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TIRF-Mikroskopie auf den Kopf gestellt
BIOspektrum, 2011Unter Anwendung der TIRF-Mikroskopie lassen sich auch Strukturen an apikalen Oberflachen polarer Epithelzellen darstellen. Somit kann ein neuer Einblick in den Aufbau dieses charakteristischen Zellpols gewonnen werden, der schliesich zur Aufklarung apikaler Proteintransportprozesse fuhren soll.
Christoph Greb, Ralf Jacob, Anja Schué
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Current Protocols in Microbiology, 2008ABSTRACTTotal internal reflection fluorescence (TIRF) microscopy represents a method of exciting and visualizing fluorophores present in the near‐membrane region of live or fixed cells grown on coverslips. TIRF microscopy is based on the total internal reflection phenomenon that occurs when light passes from a high‐refractive medium (e.g., glass) into ...
Andreea Trache, Gerald A. Meininger
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TIRF Array Biosensor for Environmental Monitoring
2004The field of biosensors has been the subject of an increasing number of books and literature reviews [1–5]. Biosensors consist of two major components: the molecular recognition element and a signal transduction mechanism. The molecular recognition element in biosensor technology takes the form of either a biological molecule (antibodies, enzymes or ...
Kim E. Sapsford, Frances S. Ligler
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Development of a TIRF-FLIM microscope for biomedical applications
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 2007Total internal reflection fluorescence microscopy (TIRFM) is a powerful optical technique to observe fluorescence close to surfaces. Associated with fluorescence lifetime imaging, TIRFM enables to measure contrasts independent of fluorophores concentration and reveal intracellular activity with subwavelength axial resolution.
P. Blandin +6 more
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Quantitative single molecule FRET efficiencies using TIRF microscopy
Faraday Discussions, 2015Förster resonance energy transfer (FRET) microscopy at the single molecule level has the potential to yield information on intra and intermolecular distances within the 2–10 nm range of molecules or molecular complexes that undergo frequent conformation changes.
Hildebrandt, Lasse +2 more
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2013
A Risk Evaluation and Mitigation Strategies (REMS) program is a risk management plan that uses risk minimization strategies beyond approved drug labeling to manage serious risks associated with a medication.
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A Risk Evaluation and Mitigation Strategies (REMS) program is a risk management plan that uses risk minimization strategies beyond approved drug labeling to manage serious risks associated with a medication.
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Bacterial surface swimming states revealed by TIRF microscopy
Soft MatterWe developed a method to determine the tilt angle of a surface-swimming bacterium by maximizing image cross-correlations. This allowed identification of six distinct surface-swimming states in Pseudomonas aeruginosa .
Qiuqian Liu +4 more
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Exploring Biophysics at the Membrane with Single-Molecule TIRF Microscopy
Cellular functions result from the action of heteromeric protein machines that typically exhibit greater utility and dynamism than the sum of their parts. Therefore, the structural composition of these multimeric machines must be determined to delineate their functional attributes, including how information is transduced between the extracellular and ...R Charles, Kissell +3 more
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Quantification of Microtubule-Bundling Activity of MAPs Using TIRF Microscopy
Methods in Molecular Biology, 2023Andrei Smertenko
exaly
Principles and best practices of optimizing a micromirror-based multicolor TIRF microscopy system
Optics Communications, 2023Kaley McCluskey, Nynke Dekker
exaly