Results 11 to 20 of about 3,653 (216)

Low-dose fixed-target serial synchrotron crystallography [PDF]

Acta Crystallographica Section D: Structural Biology, 2017
The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room ...
Danny Axford, Henrike Mueller-Werkmeister, Eike C Schulz   +2 more
exaly   +6 more sources

The HARE chip for efficient time-resolved serial synchrotron crystallography [PDF]

Journal of Synchrotron Radiation, 2020
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection.
Sascha W Epp, Arwen Pearson, Henrike Mueller-Werkmeister   +2 more
exaly   +8 more sources

Room-temperature serial synchrotron crystallography of Drosophila cryptochrome [PDF]

Acta Crystallographica Section D: Structural Biology, 2022
Fixed-target serial crystallography allows the high-throughput collection of diffraction data from small crystals at room temperature. This methodology is particularly useful for difficult samples that have sensitivity to radiation damage or intolerance to cryoprotection measures; fixed-target methods also have the added benefit of low sample ...
Brian Crane, Abir Ganguly
exaly   +4 more sources

Room-temperature macromolecular serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A new approach for collecting data from many hundreds of thousands of microcrystals using X-ray pulses from a free-electron laser has recently been developed.
Francesco Stellato, Dominik Oberthür, Mengning Liang, Richard Bean, Cornelius Gati, Oleksandr Yefanov, Anton Barty, Anja Burkhardt, Pontus Fischer, Lorenzo Galli, Richard A. Kirian, Jan Meyer, Saravanan Panneerselvam, Chun Hong Yoon, Fedor Chervinskii, Emily Speller, Thomas A. White, Christian Betzel, Alke Meents, Henry N. Chapman   +19 more
doaj   +6 more sources

Analysis of Multi-Hit Crystals in Serial Synchrotron Crystallography Experiments Using High-Viscosity Injectors

Crystals, 2021
Serial Synchrotron Crystallography (SSX) is rapidly emerging as a promising technique for collecting data for time-resolved structural studies or for performing room temperature micro-crystallography measurements using micro-focused beamlines.
, Marjan Hadian-Jazi, Connie Darmanin
exaly   +3 more sources

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation [PDF]

IUCrJ, 2015
Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly, Daniel James, Dingjie Wang, Thomas A. White, Nadia Zatsepin, Anastasya Shilova, Garrett Nelson, Haiguang Liu, Linda Johansson, Michael Heymann, Kathrin Jaeger, Markus Metz, Cecilia Wickstrand, Wenting Wu, Petra Båth, Peter Berntsen, Dominik Oberthuer, Valerie Panneels, Vadim Cherezov, Henry Chapman, Gebhard Schertler, Richard Neutze, John Spence, Isabel Moraes, Manfred Burghammer, Joerg Standfuss, Uwe Weierstall   +26 more
doaj   +8 more sources

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

IUCrJ, 2017
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render
Robert Fischetti, Nirupa Nagaratnam, Andrii Ishchenko   +2 more
exaly   +3 more sources

Serial crystallography using synchrotron radiation [PDF]

IUCrJ, 2014
A brief history is given of how X-ray diffraction data from crystals have been recorded. Today there are new possibilities, spawned by the availability of free electron lasers that produce powerful femtosecond long X-ray pulses.
Michael G. Rossmann
doaj   +3 more sources

Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

Crystals, 2023
Serial crystallography (SX) enables the determination of the structure of macromolecules or small molecules with minimal radiation damage. In particular, biomolecule structures determined using the SX technique have the advantage of providing room ...
Ki Hyun Nam, Nam Ki Hyun, Yongsam Kim
exaly   +3 more sources

Best practices for time-resolved serial synchrotron crystallography. [PDF]

Acta Crystallogr D Struct Biol, 2022
With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just
Schulz EC, Yorke BA, Pearson AR, Mehrabi P.   +3 more
europepmc   +6 more sources