Results 31 to 40 of about 89,661 (241)

LiverClear: A versatile protocol for mouse liver tissue clearing

STAR Protocols, 2022
Summary: Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation,
Laura M. Molina, Yekaterina Krutsenko, Nathaniel E.C. Jenkins, Megan C. Smith, Junyan Tao, Travis B. Wheeler, Simon C. Watkins, Alan M. Watson, Satdarshan P. Monga   +8 more
doaj   +1 more source

Tissue Clearing and Its Application to Bone and Dental Tissues [PDF]

Journal of Dental Research, 2019
Opaqueness of animal tissue can be attributed mostly to light absorption and light scattering. In most noncleared tissue samples, confocal images can be acquired at no more than a 100-µm depth. Tissue-clearing techniques have emerged in recent years in the neuroscience field. Many tissue-clearing methods have been developed, and they all follow similar
D, Jing, Y, Yi, W, Luo, S, Zhang, Q, Yuan, J, Wang, E, Lachika, Z, Zhao, H, Zhao   +8 more
openaire   +2 more sources

Tissue clearing and imaging methods for cardiovascular development

iScience, 2021
Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová, Veronika Olejníčková, Alena Kvasilová, Martina Gregorovičová, David Sedmera   +4 more
doaj   +1 more source

Three-Dimensional Virtual Optical Clearing With Cycle-Consistent Generative Adversarial Network

Frontiers in Physics, 2022
High-throughput deep tissue imaging and chemical tissue clearing protocols have brought out great promotion in biological research. However, due to uneven transparency introduced by tissue anisotropy in imperfectly cleared tissues, fluorescence imaging ...
Jiajia Chen, Jiajia Chen, Jiajia Chen, Zhenhong Du, Zhenhong Du, Ke Si, Ke Si, Ke Si, Ke Si   +8 more
doaj   +1 more source

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Journal of Visualized Experiments, 2020
Hydrophobic tissue clearing methods are easily adjustable, fast, and low-cost procedures that allows for the study of a molecule of interest in unaltered tissue samples. Traditional immunolabeling procedures require cutting the sample into thin sections, which restricts the ability to label and examine intact structures.
Kristin N, Kirchner, Hailong, Li, Adam R, Denton, Steven B, Harrod, Charles F, Mactutus, Rosemarie M, Booze   +5 more
openaire   +3 more sources

Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities

Frontiers in Bioengineering and Biotechnology
IntroductionProtocols for tissue clearing have been established and optimized for the central nervous system. However, significant modifications are required for clearing different tissue types.
Maedeh Makki, Zachary A. Molander, Sergio A. Pineda-Castillo, Devin W. Laurence, Shubhra Singhal, Yasmin Eltwafsha, Gerhard A. Holzapfel, Gerhard A. Holzapfel, Tingting Gu, Chung-Hao Lee   +9 more
doaj   +1 more source

FACT Or PACT: A Comparison Between Free-Acrylamide And Acrylamide-Based Passive Sodium Dodecyl Sulfate Tissue Clearing For Whole Tissue Imaging

Cell Journal, 2019
Major biological processes rely on the spatial organization of cells in complex, highly orchestrated three-dimensional (3D) tissues. Until the recent decade, most of information on spatial neural representation primarily came from microscopic imaging of “
Huimei Wang, Arezoo Khoradmehr, Amin Tamadon   +2 more
doaj   +1 more source

Optical clearing of vaginal tissues in cadavers [PDF]

Therapeutics and Diagnostics in Urology 2018, 2018
A nonsurgical laser procedure is being developed for treatment of female stress urinary incontinence (SUI). Previous studies in porcine vaginal tissues, ex vivo, as well as computer simulations, showed the feasibility of using near-infrared laser energy delivered through a transvaginal contact cooling probe to thermally remodel endopelvic fascia, while
Chun-Hung, Chang, Luke A, Hardy, Michael G, Peters, Dina A, Bastawros, Erinn M, Myers, Michael J, Kennelly, Nathaniel M, Fried   +6 more
openaire   +2 more sources

A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging

BMC Biology, 2022
Background Tissue-clearing techniques have recently been developed to make tissues transparent for three-dimensional (3D) imaging at different scales, including single-cell resolution. However, current tissue-clearing workflows have several disadvantages,
Qing-Hong Shan, Xin-Ya Qin, Nan Zhou, Chuan Huang, Yu Wang, Peng Chen, Jiang-Ning Zhou   +6 more
doaj   +1 more source

Chemical clearing and dehydration of GFP expressing mouse brains.

PLoS ONE, 2012
Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has
Klaus Becker, Nina Jährling, Saiedeh Saghafi, Reto Weiler, Hans-Ulrich Dodt   +4 more
doaj   +1 more source