Results 31 to 40 of about 7,626 (235)

Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources [PDF]

Structural Dynamics
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution.
Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip Johnson, Daniel James, Karol Nass, Antonia Furrer, Pikyee Ma, Steffen Bruenle, Cecilia Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly   +28 more
doaj   +2 more sources

Low-dose fixed-target serial synchrotron crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2017
The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room ...
Owen RL, Axford D, Sherrell DA, Kuo A, Ernst OP, Schulz EC, Miller RJ, Mueller-Werkmeister HM.   +7 more
europepmc   +5 more sources

The HARE chip for efficient time-resolved serial synchrotron crystallography. [PDF]

J Synchrotron Radiat, 2020
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection.
Mehrabi P, Müller-Werkmeister HM, Leimkohl JP, Schikora H, Ninkovic J, Krivokuca S, Andriček L, Epp SW, Sherrell D, Owen RL, Pearson AR, Tellkamp F, Schulz EC, Miller RJD.   +13 more
europepmc   +7 more sources

Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures. [PDF]

Proc Natl Acad Sci U S A, 2020
de la Mora E, Coquelle N, Bury CS, Rosenthal M, Holton JM, Carmichael I, Garman EF, Burghammer M, Colletier JP, Weik M.   +9 more
europepmc   +2 more sources

Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. [PDF]

IUCrJ, 2022
Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus,
Zielinski KA, Prester A, Andaleeb H, Bui S, Yefanov O, Catapano L, Henkel A, Wiedorn MO, Lorbeer O, Crosas E, Meyer J, Mariani V, Domaracky M, White TA, Fleckenstein H, Sarrou I, Werner N, Betzel C, Rohde H, Aepfelbacher M, Chapman HN, Perbandt M, Steiner RA, Oberthuer D.   +23 more
europepmc   +6 more sources

Approach of Serial Crystallography II

Crystals, 2021
Serial crystallography (SX) is an emerging X-ray crystallographic method for determining macromolecule structures. It can address concerns regarding the limitations of data collected by conventional crystallography techniques, which require cryogenic ...
Ki-Hyun Nam
doaj   +1 more source

Serial X-ray Crystallography

Crystals, 2022
Serial crystallography (SX) is an emerging technique to determine macromolecules at room temperature. SX with a pump–probe experiment provides the time-resolved dynamics of target molecules.
Ki Hyun Nam
doaj   +1 more source

Room-temperature serial synchrotron crystallography structure of Spinacia oleracea RuBisCO. [PDF]

Acta Crystallogr F Struct Biol Commun
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the enzyme responsible for the first step of carbon dioxide (CO 2 ) fixation in plants, which proceeds via the carboxylation of ribulose 1,5-biphosphate.
Bjelčić M, Aurelius O, Nan J, Neutze R, Ursby T.   +4 more
europepmc   +4 more sources

A split-beam probe-pump-probe scheme for femtosecond time resolved protein X-ray crystallography [PDF]

, 2015
In order to exploit the femtosecond pulse duration of X-ray Free-Electron Lasers (XFEL) operating in the hard X-ray regime for ultrafast time-resolved protein crystallography experiments, critical parameters that determine the crystallographic signal-to ...
Madsen, A, Van Thor, JJ
core   +4 more sources

Room Temperature Serial Crystallography at Synchrotrons [PDF]

Acta Crystallographica Section A Foundations and Advances, 2014
Serial Femtosecond Crystallography (SFX) is the most commonly used method for the emerging structure determination at X-ray free-electron lasers (FELs). The high peak brilliance of the FEL and the possibility of using femtosecond pulses afford use of nano-to-micron sized crystals in a diffraction-before-destruction approach for the acquisition of high ...
Sabine Botha, Thomas Barends, Wolfgang Kabsch, Beatrice Latz, Karol Nass, Robert Shoeman, Florian Dworkowski, Ezequiel Panepucci, Meitian Wang, Ilme Schlichting, R. Bruce Doak   +10 more
openaire   +1 more source