Results 51 to 60 of about 75,457 (257)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

An Experimental Study on the Effectiveness of the Backward-Facing Step Technique on Small-Scale Horizontal-Axis Wind Turbine Rotor Blades

open access: yesEnergies
The aim of this research work was to explore how modifying the design of small-scale HAWT rotor blades through the backward-facing step technique affects their efficiency under varying wind speeds.
Riad Morina, Yahya Erkan Akansu
doaj   +1 more source

Wind Tunnel Test of the Icing Characteristics of Airfoil Rotating around a Vertical Axis

open access: yesInternational Journal of Rotating Machinery, 2020
To determine the process of icing on the rotating machinery, an icing experiment on a rotating airfoil blade was carried out in this paper. First, an icing wind tunnel was fabricated, and its conditional parameters were calibrated.
Yan Li   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Research on Aerodynamic Performance of Asynchronous-Hybrid Dual-Rotor Vertical-Axis Wind Turbines

open access: yesEnergies
This study analyzes the performance degradation of traditional hybrid wind turbines under high blade-tip-speed ratio conditions and proposes solutions through two-dimensional Computational Fluid Dynamics (CFD) simulations.
Wendong Zhang   +7 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

Aerodynamic Optimization Design of a Supersonic Compressor Rotor with High Pressure Ratio

open access: yesInternational Journal of Aerospace Engineering, 2021
Supersonic compressors have a high wheel speed and operational capability, which facilitate a high stage pressure ratio. However, the strong shock waves in the passage of a supersonic rotor and the interference between shock waves and boundary layers can
Cui Cui, Zhenggui Zhou, Endor Liu
doaj   +1 more source

Evaluation of Digital Technologies for Home‐Based Assessment in People With Amyotrophic Lateral Sclerosis

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Digital technologies hold promise for transforming healthcare by enhancing personalized treatments and offer valuable opportunities to improve patient care. Here, we evaluated several novel, self‐administered, home‐based, digital endpoints for their association with corresponding conventional standard clinical measures (primary) in ...
Arne Mueller   +14 more
wiley   +1 more source

Effect of Pitch Angle and Reynolds Number on Aerodynamic Characteristics of a Small Horizontal Axis Wind Rotor

open access: yesJournal of Applied Fluid Mechanics, 2018
Wind tunnel experiments were conducted on a 2-blade horizontal axis wind rotor to investigate the effect of pitch angle and Reynolds number on aerodynamic characteristics.
J. Y. Zhu, P. Q. Liu
doaj  

Influence of design parameters on the performance of slotted Savonius hydrokinetic turbine

open access: yesResults in Engineering
The main objective of this study is to optimize the performance of slotted Savonius hydrokinetic turbine by investigating the effects of blade profile (semi-circular, elliptical, Benesh, NACA6409), number of blades (2–4), overlap ratio (0.05–0.20), gap ...
Rishabh Kumar, Anuj Kumar
doaj   +1 more source

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