Results 41 to 50 of about 3,847 (182)

Towards pump–probe single-crystal XFEL refinements for small-unit-cell systems

IUCrJ, 2023
Serial femtosecond crystallography for small-unit-cell systems has so far seen very limited application despite obvious scientific possibilities. This is because reliable data reduction has not been available for these challenging systems. In particular,
Lise Joost Støckler, Lennard Krause, Bjarke Svane, Kasper Tolborg, Bo Richter, Seiya Takahashi, Tomoki Fujita, Hidetaka Kasai, Michihiro Sugahara, Ichiro Inoue, Eiji Nishibori, Bo Brummerstedt Iversen   +11 more
doaj   +1 more source

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser. [PDF]

, 2015
G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways.
Barty, Anton, Basu, Shibom, Boutet, Sébastien, Caffrey, Martin, Caro, Lydia N, Carragher, Bridget, Chapman, Henry N, Cherezov, Vadim, Coe, Jesse, Conrad, Chelsie E, de Waal, Parker W, Diederichs, Kay, Dong, Yuhui, Ernst, Oliver P, Fromme, Petra, Fromme, Raimund, Gao, Xiang, Gati, Cornelius, Griffin, Patrick R, Grotjohann, Ingo, Gu, Xin, Gurevich, Vsevolod V, Han, Gye Won, He, Yuanzheng, Howe, Nicole, Hubbell, Wayne L, Ishchenko, Andrii, James, Daniel, Jiang, Hualiang, Jiang, Yi, Kang, Yanyong, Katritch, Vsevolod, Ke, Jiyuan, Kupitz, Christopher, Lee, Regina J, Li, Dianfan, Li, Jun, Lisova, Stella, Liu, Haiguang, Liu, Wei, Ma, Jinming, Melcher, Karsten, Messerschmidt, Marc, Moeller, Arne, Pal, Kuntal, Pascal, Bruce D, Potter, Clinton S, Roy-Chowdhury, Shatabdi, Spence, John CH, Standfuss, Jörg, Stevens, Raymond C, Suino-Powell, Kelly M, Tan, MH Eileen, Tan, Minjia, Van Eps, Ned, Vishnivetskiy, Sergey A, Wang, Dingjie, Wang, Meitian, Weierstall, Uwe, West, Graham M, White, Thomas A, Williams, Garth J, Xu, H Eric, Xu, Qingping, Yang, Huaiyu, Yefanov, Oleksandr, Zatsepin, Nadia A, Zhang, Chenghai, Zhao, Yingming, Zheng, Zhong, Zhi, Xiaoyong, Zhou, X Edward   +71 more
core   +1 more source

Serial Femtosecond Crystallography [PDF]

Synchrotron Radiation News, 2015
Published by Taylor & Francis, Philadelphia, Pa.
openaire   +2 more sources

Entering an era of dynamic structural biology…

BMC Biology, 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
doaj   +1 more source

Serial crystallography is just getting started [PDF]

Structural Dynamics
When serial crystallography at XFELs was first being developed, it was unclear how it should best be used. Early experiments included studying single crystals, or weak or small crystals, or sample delivery methods that over used crystal material or under
Aaron S Brewster, Daniel W Paley, David W Mittan-Moreau, Nicholas K Sauter   +3 more
doaj   +1 more source

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography [PDF]

Science, 2012
Size Matters Less X-ray crystallography is a central research tool for uncovering the structures of proteins and other macromolecules. However, its applicability typically requires growth of large crystals, in part because a sufficient number of molecules must be present in the lattice for the sample to withstand ...
Boutet S., Lomb L., Williams G. J., Barends T. R. M., Aquila A., Doak R. B., Weierstall U., DePonte D. P., Steinbrener J., Shoeman R. L., Messerschmidt M., Barty A., White T. A., Kassemeyer S., Kirian R. A., Seibert M. M., Montanez P. A., Kenney C., Herbst R., Hart P., Pines J., Haller G., Gruner S. M., Philipp H. T., Tate M. W., Hromalik M., Koerner L. J., Van Bakel N., Morse J., Ghonsalves W., Arnlund D., Bogan M. J., Caleman C., Fromme R., Hampton C. Y., Hunter M. S., Johansson L. C., Katona G., Kupitz C., Liang M., Martin A. V., Nass K., Redecke L., Stellato F., Timneanu N., Wang D., Zatsepin N. A., Schafer D., Defever J., Neutze R., Fromme P., Spence J. C. H., Chapman H. N., Schlichting I.   +53 more
openaire   +6 more sources

Cooperative allostery and structural dynamics of streptavidin at cryogenic- and ambient-temperature

Communications Biology, 2022
Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature.
Esra Ayan, Busra Yuksel, Ebru Destan, Fatma Betul Ertem, Gunseli Yildirim, Meryem Eren, Oleksandr M. Yefanov, Anton Barty, Alexandra Tolstikova, Gihan K. Ketawala, Sabine Botha, E. Han Dao, Brandon Hayes, Mengning Liang, Matthew H. Seaberg, Mark S. Hunter, Alexander Batyuk, Valerio Mariani, Zhen Su, Frederic Poitevin, Chun Hong Yoon, Christopher Kupitz, Aina Cohen, Tzanko Doukov, Raymond G. Sierra, Çağdaş Dağ, Hasan DeMirci   +26 more
doaj   +1 more source

Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural Dynamics, 2015
Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy.
Valérie Panneels, Wenting Wu, Ching-Ju Tsai, Przemek Nogly, Jan Rheinberger, Kathrin Jaeger, Gregor Cicchetti, Cornelius Gati, Leonhard M. Kick, Leonardo Sala, Guido Capitani, Chris Milne, Celestino Padeste, Bill Pedrini, Xiao-Dan Li, Jörg Standfuss, Rafael Abela, Gebhard Schertler   +17 more
doaj   +1 more source

Towards the spatial resolution of metalloprotein charge states by detailed modeling of XFEL crystallographic diffraction. [PDF]

, 2020
Oxidation states of individual metal atoms within a metalloprotein can be assigned by examining X-ray absorption edges, which shift to higher energy for progressively more positive valence numbers.
Holton, James M, Kern, Jan, Sauter, Nicholas K, Yano, Junko   +3 more
core   +2 more sources

Continuous Diffraction of Molecules and Disordered Molecular Crystals [PDF]

, 2017
The diffraction pattern of a single non-periodic compact object, such as a molecule, is continuous and is proportional to the square modulus of the Fourier transform of that object. When arrayed in a crystal, the coherent sum of the continuous diffracted
Ayyer, Kartik, Barty, Anton, Chapman, Henry N., Mariani, Valerio, Morgan, Andrew, Oberthuer, Dominik, Pande, Kanupriya, White, Thomas A., Yefanov, Oleksandr M.   +8 more
core   +3 more sources