Results 61 to 70 of about 3,847 (182)

AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy [PDF]

, 2016
X-ray crystallography is one of the main methods to determine atomic-resolution 3D images of the whole spectrum of molecules ranging from small inorganic clusters to large protein complexes consisting of hundred-thousands of atoms that constitute the ...
Berggren, Karl K, Hobbs, Richard, Huang, W. R., Kaertner, Franz X, Nanni, Emilio Alessandro, Putnam, William P, Ravi, Koustuban   +6 more
core   +1 more source

Serial femtosecond crystallography datasets from G protein-coupled receptors [PDF]

Scientific Data, 2016
AbstractWe describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with
White, Thomas A., Barty, Anton, Liu, Wei, Ishchenko, Andrii, Zhang, Haitao, Gati, Cornelius, Zatsepin, Nadia A., Basu, Shibom, Oberthür, Dominik, Metz, Markus, Beyerlein, Kenneth R., Yoon, Chun Hong, Yefanov, Oleksandr M., James, Daniel, Wang, Dingjie, Messerschmidt, Marc, Koglin, Jason E., Boutet, Sébastien, Weierstall, Uwe, Cherezov, Vadim   +19 more
openaire   +4 more sources

Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Structural Dynamics, 2015
We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller, A. Marx, S. W. Epp, Y. Zhong, A. Kuo, A. R. Balo, J. Soman, F. Schotte, H. T. Lemke, R. L. Owen, E. F. Pai, A. R. Pearson, J. S. Olson, P. A. Anfinrud, O. P. Ernst, R. J. Dwayne Miller   +15 more
doaj   +1 more source

Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

Nature Communications, 2020
rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved ...
Joyce Woodhouse, Gabriela Nass Kovacs, Nicolas Coquelle, Lucas M. Uriarte, Virgile Adam, Thomas R. M. Barends, Martin Byrdin, Eugenio de la Mora, R. Bruce Doak, Mikolaj Feliks, Martin Field, Franck Fieschi, Virginia Guillon, Stefan Jakobs, Yasumasa Joti, Pauline Macheboeuf, Koji Motomura, Karol Nass, Shigeki Owada, Christopher M. Roome, Cyril Ruckebusch, Giorgio Schirò, Robert L. Shoeman, Michel Thepaut, Tadashi Togashi, Kensuke Tono, Makina Yabashi, Marco Cammarata, Lutz Foucar, Dominique Bourgeois, Michel Sliwa, Jacques-Philippe Colletier, Ilme Schlichting, Martin Weik   +33 more
doaj   +1 more source

Oil-free hyaluronic acid matrix for serial femtosecond crystallography [PDF]

Scientific Reports, 2016
AbstractThe grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins.
Sugahara, M, Song, C, Suzuki, M, Masuda, T, Inoue, S, Nakane, T, Yumoto, F, Nango, E, Tanaka, R, Tono, K, Joti, Y, Kameshima, T, Hatsui, T, Yabashi, M, Nureki, O, Numata, K, Iwata, S.   +16 more
openaire   +3 more sources

Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals [PDF]

, 2017
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray ...
Adriano, Luigi, Aquila, Andrew, Awel, Salah, Bajt, Saša, Barty, Anton, Beyerlein, Kenneth R., Chapman, Henry N., Horke, Daniel A., Hunter, Mark S., James, Daniel, Kirian, Richard A., Knoska, Juraj, Küpper, Jochen, Mariani, Valerio, Morgan, Andrew, Oberthür, Dominik, Robinson, Joseph S., Rode, Andrei V., Roth, Nils, Roy-Chowdhury, Shatabdi, Tolstikova, Alexandra, Weierstall, Uwe, Wiedorn, Max O., Xavier, P. Lourdu, Yefanov, Oleksandr   +24 more
core   +4 more sources

Data processing pipeline for serial femtosecond crystallography at SACLA [PDF]

Journal of Applied Crystallography, 2016
A data processing pipeline for serial femtosecond crystallography at SACLA was developed, based onCheetah[Bartyet al.(2014).J. Appl. Cryst.47, 1118–1131] andCrystFEL[Whiteet al.(2016).J. Appl. Cryst.49, 680–689]. The original programs were adapted for data acquisition through the SACLA API, thread and inter-node parallelization, and efficient image ...
Nakane, Takanori, Joti, Yasumasa, Tono, Kensuke, Yabashi, Makina, Nango, Eriko, Iwata, So, Ishitani, Ryuichiro, Nureki, Osamu   +7 more
openaire   +2 more sources

Sample Delivery Systems for Serial Femtosecond Crystallography at the PAL-XFEL

Photonics, 2023
Serial femtosecond crystallography (SFX) using an X-ray free electron laser (XFEL) enables the determination of room-temperature structures without causing radiation damage.
Jaehyun Park, Ki Hyun Nam
doaj   +1 more source

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography [PDF]

Structure, 2016
X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter
Roessler, Christian G, Agarwal, Rakhi, Allaire, Marc, Alonso-Mori, Roberto, Andi, Babak, Bachega, José FR, Bommer, Martin, Brewster, Aaron S, Browne, Michael C, Chatterjee, Ruchira, Cho, Eunsun, Cohen, Aina E, Cowan, Matthew, Datwani, Sammy, Davidson, Victor L, Defever, Jim, Eaton, Brent, Ellson, Richard, Feng, Yiping, Ghislain, Lucien P, Glownia, James M, Han, Guangye, Hattne, Johan, Hellmich, Julia, Héroux, Annie, Ibrahim, Mohamed, Kern, Jan, Kuczewski, Anthony, Lemke, Henrik T, Liu, Pinghua, Majlof, Lars, McClintock, William M, Myers, Stuart, Nelsen, Silke, Olechno, Joe, Orville, Allen M, Sauter, Nicholas K, Soares, Alexei S, Soltis, S Michael, Song, Heng, Stearns, Richard G, Tran, Rosalie, Tsai, Yingssu, Uervirojnangkoorn, Monarin, Wilmot, Carrie M, Yachandra, Vittal, Yano, Junko, Yukl, Erik T, Zhu, Diling, Zouni, Athina   +49 more
openaire   +4 more sources

Quantum Imaging with Incoherently Scattered Light from a Free-Electron Laser [PDF]

, 2017
The advent of accelerator-driven free-electron lasers (FEL) has opened new avenues for high-resolution structure determination via diffraction methods that go far beyond conventional x-ray crystallography methods.
A Aquila, A Barty, A Classen, A Singer, Adrian Benz, Anton Classen, Birgit Fischer, C Kupitz, C Thiel, Daniel Bhatti, Felix Waldmann, G Baym, Giuseppe Mercurio, Günter Brenner, HN Chapman, HN Chapman, HN Chapman, I Barke, Ivan A. Vartanyants, Ivan Zaluzhnyy, JM Slovik, Joachim von Zanthier, Jochen Wagner, JW Goodman, JW Goodman, K Ayyer, Kai Schlage, Lars Bocklage, Lukas Wenthaus, M Seibert, ND Loh, Oleg Gorobtsov, OY Gorobtsov, OY Gorobtsov, Petr Skopintsev, R Hanbury Brown, R Hanbury Brown, R Neutze, Raimund Schneider, Ralf Röhlsberger, RJ Glauber, RJ Glauber, S Oppel, Sergey Lazarev, Svenja Willing, Thomas Mehringer, W Ackermann, Wilfried Wurth, Y Takahashi, Yuri Obukhov   +49 more
core   +2 more sources