Results 21 to 30 of about 179,247 (290)
Methods and Protocols, 2021 Organic solvent dibenzyl ether (DBE)-based protocols have been widely used in adipose tissue clearing. However, benzyl alcohol/benzyl benzoate (BABB)-based clearing has been shown to offer better transparency in other tissues.
Quyen Vi Luong +4 more
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CLARITY techniques based tissue clearing: types and differences
Folia Morphologica, 2022 CLARITY is a tissue imaging technique that uses hydrogel embedded tissue to remove lipids while maintaining the intactness of protein and tissue fine structure.
Z. Guo, Y. Zheng, Y. Zhang
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NeuroImage, 2022 The accessibility of new wide-scale multimodal imaging techniques led to numerous clearing techniques emerging over the last decade. However, clearing mesoscopic-sized blocks of aged human brain tissue remains an extremely challenging task.
Henriette Rusch +8 more
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Clearing for Deep Tissue Imaging [PDF]
Current Protocols in Cytometry, 2018 AbstractBiologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation of tissues for optical microscopy often involves sectioning to a thickness of 50‐100 µm, the practical limits of light penetration and recovery.
Michael Muntifering +5 more
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Pharmaceutics, 2020 Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States ...
Shintaro Fumoto +15 more
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Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]
, 2016 Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison +12 more
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Frontiers in Bioengineering and Biotechnology, 2022 The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization of three-dimensional structures with unprecedented depth and detail.
Jiwon Woo +14 more
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Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy [PDF]
, 2011 In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering.
Clendenon, Sherry G. +4 more
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Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue [PDF]
Journal of Visualized Experiments, 2018 Adipose tissue plays a central role in energy homeostasis and thermoregulation. It is composed of different types of adipocytes, as well as adipocyte precursors, immune cells, fibroblasts, blood vessels, and nerve projections. Although the molecular control of cell type specification and how these cells interact have been increasingly delineated, a ...
Jingyi, Chi +3 more
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Neuroscience in the third dimension: shedding new light on the brain with tissue clearing
Molecular Brain, 2017 For centuries analyses of tissues have depended on sectioning methods. Recent developments of tissue clearing techniques have now opened a segway from studying tissues in 2 dimensions to 3 dimensions. This particular advantage echoes heavily in the field
Robin J. Vigouroux +2 more
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