A guidebook for DISCO tissue clearing
Molecular Systems Biology, 2021 Histological analysis of biological tissues by mechanical sectioning is significantly time‐consuming and error‐prone due to loss of important information during sample slicing.
Muge Molbay +4 more
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HyClear: A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues [PDF]
Cells, 2022 3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs.
S. Soroush Nasseri +3 more
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Nature Reviews Methods Primers, 2021 Tissue clearing of gross anatomical samples was first described over a century ago and has only recently found widespread use in the field of microscopy. This renaissance has been driven by the application of modern knowledge of optical physics and chemical engineering to the development of robust and reproducible clearing techniques, the arrival of ...
Douglas S Richardson +2 more
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A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging [PDF]
Experimental and Molecular MedicineThe recent advancements in tissue-clearing techniques have opened new possibilities for non-invasive three-dimensional (3D) volumetric imaging of a wide range of biological specimens.
Kitae Kim +6 more
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MDISCO: A High-Throughput Tissue-Clearing Protocol for Preservation of Endogenous Fluorescence in Whole Mouse Brains [PDF]
Bio-ProtocolOrganic solvent–based tissue clearing methods are widely used for whole-brain imaging but often compromise endogenous fluorescence. Existing protocols, such as iDISCO and fluorescence-preserving variants, have improved optical transparency but still ...
Madeline Martinez +3 more
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Aqueous-based tissue clearing in crustaceans [PDF]
Zoological Letters, 2018 Background Investigation of the internal tissues and organs of a macroscopic organism usually requires destructive processes, such as dissection or sectioning. These processes are inevitably associated with the loss of some spatial information. Recently,
Alu Konno, Shigetoshi Okazaki
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Tissue clearing and its applications in human tissues: A review
ViewIn three‐dimensional (3D) space, an unbiased and systemic view of human specimens between structures and functions is required. However, conventional histological sections from specimens have made only limited progress in exploring intact information ...
Hongcheng Mai, Dan Lu
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Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures [PDF]
Scientific Reports, 2018 Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size ...
June Hoan Kim +8 more
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A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana. [PDF]
PLoS ONE, 2016 Confocal microscopy is widely used to visualize gene expression patterns and developmental processes in plants. However, the imaging of plant tissue can be challenging due to its opacity, which often makes previous immersion in a clearing agent necessary.
Thomas J Musielak +3 more
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Rat perivascular adipose tissue microvasculature revealed by tissue clearing [PDF]
Frontiers in PhysiologyPerivascular adipose tissue (PVAT) surrounds the majority of blood vessels and plays diverse roles in vascular and metabolic functions. The hormonal and lipid exchange functions of PVAT require access to blood vessels.
Stephanie W. Watts +3 more
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