Results 11 to 20 of about 7,626 (235)

Room-temperature macromolecular serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A new approach for collecting data from many hundreds of thousands of microcrystals using X-ray pulses from a free-electron laser has recently been developed.
Francesco Stellato, Dominik Oberthür, Mengning Liang, Richard Bean, Cornelius Gati, Oleksandr Yefanov, Anton Barty, Anja Burkhardt, Pontus Fischer, Lorenzo Galli, Richard A. Kirian, Jan Meyer, Saravanan Panneerselvam, Chun Hong Yoon, Fedor Chervinskii, Emily Speller, Thomas A. White, Christian Betzel, Alke Meents, Henry N. Chapman   +19 more
doaj   +8 more sources

Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

Crystals, 2022
Serial crystallography (SX) enables the determination of room-temperature structures with minimal radiation damage. The photon flux of the pink beam of 1.2% bandwidth (BW) is one order higher than that of the monochromatic beam from a silicon crystal ...
Yongsam Kim, Ki Hyun Nam
doaj   +4 more sources

Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. [PDF]

Sci Adv, 2021
AbstractFor the two proteins myoglobin (MB) and fluoroacetate dehalogenase (FAcD), we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of crystals, the same sample delivery device, as well as the same
Mehrabi P, Bücker R, Bourenkov G, Ginn HM, von Stetten D, Müller-Werkmeister HM, Kuo A, Morizumi T, Eger BT, Ou WL, Oghbaey S, Sarracini A, Besaw JE, Pare-Labrosse O, Meier S, Schikora H, Tellkamp F, Marx A, Sherrell DA, Axford D, Owen RL, Ernst OP, Pai EF, Schulz EC, Miller RJD.   +24 more
europepmc   +9 more sources

Serial macromolecular crystallography at ALBA Synchrotron Light Source

Journal of Synchrotron Radiation, 2022
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In
Jose M. Martin-Garcia, Sabine Botha, Hao Hu, Rebecca Jernigan, Albert Castellví, Stella Lisova, Fernando Gil, Barbara Calisto, Isidro Crespo, Shatabdi Roy-Chowdhury, Alice Grieco, Gihan Ketawala, Uwe Weierstall, John Spence, Petra Fromme, Nadia Zatsepin, Dirk Roeland Boer, Xavi Carpena   +17 more
doaj   +5 more sources

Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography [PDF]

Science, 2019
AbstractConformational dynamics are essential for proteins to function. Here we describe how we adapted time-resolved serial crystallography developed at X-ray lasers to visualize protein motions using synchrotrons. We recorded the structural changes upon proton pumping in bacteriorhodopsin over 200 ms in time.
Weinert, Tobias, Skopintsev, Petr, James, Daniel, Dworkowski, Florian, Panepucci, Ezequiel, Kekilli, Demet, Furrer, Antonia, Brünle, Steffen, Mous, Sandra, Ozerov, Dmitry, Nogly, Przemyslaw, Wang, Meitian, Standfuss, Jörg   +12 more
openaire   +4 more sources

Serial synchrotron crystallography for time-resolved structural biology

Current Opinion in Structural Biology, 2020
The current state-of-the-art experiments in time-resolved structural biology are undoubtedly the recent extremely impressive results that are emerging from XFEL-based experiments. However, there is a large range of macromolecular systems where the biological interest is predominantly in the slower dynamics (μs-s), that produce well diffracting ...
Pearson, A., Mehrabi, P.
openaire   +5 more sources

Fixed-Target Serial Synchrotron Crystallography Using Nylon Mesh and Enclosed Film-Based Sample Holder

Crystals, 2020
Serial crystallography (SX) technique using synchrotron X-ray allows the visualization of room-temperature crystal structures with low-dose data collection as well as time-resolved molecular dynamics.
Suk-Youl Park, Hyeongju Choi, Cheolsoo Eo, Yunje Cho, Ki Hyun Nam   +4 more
doaj   +3 more sources

Serial macromolecular crystallography at ALBA Synchrotron Light Source. Erratum

Journal of Synchrotron Radiation, 2022
A revised version of Table 2 of Martin-Garcia et al. [J. Synchrotron Rad. (2022). 29, 896–907] is provided.
Jose M. Martin-Garcia, Sabine Botha, Hao Hu, Rebecca Jernigan, Albert Castellví, Stella Lisova, Fernando Gil, Barbara Calisto, Isidro Crespo, Shatabdi Roy-Chowdhury, Alice Grieco, Gihan Ketawala, Uwe Weierstall, John Spence, Petra Fromme, Nadia Zatsepin, Dirk Roeland Boer, Xavi Carpena   +17 more
doaj   +2 more sources

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons [PDF]

Nature Communications, 2017
AbstractHistorically, room-temperature structure determination was succeeded by cryo-crystallography to mitigate radiation damage. Here, we demonstrate that serial millisecond crystallography at a synchrotron beamline equipped with high-viscosity injector and high frame-rate detector allows typical crystallographic experiments to be performed at room ...
Tobias Weinert, Natacha Olieric, Robert Cheng, Steffen Brünle, Daniel James, Dmitry Ozerov, Dardan Gashi, Laura Vera, May Marsh, Kathrin Jaeger, Florian Dworkowski, Ezequiel Panepucci, Shibom Basu, Petr Skopintsev, Andrew S. Doré, Tian Geng, Robert M. Cooke, Mengning Liang, Andrea E. Prota, Valerie Panneels, Przemyslaw Nogly, Ulrich Ermler, Gebhard Schertler, Michael Hennig, Michel O. Steinmetz, Meitian Wang, Jörg Standfuss   +26 more
openaire   +7 more sources

Standard Sample Preparation for Serial Femtosecond Crystallography [PDF]

Biomolecules
The development of serial crystallography (SX), including serial synchrotron crystallography (SSX) at synchrotron sources and serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs), has facilitated the collection of high ...
Christina Schmidt, Kristina Lorenzen, Joachim Schulz, Huijong Han   +3 more
doaj   +2 more sources