Results 231 to 240 of about 22,430,852 (308)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Time Scale Network: An Efficient Shallow Neural Network For Time Series Data in Biomedical Applications. [PDF]

IEEE J Sel Top Signal Process
Meyer T, Shultz C, Dehak N, Moro-Velázquez L, Irazoqui P.   +4 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Long-Time Scale Simulations Reveal Key Dynamics That Drive the Onset of the N State in the Proteorhodopsin Photocycle. [PDF]

J Phys Chem B
Billings KR, Faramarzi S, Mertz B.
europepmc   +1 more source

Time : December 24 - 31, 2018

TIME Asia
core  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Partial asynchrony of coniferous forest carbon sources and sinks at the intra-annual time scale. [PDF]

Nat Commun
Silvestro R, Mencuccini M, García-Valdés R, Antonucci S, Arzac A, Biondi F, Buttò V, Camarero JJ, Campelo F, Cochard H, Čufar K, Cuny HE, de Luis M, Deslauriers A, Drolet G, Fonti MV, Fonti P, Giovannelli A, Gričar J, Gruber A, Gryc V, Guerrieri R, Güney A, Guo X, Huang JG, Jyske T, Kašpar J, Kirdyanov AV, Klein T, Lemay A, Li X, Liang E, Lintunen A, Liu F, Lombardi F, Ma Q, Mäkinen H, Malik RA, Martinez Del Castillo E, Martinez-Vilalta J, Mayr S, Morin H, Nabais C, Nöjd P, Oberhuber W, Olano JM, Ouimette AP, Paljakka TVS, Peltoniemi M, Peters RL, Ren P, Prislan P, Rathgeber CBK, Sala A, Saracino A, Saulino L, Schiestl-Aalto P, Shishov VV, Stokes A, Sukumar R, Sylvain JD, Tognetti R, Treml V, Urban J, Vavrčík H, Vieira J, von Arx G, Wang Y, Yang B, Zeng Q, Zhang S, Ziaco E, Rossi S.   +72 more
europepmc   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Behavioral Time Scale Synaptic Plasticity (BTSP) endows Hyperdimensional Computing with brain-like information retrieval flexibility

Maass W, Yu C, Wu Y, Wang A.
europepmc   +1 more source