Results 131 to 140 of about 655,816 (301)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A representation learning-based time series label propagation for smart grid attack detection

Discover Data
The rising incidence of cyber attacks targeting smart grids underscores the urgent need for robust protection mechanisms. Most of the existing smart grid cyber attack detection schemes need correctly labeled data for training.
Smruti P. Dash, Kedar V. Khandeparkar
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Pattern Representation of Gas Concentration Time Series Based on Piecewise Linear Method

Gong-kuang zidonghua, 2010
The paper indicated it not only has low efficiency of data mining to directly use original gas concentration time series to forecast short-term concentration,query similarity and classify and cluster time series,but also affects accuracy and reliability ...
XU Hui~, ZHANG Jian-ying~, CHEN Juan~, ZHAO Zhi-kai~   +3 more
doaj  

Time Series Classification Using Federated Convolutional Neural Networks and Image-Based Representations

IEEE Access
Time series classification (TSC) has numerous applications across various domains. This research introduces a federated hybrid TSC method that combines image-based time series representation techniques with Convolutional Neural Networks (CNNs) in a ...
Felipe A. R. Silva, Omid Orang, Fabricio Javier Erazo-Costa, Petronio C. L. Silva, Pedro H. Barros, Ricardo P. M. Ferreira, Frederico Gadelha Guimaraes   +6 more
doaj   +1 more source

Attention as Robust Representation for Time Series Forecasting

CoRR
Time series forecasting is essential for many practical applications, with the adoption of transformer-based models on the rise due to their impressive performance in NLP and CV. Transformers' key feature, the attention mechanism, dynamically fusing embeddings to enhance data representation, often relegating attention weights to a byproduct role.
Peisong Niu, Tian Zhou 0004, Xue Wang 0010, Liang Sun 0001, Rong Jin 0001   +4 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Random Walks and Non-Linear Paths in Macroeconomic Time Series: Some Evidence and Implications [PDF]

This paper investigates whether the inherent non-stationarity of macroeconomic time series is entirely due to a random walk or also to non-linear components. Applying the numerical tools of the analysis of dynamical systems to long time series for the US,
Franco Bevilacqua, Adriaan van Zon
core