Results 121 to 130 of about 177,664 (273)

Glycosylated LGALS3BP is highly secreted by bladder cancer cells and represents a novel urinary disease biomarker

Molecular Oncology, EarlyView.
Urinary LGALS3BP is elevated in bladder cancer patients compared to healthy controls as detected by the 1959 antibody–based ELISA. The antibody shows enhanced reactivity to the high‐mannose glycosylated variant secreted by cancer cells treated with kifunensine (KIF).
Asia Pece, Giulio Lovato, Ilaria Cela, Arianna Mercatelli, Benedetta Ferro, Jussi Nikkola, Sara Pagotto, Tommaso Grottola, Vincenzo De Laurenzi, Rossella Cicchetti, Antonio Marchetti, Luigi Schips, Rossano Lattanzio, Stefano Iacobelli, Emily Capone, Peter Black, Mads Daugaard, Michele Marchioni, Gianluca Sala   +18 more
wiley   +1 more source

A Simple Technique For Three-Dimensional Imaging And Segmentation Of Brain Vasculature U Sing Fast Free-Of-Acrylamide Clearing Tissue In Murine

Cell Journal, 2018
Objective Fast Free-of-Acrylamide Clearing Tissue (FACT) is a recently developed protocol for the whole tissue three-dimensional (3D) imaging. The FACT protocol clears lipids using sodium dodecyl sulfate (SDS) to increase the penetration of light and ...
Arezoo Khoradmehr, Fahime Mazaheri, Morteza Anvari, Amin Tamadon   +3 more
doaj   +1 more source

Reduced vascular leakage correlates with breast carcinoma T regulatory cell infiltration but not with metastatic propensity

Molecular Oncology, EarlyView.
A mouse model for vascular normalization and a human breast cancer cohort were studied to understand the relationship between vascular leakage and tumor immune suppression. For this, endothelial and immune cell RNAseq, staining for vascular function, and immune cell profiling were employed.
Liqun He, Chiara Testini, Neda Hekmati, Altea Bonello, Aglaia Schiza, Emmanuel Nwadozi, Mia Phillipson, Carina Strell, Michael Welsh   +8 more
wiley   +1 more source

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

Kerosene as an alternative to xylene in histopathological tissue processing and staining: An experimental study

Journal of Pharmacy and Bioallied Sciences, 2019
Background: Conventional tissue processing is as old as 100 years and still remains the gold standard. Tissue processing involves many steps, of which one of the important steps is clearing. Xylene is one of the common clearing agents used in laboratory,
Janardhanam Dineshshankar, Manikandan Saranya, Periyasamy Tamilthangam, Jeyaraman Swathiraman, Kumaravel Shanmathee, Ravichandran Preethi   +5 more
doaj   +1 more source

Optimized tissue clearing [PDF]

Nature Methods, 2020
openaire   +2 more sources

Class IIa HDACs forced degradation allows resensitization of oxaliplatin‐resistant FBXW7‐mutated colorectal cancer

Molecular Oncology, EarlyView.
HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto, Nicolò Gualandi, Ylenia Cortolezzis, Raffaella Picco, Monica Colitti, Francesca D'Este, Mariachiara Gani, Wayne W. Hancock, Giovanni Terrosu, Cristina Degrassi, Francesca Agostini, Claudio Brancolini, Luigi E. Xodo, Eros Di Giorgio   +13 more
wiley   +1 more source

Protocol for reproducible EZ clearing and labeling, including optimized steps and quantified fluorescence retention on mouse tissue

STAR Protocols
Summary: Here, we present a protocol for preserving fluorescent markers using tissue clearing and immunolabeling in mouse tissue such as sciatic nerve, gut, and spinal cord.
Ivana Gantar, Jade Nguyen, Dimokratis Karamanlis, Steven Ceto, Samira F. Osterop, Domitille Rajot, Sami El-Boustani, Laura Batti   +7 more
doaj   +1 more source

Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy [PDF]

, 2019
Three-dimensional visualization of tissue structures using optical microscopy facilitates the understanding of biological functions. However, optical microscopy is limited in tissue penetration due to severe light scattering. Recently, a series of tissue-
Guzman, Asja, Kaufman, Laura J., Min, Wei, Shen, Yihui, Shi, Lingyan, Wei, Lu, Wei, Mian, Zhao, Zhilun   +7 more
core   +1 more source

3D Imaging of Cleared Tissues

The FASEB Journal, 2020
Tissue clearing technique enables visualization of opaque organ and tissue in 3‐dimension by turning tissue transparent. Current tissue clearing methods are restricted by limited types of tissue that can be cleared with each individual protocol, which inevitably led to the presence of blind‐spots within whole ...
openaire   +1 more source