Clarifying Tissue Clearing [PDF]
Cell, 2015 Biological specimens are intrinsically three dimensional; however, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by "clearing" the tissue have been ongoing for over a century, there have been a large number of recent innovations.
Richardson, Douglas S. +1 more
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Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures [PDF]
Scientific Reports, 2018 Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size ...
June Hoan Kim +8 more
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Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice [PDF]
Frontiers in NeuroscienceTissue clearing and 3D imaging have emerged as powerful techniques to assess the cellular and tissue-level architecture of the spinal cord. With the rapidly increasing variety and complexity of optical tissue clearing techniques, there is a critical need
Andrew W. Buxton +5 more
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Rapid sonication-assisted whole tissue clearing and immunostaining [PDF]
Scientific ReportsHigh-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering ...
Hoi Pan Harry Cheung +6 more
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HyClear: A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues [PDF]
Cells, 2022 3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs.
S. Soroush Nasseri +3 more
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A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging [PDF]
Experimental and Molecular MedicineThe recent advancements in tissue-clearing techniques have opened new possibilities for non-invasive three-dimensional (3D) volumetric imaging of a wide range of biological specimens.
Kitae Kim +6 more
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Tissue clearing and its applications in neuroscience [PDF]
Nature Reviews Neuroscience, 2020 State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several ...
Hiroki R. Ueda +6 more
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Recent progress in tissue optical clearing [PDF]
Laser & Photonics Reviews, 2013 AbstractTissue optical clearing technique provides a prospective solution for the application of advanced optical methods in life sciences. This paper gives a review of recent developments in tissue optical clearing techniques. The physical, molecular and physiological mechanisms of tissue optical clearing are overviewed and discussed.
Zhu Dan +3 more
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MDISCO: A High-Throughput Tissue-Clearing Protocol for Preservation of Endogenous Fluorescence in Whole Mouse Brains [PDF]
Bio-ProtocolOrganic solvent–based tissue clearing methods are widely used for whole-brain imaging but often compromise endogenous fluorescence. Existing protocols, such as iDISCO and fluorescence-preserving variants, have improved optical transparency but still ...
Madeline Martinez +3 more
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See-Through Technology for Biological Tissue: 3-Dimensional Visualization of Macromolecules [PDF]
International Neurourology Journal, 2016 Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues.
Eunsoo Lee, Hyun Jung Kim, Woong Sun
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