Results 11 to 20 of about 3,847 (182)

Serial femtosecond crystallography. [PDF]

Nat Rev Methods Primers, 2022
With the advent of X-ray Free Electron Lasers (XFELs), new, high-throughput serial crystallography techniques for macromolecular structure determination have emerged. Serial femtosecond crystallography (SFX) and related methods provide possibilities beyond canonical, single-crystal rotation crystallography by mitigating radiation damage and allowing ...
Barends TRM, Stauch B, Cherezov V, Schlichting I.   +3 more
europepmc   +9 more sources

A novel inert crystal delivery medium for serial femtosecond crystallography [PDF]

IUCrJ, 2015
Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins.
Chelsie E. Conrad, Shibom Basu, Daniel James, Dingjie Wang, Alexander Schaffer, Shatabdi Roy-Chowdhury, Nadia A. Zatsepin, Andrew Aquila, Jesse Coe, Cornelius Gati, Mark S. Hunter, Jason E. Koglin, Christopher Kupitz, Garrett Nelson, Ganesh Subramanian, Thomas A. White, Yun Zhao, James Zook, Sébastien Boutet, Vadim Cherezov, John C. H. Spence, Raimund Fromme, Uwe Weierstall, Petra Fromme   +23 more
doaj   +7 more sources

Chemical crystallography by serial femtosecond X-ray diffraction [PDF]

Nature, 2022
AbstractInorganic–organic hybrid materials represent a large share of newly reported structures, owing to their simple synthetic routes and customizable properties1. This proliferation has led to a characterization bottleneck: many hybrid materials are obligate microcrystals with low symmetry and severe radiation sensitivity, interfering with the ...
Elyse A. Schriber, Daniel W. Paley, Robert Bolotovsky, Daniel J. Rosenberg, Raymond G. Sierra, Andrew Aquila, Derek Mendez, Frédéric Poitevin, Johannes P. Blaschke, Asmit Bhowmick, Ryan P. Kelly, Mark Hunter, Brandon Hayes, Derek C. Popple, Matthew Yeung, Carina Pareja-Rivera, Stella Lisova, Kensuke Tono, Michihiro Sugahara, Shigeki Owada, Tevye Kuykendall, Kaiyuan Yao, P. James Schuck, Diego Solis-Ibarra, Nicholas K. Sauter, Aaron S. Brewster, J. Nathan Hohman   +26 more
openaire   +6 more sources

Lipidic phase membrane protein serial femtosecond crystallography [PDF]

Nature Methods, 2012
X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center ...
Johansson L. C., Arnlund D., White T. A., Katona G., Deponte D. P., Weierstall U., Doak R. B., Shoeman R. L., Lomb L., Malmerberg E., Davidsson J., Nass K., Liang M., Andreasson J., Aquila A., Bajt S., Barthelmess M., Barty A., Bogan M. J., Bostedt C., Bozek J. D., Caleman C., Coffee R., Coppola N., Ekeberg T., Epp S. W., Erk B., Fleckenstein H., Foucar L., Graafsma H., Gumprecht L., Hajdu J., Hampton C. Y., Hartmann R., Hartmann A., Hauser G., Hirsemann H., Holl P., Hunter M. S., Kassemeyer S., Kimmel N., Kirian R. A., Maia F. R. N. C., Marchesini S., Martin A. V., Reich C., Rolles D., Rudek B., Rudenko A., Schlichting I., Schulz J., Seibert M. M., Sierra R. G., Soltau H., Starodub D., Stellato F., Stern S., Strulder L., Timneanu N., Ullrich J., Wahlgren W. Y., Wang X., Weidenspointner G., Wunderer C., Fromme P., Chapman H. N., Spence J. C. H., Neutze R.   +67 more
exaly   +10 more sources

Serial femtosecond crystallography: the first five years [PDF]

IUCrJ, 2015
Protein crystallography using synchrotron radiation sources has had a tremendous impact on biology, having yielded the structures of thousands of proteins and given detailed insight into their mechanisms.
Ilme Schlichting
doaj   +4 more sources

Serial femtosecond crystallography: A revolution in structural biology [PDF]

Archives of Biochemistry and Biophysics, 2016
Macromolecular crystallography at synchrotron sources has proven to be the most influential method within structural biology, producing thousands of structures since its inception. While its utility has been instrumental in progressing our knowledge of structures of molecules, it suffers from limitations such as the need for large, well-diffracting ...
Jose M, Martin-Garcia, Chelsie E, Conrad, Jesse, Coe, Shatabdi, Roy-Chowdhury, Petra, Fromme   +4 more
openaire   +4 more sources

Liquid sample delivery techniques for serial femtosecond crystallography [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2014
X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at ...
Uwe Weierstall
openaire   +4 more sources

An outlook on using serial femtosecond crystallography in drug discovery. [PDF]

Expert Opin Drug Discov, 2019
Introduction: X-ray crystallography has made important contributions to modern drug development but its application to many important drug targets has been extremely challenging. The recent emergence of X-ray free electron lasers (XFELs) and advancements in serial femtosecond crystallography (SFX) have offered new opportunities to overcome limitations ...
Mishin A, Gusach A, Luginina A, Marin E, Borshchevskiy V, Cherezov V.   +5 more
europepmc   +6 more sources

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography [PDF]

Nature Communications, 2014
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from
Weierstall, Uwe, James, Daniel, Wang, Chong, White, Thomas A., Wang, Dingjie, Liu, Wei, Spence, John C. H., Bruce Doak, R., Nelson, Garrett, Fromme, Petra, Fromme, Raimund, Grotjohann, Ingo, Kupitz, Christopher, Zatsepin, Nadia A., Liu, Haiguang, Basu, Shibom, Wacker, Daniel, Won Han, Gye, Katritch, Vsevolod, Boutet, Sébastien, Messerschmidt, Marc, Williams, Garth J., Koglin, Jason E., Marvin Seibert, M., Klinker, Markus, Gati, Cornelius, Shoeman, Robert L., Barty, Anton, Chapman, Henry N., Kirian, Richard A., Beyerlein, Kenneth R., Stevens, Raymond C., Li, Dianfan, Shah, Syed T. A., Howe, Nicole, Caffrey, Martin, Cherezov, Vadim   +36 more
openaire   +8 more sources

Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography [PDF]

Cell, 2015
Angiotensin II type 1 receptor (AT(1)R) is a G protein-coupled receptor that serves as a primary regulator for blood pressure maintenance. Although several anti-hypertensive drugs have been developed as AT(1)R blockers (ARBs), the structural basis for AT(1)R ligand-binding and regulation has remained elusive, mostly due to the difficulties of growing ...
Zhang, Haitao, Unal, Hamiyet, Gati, Cornelius, Han, Gye Won, Liu, Wei, Zatsepin, Nadia A., James, Daniel, Wang, Dingjie, Nelson, Garrett, Weierstall, Uwe, Sawaya, Michael R., Xu, Qingping, Messerschmidt, Marc, Williams, Garth J., Boutet, Sébastien, Yefanov, Oleksandr M., White, Thomas A., Wang, Chong, Ishchenko, Andrii, Tirupula, Kalyan C., Desnoyer, Russell, Coe, Jesse, Conrad, Chelsie E., Fromme, Petra, Stevens, Raymond C., Katritch, Vsevolod, Karnik, Sadashiva S., Cherezov, Vadim   +27 more
openaire   +7 more sources