Results 1 to 10 of about 89,611 (201)

Optimization of the optical transparency of bones by PACT-based passive tissue clearing [PDF]

Experimental and Molecular Medicine, 2023
Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and
Byung-Ho Jin, Jiwon Woo, Mirae Lee, Seockmo Ku, Hyung Seok Moon, Seung Jun Ryu, Young-Min Hyun, Jeong-Yoon Park, Sung Uk Kuh, Yong Eun Cho   +9 more
doaj   +2 more sources

Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis [PDF]

Journal of Ophthalmology
The visualization of tissue architecture has long been constrained by optical absorption and scattering phenomena. Recent advances in optical tissue clearing techniques have revolutionized deep tissue imaging by overcoming these fundamental limitations ...
Shuhuai Meng, Haiyang Sun, Zhengyi Xu, Yixin Shi, Mingyang Chen, He Cai, Junyu Chen   +6 more
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Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies [PDF]

Scientific Reports
Complete tumor removal is crucial for successful surgical oncological outcomes. Targeted fluorescence imaging facilitates differentiation between healthy and cancerous tissue, enabling intraoperative margin assessment.
Ali Fakhrolmobasheri, Thomas S. Nijboer, Bas Keizers, Rozemarijn M. Kooij, Bert van der Vegt, Max J. H. Witjes, Floris J. Voskuil, Pieter J. van der Zaag   +7 more
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3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications [PDF]

Frontiers in Bioengineering and Biotechnology
Recent advancements in tissue clearing and three-dimensional (3D) visualization technologies have enabled subcellular-level examination of entire organs, particularly in complex structures such as the ovary and uterus. Traditional histological approaches
Qiqi Liu, Zhuo Song, Simin Liu, Zirui Dong, Xi Zheng, Tak Yeung Leung, Xiaoyan Chen   +6 more
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T-CLEARE: a pilot community-driven tissue clearing protocol repository [PDF]

Frontiers in Bioengineering and Biotechnology
Selecting and implementing a tissue clearing protocol is challenging. Established more than 100 years ago, tissue clearing is still a rapidly evolving field of research.
Kurt R. Weiss, Jan Huisken, Neda Khanjani, Vesselina Bakalov, Michelle L. Engle, Michelle C. Krzyzanowski, Tom Madden, Deborah R. Maiese, Justin R. Waterfield, David N. Williams, Lauren Wood, Xin Wu, Carol M. Hamilton, Wayne Huggins   +13 more
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Rapid sonication-assisted whole tissue clearing and immunostaining [PDF]

Scientific Reports
High-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering ...
Hoi Pan Harry Cheung, Marianne Lauwers, Zhengao Wang, Jianfeng Wang, Chengyun Ning, Dai Fei Elmer Ker, Dan Michelle Wang   +6 more
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A pH-Adjustable Tissue Clearing Solution That Preserves Lipid Ultrastructures: Suitable Tissue Clearing Method for DDS Evaluation

Pharmaceutics, 2020
Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States ...
Shintaro Fumoto, Eriko Kinoshita, Keisuke Ohta, Kei-ichiro Nakamura, Tasuku Hirayama, Hideko Nagasawa, Die Hu, Kazuya Okami, Riku Kato, Shojiro Shimokawa, Naho Ohira, Koyo Nishimura, Hirotaka Miyamoto, Takashi Tanaka, Shigeru Kawakami, Koyo Nishida   +15 more
doaj   +3 more sources

Finding the best clearing approach - Towards 3D wide-scale multimodal imaging of aged human brain tissue

NeuroImage, 2022
The accessibility of new wide-scale multimodal imaging techniques led to numerous clearing techniques emerging over the last decade. However, clearing mesoscopic-sized blocks of aged human brain tissue remains an extremely challenging task.
Henriette Rusch, Malte Brammerloh, Jens Stieler, Mandy Sonntag, Siawoosh Mohammadi, Nikolaus Weiskopf, Thomas Arendt, Evgeniya Kirilina, Markus Morawski   +8 more
doaj   +3 more sources

Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice [PDF]

Frontiers in Neuroscience
Tissue clearing and 3D imaging have emerged as powerful techniques to assess the cellular and tissue-level architecture of the spinal cord. With the rapidly increasing variety and complexity of optical tissue clearing techniques, there is a critical need
Andrew W. Buxton, Frank L. Jalufka, Margaret E. Hruska, Jack R. Kubaney, Dylan A. McCreedy, Dylan A. McCreedy   +5 more
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Comparative Analyses of Clearing Efficacies of Tissue Clearing Protocols by Using a Punching Assisted Clarity Analysis

Frontiers in Bioengineering and Biotechnology, 2022
The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization of three-dimensional structures with unprecedented depth and detail.
Jiwon Woo, Jiwon Woo, Jiwon Woo, Jiwon Woo, Eunice Yoojin Lee, Mirae Lee, Mirae Lee, Mirae Lee, Seockmo Ku, Jeong-Yoon Park, Jeong-Yoon Park, Jeong-Yoon Park, Yong Eun Cho, Yong Eun Cho, Yong Eun Cho   +14 more
doaj   +3 more sources