Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity [PDF]
Scientific DataResearch on CAR-T cell states is crucial for understanding the mechanisms of immunotherapy. Previous studies in live cells have been primarily limited by phototoxicity, resolution, and throughput, making it difficult to conduct further research and ...
Jie Wang +4 more
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Projective light-sheet microscopy with flexible parameter selection [PDF]
Nature CommunicationsProjection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up.
Bingying Chen +11 more
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Self-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy [PDF]
SensorsAdvancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids.
Pooja Kumari +2 more
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In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy [PDF]
Nature CommunicationsSingle molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution.
Marine Cabillic +14 more
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Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy [PDF]
Scientific ReportsLight sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents.
Alice M. Jacob +13 more
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Extended field-of-view light-sheet microscopy [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a ...
Vettenburg Tom
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Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Navigate: an open-source platform for smart light-sheet microscopy [PDF]
Nature MethodsZach Marin +2 more
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High-resolution open-top axially swept light sheet microscopy
BMC Biology, 2023 Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park +3 more
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