Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis [PDF]
Journal of OphthalmologyThe visualization of tissue architecture has long been constrained by optical absorption and scattering phenomena. Recent advances in optical tissue clearing techniques have revolutionized deep tissue imaging by overcoming these fundamental limitations ...
Shuhuai Meng +6 more
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Optimization of the optical transparency of bones by PACT-based passive tissue clearing [PDF]
Experimental and Molecular Medicine, 2023 Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and
Byung-Ho Jin +9 more
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Pharmaceutics, 2020 Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States ...
Shintaro Fumoto +15 more
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Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies [PDF]
Scientific ReportsComplete tumor removal is crucial for successful surgical oncological outcomes. Targeted fluorescence imaging facilitates differentiation between healthy and cancerous tissue, enabling intraoperative margin assessment.
Ali Fakhrolmobasheri +7 more
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Rapid sonication-assisted whole tissue clearing and immunostaining [PDF]
Scientific ReportsHigh-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering ...
Hoi Pan Harry Cheung +6 more
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In vivo tissue clearing with tartrazine and other dye molecules [PDF]
Communications BiologyTartrazine, along with many other absorbing molecules, has recently emerged as a promising tissue-clearing agent for achieving transient and reversible optical transparency in live animals.
Victoria Crunkleton, Guosong Hong
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T-CLEARE: a pilot community-driven tissue clearing protocol repository [PDF]
Frontiers in Bioengineering and BiotechnologySelecting and implementing a tissue clearing protocol is challenging. Established more than 100 years ago, tissue clearing is still a rapidly evolving field of research.
Kurt R. Weiss +13 more
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3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications [PDF]
Frontiers in Bioengineering and BiotechnologyRecent advancements in tissue clearing and three-dimensional (3D) visualization technologies have enabled subcellular-level examination of entire organs, particularly in complex structures such as the ovary and uterus. Traditional histological approaches
Qiqi Liu +6 more
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A guidebook for DISCO tissue clearing
Molecular Systems Biology, 2021 Histological analysis of biological tissues by mechanical sectioning is significantly time‐consuming and error‐prone due to loss of important information during sample slicing.
Muge Molbay +4 more
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Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice [PDF]
Frontiers in NeuroscienceTissue clearing and 3D imaging have emerged as powerful techniques to assess the cellular and tissue-level architecture of the spinal cord. With the rapidly increasing variety and complexity of optical tissue clearing techniques, there is a critical need
Andrew W. Buxton +5 more
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