Results 101 to 110 of about 22,922 (227)

TIRF images of T lymphoblasts polarized on ICAM-1.

, 2012
Single cell images of T lymphoblasts viewed at ICAM-1 level by TIRF showing co-localization of Gαq expression (green) with LFA-1 and endosomal vesicle markers EEA1 (red) and merge of both colors. There is an absence of detection of Rab11 and Rap1 at TIRF
Lena Svensson (159041), Frances Willenbrock (159048), Paula Stanley (159044), Nancy Hogg (159052)   +3 more
core   +1 more source

Amyloid β alters vascular CaV1.2 channel spatiotemporal properties

The Journal of Physiology, EarlyView.
Abstract figure legend Amyloid‐β1‐42 (Aβ1‐42) triggers a male‐specific signalling cascade influencing CaV1.2 spatiotemporal properties in cerebral vascular smooth muscle. The signalling pathway involves NADPH oxidase (NOX)‐derived reactive oxygen species (ROS) activation of protein kinase C (PKC). Aβ1‐42 can also activate protein kinase A (PKA).
Jade L. Taylor, Miguel Martin‐Aragon Baudel, Eric Pereira da Silva, Johannes W. Hell, Madeline Nieves‐Cintron, Manuel F. Navedo   +5 more
wiley   +1 more source

Gas–liquid mass transfer enhancement in the presence of nanoparticles under slug flow in microreactors

AIChE Journal, Volume 72, Issue 10, October 2026.
Abstract Nanofluids containing nanoparticles represent a promising solvent or reaction system for gas–liquid operations. Herein, the effect of nanoparticle (Al2O3 and SiO2) addition on gas–liquid slug flow pattern and mass transfer enhancement was investigated during CO2 absorption into water in microreactors.
Jie Zong, Jun Yue
wiley   +1 more source

A Time-Identified R-Tree: A Workload-Controllable Dynamic Spatio-Temporal Index Scheme for Streaming Processing

ISPRS International Journal of Geo-Information
Many kinds of spatio-temporal data in our daily lives, such as the trajectory data of moving objects, stream natively. Streaming systems exhibit significant advantages in processing streaming data due to their distributed architecture, high throughput ...
Weichen Peng, Luo Chen, Xue Ouyang, Wei Xiong   +3 more
doaj   +1 more source

Using TIRF microscopy to analyze stimulated and basal state B-cell MHC II clustering in response to ageing and dietary fish oil

, 2023
This research focused on developing an efficient TIRF microscopy approach to evaluate membrane protein organization. More specifically, the data demonstrate that TIRF microscopy can detect changes in ex vivo B-cell MHC II lateral organization using a ...
Melton, Mark T., NC DOCKS at East Carolina University   +1 more
core  

Conformational ensembles of the disordered 4E‐BP2:eIF4E complex restrained by smFRET experiments

Protein Science, Volume 35, Issue 10, October 2026.
Abstract Eukaryotic cap‐dependent translation initiation is regulated by binding of the predominantly folded eukaryotic initiation factor 4E (eIF4E) to the intrinsically disordered eIF4E binding proteins (4E‐BPs). Here, we report full‐length atomistic conformational ensembles generated by IDPConformerGenerator and optimized by X‐EISDv2 workflow for ...
Spencer Smyth, Zi Hao Liu, Thomas E. Tsangaris, Teresa Head‐Gordon, Julie D. Forman‐Kay, Claudiu C. Gradinaru   +5 more
wiley   +1 more source

Comparing single molecule TIRF images from glass-immobilised molecules and from cells.

, 2013
(A) Single-molecule TIRF image of glass-immobilised 13.3 nm DNA rulers, labelled at each end with Atto 647 N. (B) Single-molecule TIRF image of T47D cells labelled with anti-EGFR Affibody-Atto 647 N. Scale bar = 8 µm.
Daniel J. Rolfe (169672), Marisa L. Martin-Fernandez (169677), Sarah R. Needham (305713), Laura C. Zanetti-Domingues (305712), David T. Clarke (169665), Michael Hirsch (285591), Richard Wareham (409123)   +6 more
core   +1 more source

Mutual antagonism between auxin and diacylglycerol acyltransferase 2 regulates high‐temperature‐induced hypocotyl elongation

New Phytologist, Volume 252, Issue 1, Page 222-240, October 2026.
Proposed model for auxin–DGAT2 crosstalk regulating high‐temperature‐induced hypocotyl elongation. Summary Cell elongation is a fundamental process in plant growth and development, and it is significantly affected by temperature changes. While the signaling mechanisms driving high‐temperature‐induced cell elongation have been extensively studied, the ...
Mohinur Khamzaaliyeva, Punita Lalchand, Didier‐Deschamps Ashley, Xue Pan   +3 more
wiley   +1 more source

TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps

Nature Communications, 2019
Engagement of T cell receptors (TCRs) induces the formation of microclusters that mediate the downstream signalling events. Here the authors show, using high resolution TIRF-SIM and live cell imaging, that ZAP70 and LAT are recruited to TCR with distinct
Jason Yi, Lakshmi Balagopalan, Tiffany Nguyen, Katherine M. McIntire, Lawrence E. Samelson   +4 more
doaj   +1 more source

Combined TIRF-AFM Setup: Controlled Quenching of Individual Quantum Dots

, 2006
Eckel R, Walhorn V, Pelargus C, et al. Combined TIRF-AFM Setup: Controlled Quenching of Individual Quantum Dots. In: Proceedings of SPIE. Vol 6092.
Thomas Nann, Pelargus, Christoph, Ros, Robert, Walhorn, Volker ; https://orcid.org/, Eckel, Rainer, Dario Anselmetti, Jörg Martini, Robert Ros, Volker Walhorn, Martini, Jörg, Nann, Th., Christoph Pelargus, Rainer Eckel, Anselmetti, Dario ; https://orcid.org/   +13 more
core   +1 more source