Results 71 to 80 of about 384 (212)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Multidimensional Scaling Visualization Using Parametric Similarity Indices

Entropy, 2015
In this paper, we apply multidimensional scaling (MDS) and parametric similarity indices (PSI) in the analysis of complex systems (CS). Each CS is viewed as a dynamical system, exhibiting an output time-series to be interpreted as a manifestation of its ...
J. A. Tenreiro Machado, António M. Lopes, Alexandra M. Galhano   +2 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Interdisciplinarity as a centripetal force: physics-based methods for complex systems to artificial intelligence

Frontiers in Physics
Analytical tools derived from nonlinear dynamics and dynamical systems theory, such as phase-space reconstruction and Recurrence Quantification Analysis (RQA), provide a powerful framework for investigating complex systems across different scientific ...
Giovanna Zimatore, Giovanna Zimatore, Piercesare Grimaldi, Stavros Hatzopoulos, Piotr Henryk Skarzynski, Piotr Henryk Skarzynski, Piotr Henryk Skarzynski   +6 more
doaj   +1 more source

Process Systems Engineering. A Method of Modeling for Nonlinear Process Dynamics by Chaotic Time-Series Analysis.

KAGAKU KOGAKU RONBUNSHU, 1996
カオス的時系列解析による非線形プロセスのモデリング法を開発した.本手法は, 現時刻近傍の時系列データと類似した過去の時系列データをカオス的時系列解析法により検索し, その挙動から予測値を求める.本法の有効性を検証するため, エチレン装置脱メタン塔の塔底温度制御系に適用した.その結果, 本法は有効であることがわかった.
Hanakuma, Yoshitomo, Nakaya, Kazutoyo, Takeuchi, Takeshi, Sasaki, Takashi, Nakanishi, Eiji   +4 more
openaire   +2 more sources

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Review of the Natural Time Analysis Method and Its Applications

Mathematics
A new concept of time, termed natural time, was introduced in 2001. This new concept reveals unique dynamic features hidden behind time-series originating from complex systems.
Panayiotis A. Varotsos, Efthimios S. Skordas, Nicholas V. Sarlis, Stavros-Richard G. Christopoulos   +3 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source