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Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
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eduSPIM: Light Sheet Microscopy in the Museum.
PLoS ONE, 2016 Light sheet microscopy in the museumLight sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
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Light-Sheet Microscopy in Neuroscience [PDF]
Annual Review of Neuroscience, 2019 Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Wenze Li +2 more
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Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity [PDF]
Scientific DataResearch on CAR-T cell states is crucial for understanding the mechanisms of immunotherapy. Previous studies in live cells have been primarily limited by phototoxicity, resolution, and throughput, making it difficult to conduct further research and ...
Jie Wang +4 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry and Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
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Projective light-sheet microscopy with flexible parameter selection [PDF]
Nature CommunicationsProjection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up.
Bingying Chen +11 more
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Light-sheet microscopy: a tutorial [PDF]
Advances in Optics and Photonics, 2018 The authors acknowledge financial support from the Spanish Ministerio de Economía y Competitividad (MINECO) through the “Severo Ochoa” program for Centres of Excellence in R&D (SEV-2015-0522) (BIO2014-59614-JIN, RYC-2015-17935, FIS2016-80455-R [AEI/FEDER, UE]); Fundación Cellex; Generalitat de Catalunya through the CERCA program; Fundació la Marató de ...
Emilio J Gualda, Jordi Andilla
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3D single-molecule super-resolution microscopy with a tilted light sheet
Nature Communications, 2018 Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on ...
Anna-Karin Gustavsson +4 more
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Self-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy [PDF]
SensorsAdvancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids.
Pooja Kumari +2 more
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In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy [PDF]
Nature CommunicationsSingle molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution.
Marine Cabillic +14 more
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