Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures [PDF]
Scientific Reports, 2018 Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size ...
June Hoan Kim +8 more
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HyClear: A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues
Cells, 2022 3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs.
S. Soroush Nasseri +3 more
doaj +4 more sources
, 2022 Recent advances in cardiovascular research have led to a more comprehensive understanding of molecular mechanisms of atherosclerosis. It has become apparent that the disease involves three layers of the arterial wall: the intima, the media, and a ...
Yuanfang Li +23 more
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CLARITY techniques based tissue clearing: types and differences [PDF]
Folia Morphologica, 2022 CLARITY is a tissue imaging technique that uses hydrogel embedded tissue to remove lipids while maintaining the intactness of protein and tissue fine structure.
Z. Guo, Y. Zheng, Y. Zhang
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Tissue clearing and its applications in neuroscience [PDF]
Nature Reviews Neuroscience, 2020 State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals.
Alain Chédotal +25 more
core +9 more sources
Comparative Analyses of Clearing Efficacies of Tissue Clearing Protocols by Using a Punching Assisted Clarity Analysis [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization of three-dimensional structures with unprecedented depth and detail.
Jiwon Woo +14 more
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A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging [PDF]
Experimental and Molecular MedicineThe recent advancements in tissue-clearing techniques have opened new possibilities for non-invasive three-dimensional (3D) volumetric imaging of a wide range of biological specimens.
Kitae Kim +6 more
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Ultrasound-Enhanced Chemical Tissue Clearing of Large Specimens for 3D Microscopy: A Rapid and Accessible Method Using Standard Laboratory Equipment [PDF]
Methods and ProtocolsWhile the recently developed tissue clearing protocols pathoDISCO and activeDISCO significantly accelerate the clearing of large tissue specimens through active chemical dehydration using 2,2-dimethoxypropane, the final step of refractive index (RI ...
Klaus Becker +9 more
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Tissue clearing and its applications in human tissues: A review
ViewIn three‐dimensional (3D) space, an unbiased and systemic view of human specimens between structures and functions is required. However, conventional histological sections from specimens have made only limited progress in exploring intact information ...
Hongcheng Mai, Dan Lu
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MDISCO: A High-Throughput Tissue-Clearing Protocol for Preservation of Endogenous Fluorescence in Whole Mouse Brains [PDF]
Bio-ProtocolOrganic solvent–based tissue clearing methods are widely used for whole-brain imaging but often compromise endogenous fluorescence. Existing protocols, such as iDISCO and fluorescence-preserving variants, have improved optical transparency but still ...
Madeline Martinez +3 more
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