Results 121 to 130 of about 22,922 (227)
FRET and TIRF microscopy for single molecule characterisation of synergistic antimicrobial peptides in artificial bilayers [PDF]
Baird Hannah +2 more
doaj +1 more source
Dual‐color STORM resolves the precise architecture and protein distributions within hippocampal astrocytes at the nanoscale. Identification of a sparse but specific MAGL pool in CA1 astrocytic processes. Astrocytic MAGL is not synapse‐associated, suggesting a synapse‐independent role.
Miklós Zöldi, István Katona
wiley +1 more source
Image contrast restoration in total internal reflection fluorescence microscopy
Blurring is a fundamental constraint in images of an optical imaging system. It appears because of interactions of light with the applied optical system and therefore, deblurring depends on features of the optical imaging system. We applied the iterative
Samaneh Pahlevani +2 more
doaj +1 more source
TGF‐β signaling regulates extracellular vesicle (EV) release in cancer cells by modulating the expression and activity of genes associated with EV biogenesis. The TGF‐β‐induced upregulation of RAB4A expression facilitates fast endosomal recycling, a process that limits the fusion of multivesicular bodies with the plasma membrane and EV secretion. Hence,
Dorival Mendes Rodrigues‐Junior +5 more
wiley +1 more source
Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy
International audienceMreB proteins are actin homologs present in nonspherical bacteria. They assemble into membrane-associated discrete filamentous structures that exhibit different dynamic behaviors along the bacterial sidewalls.
Charlène Cornilleau +7 more
core +1 more source
Interview With Barbara Pearse and the Clathrin Family Tree
The clathrin family tree. Timeline of research on clathrin leading to and generated by Barbara Pearse's discovery, with milestones mapped onto the clathrin triskelion.
Frances M. Brodsky, Margaret S. Robinson
wiley +1 more source
Development of a Photostable pH Biosensor Based on mStayGold
The front cover shows the iterative directed evolution process used to evolve the biosensor. Incubation with CO2 allowed for direct screening of both fluorescence and pH sensitivity in mutant colonies on agar. Our efforts produced serapH1.0, a photostable pH biosensor that is highly fluorescent at physiologically neutral pH (7.4) and dim at acidic pH ...
Michael Chang +7 more
wiley +1 more source
The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis.
Ortiz, Pablo A +4 more
core +2 more sources
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution.
Hauke Winkelmann +4 more
doaj +1 more source
Video of mitochondria moving obtained using LIVE-PAINT on a TIRF microscope
Tom20-101B is stably expressed in the cell, alongside 101A-mNeonGreen facilitating LIVE-PAINT labelling of the mitochondrial protein Tom20. This video was obtained using TIRF microscopy and was recorded with a frame rate of 20 frames per second (fps ...
Gidden, Zoe +1 more
core +2 more sources

