Results 61 to 70 of about 1,362 (126)

Room temperature structures beyond 1.5 Å by serial femtosecond crystallography

Structural Dynamics, 2015
About 2.5 × 106 snapshots on microcrystals of photoactive yellow protein (PYP) from a recent serial femtosecond crystallographic (SFX) experiment were reanalyzed to maximum resolution.
Marius Schmidt, Kanupriya Pande, Shibom Basu, Jason Tenboer   +3 more
doaj   +1 more source

Application of Micro-Tubing Reeling System to Serial Femtosecond Crystallography

Photonics
Microcrystal delivery instruments are pivotal to performing serial femtosecond crystallography experiments at the XFEL facilities. We present a novel sample delivery technique based on a micro-tubing reeling system (MRS).
Jihan Kim, Sehan Park, Yunje Cho, Jaehyun Park   +3 more
doaj   +1 more source

Serial crystallography on in vivo grown microcrystals using synchrotron radiation

IUCrJ, 2014
Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized
Cornelius Gati, Gleb Bourenkov, Marco Klinge, Dirk Rehders, Francesco Stellato, Dominik Oberthür, Oleksandr Yefanov, Benjamin P. Sommer, Stefan Mogk, Michael Duszenko, Christian Betzel, Thomas R. Schneider, Henry N. Chapman, Lars Redecke   +13 more
doaj   +1 more source

Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1

Communications Chemistry
Sample consumption for serial femtosecond crystallography with X-ray free electron lasers remains a major limitation preventing broader use in macromolecular crystallography.
Diandra Doppler, Alice Grieco, Domin Koh, Abhik Manna, Adil Ansari, Roberto Alvarez, Konstantinos Karpos, Hung Le, Mukul Sonker, Gihan K. Ketawala, Samira Mahmud, Isabel Quereda-Moraleda, Sayantee Sen, Angel L. Pey, Romain Letrun, Katerina Dörner, Jayanath C. P. Koliyadu, Raphael de Wijn, Johan Bielecki, Huijong Han, Chan Kim, Faisal H. M. Koua, Adam Round, Abhisakh Sarma, Tokushi Sato, Christina Schmidt, Mohammad Vakili, Dmitrii Zabelskii, Richard Bean, Adrian P. Mancuso, Joachim Schulz, Raimund Fromme, Milagros Medina, Thomas D. Grant, Petra Fromme, Richard A. Kirian, Sabine Botha, Jose Manuel Martin-Garcia, Alexandra Ros   +38 more
doaj   +1 more source

Preliminary Serial Femtosecond Crystallography Studies of Myoglobin from Equine Skeletal Muscle

Crystals
Myoglobin (Mb), a heme-containing protein, plays crucial roles in storing and transporting oxygen in muscle cells. Various Mb structures have been extensively determined using conventional cryogenic crystallography, providing valuable information for ...
Jaehyun Park, Sehan Park, Ki Hyun Nam
doaj   +1 more source

Convergent-beam attosecond x-ray crystallography

Structural Dynamics
Sub-ångström spatial resolution of electron density coupled with sub-femtosecond to few-femtosecond temporal resolution is required to directly observe the dynamics of the electronic structure of a molecule after photoinitiation or some other ultrafast ...
Henry N. Chapman, Chufeng Li, Saša Bajt, Mansi Butola, J. Lukas Dresselhaus, Dmitry Egorov, Holger Fleckenstein, Nikolay Ivanov, Antonia Kiene, Bjarne Klopprogge, Viviane Kremling, Philipp Middendorf, Dominik Oberthuer, Mauro Prasciolu, T. Emilie S. Scheer, Janina Sprenger, Jia Chyi Wong, Oleksandr Yefanov, Margarita Zakharova, Wenhui Zhang   +19 more
doaj   +1 more source

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

IUCrJ, 2017
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render
Jose M. Martin-Garcia, Chelsie E. Conrad, Garrett Nelson, Natasha Stander, Nadia A. Zatsepin, James Zook, Lan Zhu, James Geiger, Eugene Chun, David Kissick, Mark C. Hilgart, Craig Ogata, Andrii Ishchenko, Nirupa Nagaratnam, Shatabdi Roy-Chowdhury, Jesse Coe, Ganesh Subramanian, Alexander Schaffer, Daniel James, Gihan Ketwala, Nagarajan Venugopalan, Shenglan Xu, Stephen Corcoran, Dale Ferguson, Uwe Weierstall, John C. H. Spence, Vadim Cherezov, Petra Fromme, Robert F. Fischetti, Wei Liu   +29 more
doaj   +1 more source

Possibilities for serial femtosecond crystallography sample delivery at future light sources

Structural Dynamics, 2015
Serial femtosecond crystallography (SFX) uses X-ray pulses from free-electron laser (FEL) sources that can outrun radiation damage and thereby overcome long-standing limits in the structure determination of macromolecular crystals.
L. M. G. Chavas, L. Gumprecht, H. N. Chapman   +2 more
doaj   +1 more source

Single-molecule imaging with longer X-ray laser pulses

IUCrJ, 2015
During the last five years, serial femtosecond crystallography using X-ray laser pulses has been developed into a powerful technique for determining the atomic structures of protein molecules from micrometre- and sub-micrometre-sized crystals. One of the
Andrew V. Martin, Justine K. Corso, Carl Caleman, Nicusor Timneanu, Harry M. Quiney   +4 more
doaj   +1 more source

Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature

Scientific Reports, 2017
Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane.
Rebecka Andersson, Cecilia Safari, Robert Dods, Eriko Nango, Rie Tanaka, Ayumi Yamashita, Takanori Nakane, Kensuke Tono, Yasumasa Joti, Petra Båth, Elin Dunevall, Robert Bosman, Osamu Nureki, So Iwata, Richard Neutze, Gisela Brändén   +15 more
doaj   +1 more source