Results 31 to 40 of about 1,922,235 (275)

LiverClear: A versatile protocol for mouse liver tissue clearing

STAR Protocols, 2022
Summary: Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation,
Laura M. Molina, Yekaterina Krutsenko, Nathaniel E.C. Jenkins, Megan C. Smith, Junyan Tao, Travis B. Wheeler, Simon C. Watkins, Alan M. Watson, Satdarshan P. Monga   +8 more
doaj   +1 more source

SnapShot: Tissue Clearing

Cell, 2017
Tissue clearing has become an important tool for the investigation of biological systems in three dimensions. However, many pioneering techniques were based on serendipitous discoveries. Next-generation clearing methods have been (re)designed with a better understanding of the chemistry and physics required to equalize the refractive index throughout a
Douglas S, Richardson, Jeff W, Lichtman
openaire   +2 more sources

Tissue Clearing and Its Application to Bone and Dental Tissues [PDF]

Journal of Dental Research, 2019
Opaqueness of animal tissue can be attributed mostly to light absorption and light scattering. In most noncleared tissue samples, confocal images can be acquired at no more than a 100-µm depth. Tissue-clearing techniques have emerged in recent years in the neuroscience field. Many tissue-clearing methods have been developed, and they all follow similar
D, Jing, Y, Yi, W, Luo, S, Zhang, Q, Yuan, J, Wang, E, Lachika, Z, Zhao, H, Zhao   +8 more
openaire   +2 more sources

Tissue clearing and imaging methods for cardiovascular development

iScience, 2021
Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová, Veronika Olejníčková, Alena Kvasilová, Martina Gregorovičová, David Sedmera   +4 more
doaj   +1 more source

A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana. [PDF]

PLoS ONE, 2016
Confocal microscopy is widely used to visualize gene expression patterns and developmental processes in plants. However, the imaging of plant tissue can be challenging due to its opacity, which often makes previous immersion in a clearing agent necessary.
Thomas J Musielak, Daniel Slane, Christian Liebig, Martin Bayer   +3 more
doaj   +1 more source

Tissue clearing protocols: an overview of current methods and approaches [PDF]

, 2022
Observation of complex cellular shapes in three-dimensional (3D) tissue context requires the use of thick blocks of tissue samples, which are not transparent. To overcome this optical opacity, a specific set of histological techniques have been developed
Tonchev, Anton B; Medical University of Varna, Pavlov, Pavel; Medical University of Varna, Kalinov, Turgay; Medical University of Varna, Kontny, Andreas; Medical University of Varna, Zlatarov, Alexander; Medical University of Varna   +4 more
core   +4 more sources

Three-Dimensional Virtual Optical Clearing With Cycle-Consistent Generative Adversarial Network

Frontiers in Physics, 2022
High-throughput deep tissue imaging and chemical tissue clearing protocols have brought out great promotion in biological research. However, due to uneven transparency introduced by tissue anisotropy in imperfectly cleared tissues, fluorescence imaging ...
Jiajia Chen, Jiajia Chen, Jiajia Chen, Zhenhong Du, Zhenhong Du, Ke Si, Ke Si, Ke Si, Ke Si   +8 more
doaj   +1 more source

Optical clearing of vaginal tissues in cadavers [PDF]

Therapeutics and Diagnostics in Urology 2018, 2018
A nonsurgical laser procedure is being developed for treatment of female stress urinary incontinence (SUI). Previous studies in porcine vaginal tissues, ex vivo, as well as computer simulations, showed the feasibility of using near-infrared laser energy delivered through a transvaginal contact cooling probe to thermally remodel endopelvic fascia, while
Chun-Hung, Chang, Luke A, Hardy, Michael G, Peters, Dina A, Bastawros, Erinn M, Myers, Michael J, Kennelly, Nathaniel M, Fried   +6 more
openaire   +2 more sources

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Journal of Visualized Experiments, 2020
Hydrophobic tissue clearing methods are easily adjustable, fast, and low-cost procedures that allows for the study of a molecule of interest in unaltered tissue samples. Traditional immunolabeling procedures require cutting the sample into thin sections, which restricts the ability to label and examine intact structures.
Kristin N, Kirchner, Hailong, Li, Adam R, Denton, Steven B, Harrod, Charles F, Mactutus, Rosemarie M, Booze   +5 more
openaire   +3 more sources

Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities

Frontiers in Bioengineering and Biotechnology
IntroductionProtocols for tissue clearing have been established and optimized for the central nervous system. However, significant modifications are required for clearing different tissue types.
Maedeh Makki, Zachary A. Molander, Sergio A. Pineda-Castillo, Devin W. Laurence, Shubhra Singhal, Yasmin Eltwafsha, Gerhard A. Holzapfel, Gerhard A. Holzapfel, Tingting Gu, Chung-Hao Lee   +9 more
doaj   +1 more source