Results 41 to 50 of about 1,424 (160)

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nature Communications, 2016
Serial femtosecond crystallography using X-ray free-electron lasers has huge potential for time-resolved structural experiments. Here, the authors present a structure of the light-driven proton pump bacteriorhodopsin using these techniques.
Przemyslaw Nogly, Valerie Panneels, Garrett Nelson, Cornelius Gati, Tetsunari Kimura, Christopher Milne, Despina Milathianaki, Minoru Kubo, Wenting Wu, Chelsie Conrad, Jesse Coe, Richard Bean, Yun Zhao, Petra Båth, Robert Dods, Rajiv Harimoorthy, Kenneth R. Beyerlein, Jan Rheinberger, Daniel James, Daniel DePonte, Chufeng Li, Leonardo Sala, Garth J. Williams, Mark S. Hunter, Jason E. Koglin, Peter Berntsen, Eriko Nango, So Iwata, Henry N. Chapman, Petra Fromme, Matthias Frank, Rafael Abela, Sébastien Boutet, Anton Barty, Thomas A. White, Uwe Weierstall, John Spence, Richard Neutze, Gebhard Schertler, Jörg Standfuss   +39 more
doaj   +1 more source

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography [PDF]

Structure, 2016
X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter
Roessler, Christian G, Agarwal, Rakhi, Allaire, Marc, Alonso-Mori, Roberto, Andi, Babak, Bachega, José FR, Bommer, Martin, Brewster, Aaron S, Browne, Michael C, Chatterjee, Ruchira, Cho, Eunsun, Cohen, Aina E, Cowan, Matthew, Datwani, Sammy, Davidson, Victor L, Defever, Jim, Eaton, Brent, Ellson, Richard, Feng, Yiping, Ghislain, Lucien P, Glownia, James M, Han, Guangye, Hattne, Johan, Hellmich, Julia, Héroux, Annie, Ibrahim, Mohamed, Kern, Jan, Kuczewski, Anthony, Lemke, Henrik T, Liu, Pinghua, Majlof, Lars, McClintock, William M, Myers, Stuart, Nelsen, Silke, Olechno, Joe, Orville, Allen M, Sauter, Nicholas K, Soares, Alexei S, Soltis, S Michael, Song, Heng, Stearns, Richard G, Tran, Rosalie, Tsai, Yingssu, Uervirojnangkoorn, Monarin, Wilmot, Carrie M, Yachandra, Vittal, Yano, Junko, Yukl, Erik T, Zhu, Diling, Zouni, Athina   +49 more
openaire   +4 more sources

The expanding toolkit for structural biology: synchrotrons, X-ray lasers and cryoEM

IUCrJ, 2019
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Stephen P. Muench, Svetlana V. Antonyuk, S. Samar Hasnain   +2 more
doaj   +1 more source

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography [PDF]

Science, 2012
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth of large crystals, in part because a sufficient number of molecules must be present in the lattice for the sample to withstand ...
Boutet S., Lomb L., Williams G. J., Barends T. R. M., Aquila A., Doak R. B., Weierstall U., DePonte D. P., Steinbrener J., Shoeman R. L., Messerschmidt M., Barty A., White T. A., Kassemeyer S., Kirian R. A., Seibert M. M., Montanez P. A., Kenney C., Herbst R., Hart P., Pines J., Haller G., Gruner S. M., Philipp H. T., Tate M. W., Hromalik M., Koerner L. J., Van Bakel N., Morse J., Ghonsalves W., Arnlund D., Bogan M. J., Caleman C., Fromme R., Hampton C. Y., Hunter M. S., Johansson L. C., Katona G., Kupitz C., Liang M., Martin A. V., Nass K., Redecke L., Stellato F., Timneanu N., Wang D., Zatsepin N. A., Schafer D., Defever J., Neutze R., Fromme P., Spence J. C. H., Chapman H. N., Schlichting I.   +53 more
openaire   +6 more sources

Exploring the reaction dynamics of alanine racemase using serial femtosecond crystallography. [PDF]

Sci Rep
Alanine racemase (Alr) catalyzes the pyridoxal 5'-phosphate (PLP)-dependent racemization between L- and D-alanine in bacteria. Owing to the potential interest in targeting Alr for antibacterial drug development, several studies have determined the structures of Alr from different species, proposing models for the reaction mechanism.
Kim J, Park J, Lee K, Chung WK, Nam KH, Cho Y.   +5 more
europepmc   +4 more sources

Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Structural Dynamics, 2015
We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller, A. Marx, S. W. Epp, Y. Zhong, A. Kuo, A. R. Balo, J. Soman, F. Schotte, H. T. Lemke, R. L. Owen, E. F. Pai, A. R. Pearson, J. S. Olson, P. A. Anfinrud, O. P. Ernst, R. J. Dwayne Miller   +15 more
doaj   +1 more source

Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

Nature Communications, 2020
rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved ...
Joyce Woodhouse, Gabriela Nass Kovacs, Nicolas Coquelle, Lucas M. Uriarte, Virgile Adam, Thomas R. M. Barends, Martin Byrdin, Eugenio de la Mora, R. Bruce Doak, Mikolaj Feliks, Martin Field, Franck Fieschi, Virginia Guillon, Stefan Jakobs, Yasumasa Joti, Pauline Macheboeuf, Koji Motomura, Karol Nass, Shigeki Owada, Christopher M. Roome, Cyril Ruckebusch, Giorgio Schirò, Robert L. Shoeman, Michel Thepaut, Tadashi Togashi, Kensuke Tono, Makina Yabashi, Marco Cammarata, Lutz Foucar, Dominique Bourgeois, Michel Sliwa, Jacques-Philippe Colletier, Ilme Schlichting, Martin Weik   +33 more
doaj   +1 more source

Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes

Current Opinion in Structural Biology, 2022
Enzymes with iron-containing active sites play crucial roles in catalysing a myriad of oxidative reactions essential to aerobic life. Defining the three-dimensional structures of iron enzymes in resting, oxy-bound intermediate and substrate-bound states is particularly challenging, not least because of the extreme susceptibility of the Fe(III) and Fe ...
Jonathan A.R. Worrall, Michael A. Hough
openaire   +3 more sources

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Preparation of microcrystals in lipidic cubic phase for serial femtosecond crystallography [PDF]

Nature Protocols, 2014
We have recently established a procedure for serial femtosecond crystallography (SFX) in lipidic cubic phase (LCP) for protein structure determination at X-ray free-electron lasers (XFELs). LCP-SFX uses the gel-like LCP as a matrix for growth and delivery of membrane protein microcrystals for crystallographic data collection.
Wei Liu, Andrii Ishchenko, Vadim Cherezov   +2 more
openaire   +2 more sources