Results 11 to 20 of about 3,647 (218)

Best practices for time-resolved serial synchrotron crystallography [PDF]

Acta Crystallographica Section D: Structural Biology, 2022
With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just
Eike C Schulz, Briony A Yorke, Arwen R Pearson   +2 more
exaly   +7 more sources

The HARE chip for efficient time-resolved serial synchrotron crystallography [PDF]

Journal of Synchrotron Radiation, 2020
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection.
Pedram Mehrabi, Henrike M Müller-Werkmeister, Hendrik Schikora   +2 more
exaly   +8 more sources

Low-dose fixed-target serial synchrotron crystallography [PDF]

Acta Crystallographica Section D: Structural Biology, 2017
The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room ...
Robin L Owen, Danny Axford, Darren A Sherrell   +2 more
exaly   +6 more sources

Room-temperature serial synchrotron crystallography of Drosophila cryptochrome [PDF]

Acta Crystallographica Section D: Structural Biology, 2022
Fixed-target serial crystallography allows the high-throughput collection of diffraction data from small crystals at room temperature. This methodology is particularly useful for difficult samples that have sensitivity to radiation damage or intolerance to cryoprotection measures; fixed-target methods also have the added benefit of low sample ...
Abir Ganguly, Brian R Crane
exaly   +4 more sources

Serial macromolecular crystallography at ALBA Synchrotron Light Source

Journal of Synchrotron Radiation, 2022
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In
Jose M. Martin-Garcia, Sabine Botha, Hao Hu, Rebecca Jernigan, Albert Castellví, Stella Lisova, Fernando Gil, Barbara Calisto, Isidro Crespo, Shatabdi Roy-Chowdhury, Alice Grieco, Gihan Ketawala, Uwe Weierstall, John Spence, Petra Fromme, Nadia Zatsepin, Dirk Roeland Boer, Xavi Carpena   +17 more
doaj   +5 more sources

Room-temperature serial synchrotron crystallography structure of Spinacia oleracea RuBisCO [PDF]

Acta Crystallographica Section F, Structural Biology Communications
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the enzyme responsible for the first step of carbon dioxide (CO 2 ) fixation in plants, which proceeds via the carboxylation of ribulose 1,5-biphosphate.
Thomas Ursby, Richard Neutze, Jie Nan
exaly   +5 more sources

Room-temperature macromolecular serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A new approach for collecting data from many hundreds of thousands of microcrystals using X-ray pulses from a free-electron laser has recently been developed.
Francesco Stellato, Dominik Oberthür, Mengning Liang, Richard Bean, Cornelius Gati, Oleksandr Yefanov, Anton Barty, Anja Burkhardt, Pontus Fischer, Lorenzo Galli, Richard A. Kirian, Jan Meyer, Saravanan Panneerselvam, Chun Hong Yoon, Fedor Chervinskii, Emily Speller, Thomas A. White, Christian Betzel, Alke Meents, Henry N. Chapman   +19 more
doaj   +6 more sources

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation [PDF]

IUCrJ, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall   +26 more
doaj   +8 more sources

Serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A brief history is given of how X-ray diffraction data from crystals have been recorded. Today there are new possibilities, spawned by the availability of free electron lasers that produce powerful femtosecond long X-ray pulses.
Michael G. Rossmann
doaj   +3 more sources

Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source

IUCrJ, 2023
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary ...
Filip Leonarski, Jie Nan, Zdenek Matej, Quentin Bertrand, Antonia Furrer, Ishkhan Gorgisyan, Monika Bjelčić, Michal Kepa, Hannah Glover, Viktoria Hinger, Thomas Eriksson, Aleksander Cehovin, Mikel Eguiraun, Piero Gasparotto, Aldo Mozzanica, Tobias Weinert, Ana Gonzalez, Jörg Standfuss, Meitian Wang, Thomas Ursby, Florian Dworkowski   +20 more
doaj   +3 more sources