Results 121 to 130 of about 22,922 (227)

A Super‐Resolution Approach for Astrocyte‐Specific Molecular Imaging Reveals the Nanoscale Distribution of Monoacylglycerol Lipase, the Metabolic Node Between Endocannabinoid and Prostaglandin Signaling

open access: yesGlia, Volume 74, Issue 9, September 2026.
Dual‐color STORM resolves the precise architecture and protein distributions within hippocampal astrocytes at the nanoscale. Identification of a sparse but specific MAGL pool in CA1 astrocytic processes. Astrocytic MAGL is not synapse‐associated, suggesting a synapse‐independent role.
Miklós Zöldi, István Katona
wiley   +1 more source

Image contrast restoration in total internal reflection fluorescence microscopy

open access: yesResults in Optics
Blurring is a fundamental constraint in images of an optical imaging system. It appears because of interactions of light with the applied optical system and therefore, deblurring depends on features of the optical imaging system. We applied the iterative
Samaneh Pahlevani   +2 more
doaj   +1 more source

RAB4A acts as a negative feedback regulator of extracellular vesicle secretion during TGF‐β signaling

open access: yesThe FEBS Journal, Volume 293, Issue 17, Page 5340-5362, September 2026.
TGF‐β signaling regulates extracellular vesicle (EV) release in cancer cells by modulating the expression and activity of genes associated with EV biogenesis. The TGF‐β‐induced upregulation of RAB4A expression facilitates fast endosomal recycling, a process that limits the fusion of multivesicular bodies with the plasma membrane and EV secretion. Hence,
Dorival Mendes Rodrigues‐Junior   +5 more
wiley   +1 more source

Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy

open access: yes, 2019
International audienceMreB proteins are actin homologs present in nonspherical bacteria. They assemble into membrane-associated discrete filamentous structures that exhibit different dynamic behaviors along the bacterial sidewalls.
Charlène Cornilleau   +7 more
core   +1 more source

Interview With Barbara Pearse and the Clathrin Family Tree

open access: yesTraffic, Volume 27, Issue 3, September 2026.
The clathrin family tree. Timeline of research on clathrin leading to and generated by Barbara Pearse's discovery, with milestones mapped onto the clathrin triskelion.
Frances M. Brodsky, Margaret S. Robinson
wiley   +1 more source

Development of a Photostable pH Biosensor Based on mStayGold

open access: yesChemBioChem, Volume 27, Issue 16, 27 August 2026.
The front cover shows the iterative directed evolution process used to evolve the biosensor. Incubation with CO2 allowed for direct screening of both fluorescence and pH sensitivity in mutant colonies on agar. Our efforts produced serapH1.0, a photostable pH biosensor that is highly fluorescent at physiologically neutral pH (7.4) and dim at acidic pH ...
Michael Chang   +7 more
wiley   +1 more source

Real-time monitoring of NKCC2 endocytosis by total internal reflection fluorescence (TIRF) microscopy

open access: yes, 2016
The apical Na-K-2Cl cotransporter (NKCC2) mediates NaCl reabsorption by the thick ascending limb (TAL). The amount of NKCC2 at the apical membrane of TAL cells is determined by exocytic delivery, recycling, and endocytosis.
Ortiz, Pablo A   +4 more
core   +2 more sources

Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane

open access: yesNature Communications
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover the functional organization of proteins in the plasma membrane with very high spatial and temporal resolution.
Hauke Winkelmann   +4 more
doaj   +1 more source

Video of mitochondria moving obtained using LIVE-PAINT on a TIRF microscope

open access: yes
Tom20-101B is stably expressed in the cell, alongside 101A-mNeonGreen facilitating LIVE-PAINT labelling of the mitochondrial protein Tom20. This video was obtained using TIRF microscopy and was recorded with a frame rate of 20 frames per second (fps ...
Gidden, Zoe   +1 more
core   +2 more sources

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