openaire   +3 more sources

Graphs of protein-water hydrogen bond networks to dissect structural movies of ion-transfer microbial rhodopsins

Frontiers in Chemistry, 2023
Microbial rhodopsins are membrane proteins that use the energy absorbed by the covalently bound retinal chromophore to initiate reaction cycles resulting in ion transport or signal transduction.
Éva Bertalan, Ana-Nicoleta Bondar, Ana-Nicoleta Bondar   +2 more
doaj   +1 more source

Phase Engineering of Atomically Precise Nanoclusters (APNCs) of Gold and Beyond

Advanced Materials, EarlyView.
Engineering the structural phase of materials is of paramount importance for both fundamental research and practical applications. In this Review, we summarize the recent progress in controlling the phases of atomically precise nanoclusters (APNCs) of gold, silver and copper, as well as bimetallic systems. The phase‐enabled material properties of APNCs
Yitong Wang, Avirup Sardar, Weijie Ji, Zhongyu Liu, Rongchao Jin   +4 more
wiley   +1 more source

Conjugated Metal‐Organic Frameworks on Laser‐Induced‐Graphene Scaffold Enable Picomolar Sensitivity in Electrical Biosensors

Advanced Materials Interfaces, EarlyView.
A controlled layer‐by‐layer growth process integrates conjugated metal‐organic framework thin films onto a hierarchical 3D laser‐induced graphene scaffold. When incorporated into an extended‐gate field‐effect transistor configuration, this synergistic hybrid platform achieves robust aptamer immobilization, driving highly selective, picomolar‐level ...
Khanh T. M. Le, Tuan‐Khoa Nguyen, Nam‐Trung Nguyen   +2 more
wiley   +1 more source

Regioselective π‐Extension of Helical PAHs: Tuning Energy Gaps and Excited‐State Dynamics Directed by Clar's Sextet Migration

Advanced Optical Materials, EarlyView.
Central and terminal ring fusions of per‐peri‐perbenzo[7]helicene (PB[7]H) yield novel helical polycyclic aromatic hydrocarbons (HPAHs). The migration of Clar's aromatic sextets governs the inner‐rim stiffness. This topology‐directed strategy bridges the discrete optical gaps of homologous series. Transition moment density (TMD) analysis visualizes the
Zhibo Liu, Zheng Zhang, Hikaru Sotome, Takashi Hirose   +3 more
wiley   +1 more source

Serial femtosecond crystallography at the SACLA: breakthrough to dynamic structural biology [PDF]

Biophysical Reviews, 2017
X-ray crystallography visualizes the world at the atomic level. It has been used as the most powerful technique for observing the three-dimensional structures of biological macromolecules and has pioneered structural biology. To determine a crystal structure with high resolution, it was traditionally required to prepare large crystals (> 200 μm). Later,
Eiichi, Mizohata, Takanori, Nakane, Yohta, Fukuda, Eriko, Nango, So, Iwata   +4 more
openaire   +2 more sources

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

Oil-free hyaluronic acid matrix for serial femtosecond crystallography [PDF]

Scientific Reports, 2016
AbstractThe grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins.
Sugahara, M, Song, C, Suzuki, M, Masuda, T, Inoue, S, Nakane, T, Yumoto, F, Nango, E, Tanaka, R, Tono, K, Joti, Y, Kameshima, T, Hatsui, T, Yabashi, M, Nureki, O, Numata, K, Iwata, S.   +16 more
openaire   +3 more sources

Demonstration of femtosecond X-ray pump X-ray probe diffraction on protein crystals

Structural Dynamics, 2018
The development of X-ray free-electron lasers (XFELs) has opened the possibility to investigate the ultrafast dynamics of biomacromolecules using X-ray diffraction.
Nadia L. Opara, Istvan Mohacsi, Mikako Makita, Daniel Castano-Diez, Ana Diaz, Pavle Juranić, May Marsh, Alke Meents, Christopher J. Milne, Aldo Mozzanica, Celestino Padeste, Valérie Panneels, Marcin Sikorski, Sanghoon Song, Henning Stahlberg, Ismo Vartiainen, Laura Vera, Meitian Wang, Philip R. Willmott, Christian David   +19 more
doaj   +1 more source