Tissue clearing and imaging methods for cardiovascular development
iScience, 2021 Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová +4 more
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Improved application of the electrophoretic tissue clearing technology, CLARITY, to intact solid organs including brain, pancreas, liver, kidney, lung, and intestine [PDF]
, 2015 Background: Mapping of tissue structure at the cellular, circuit, and organ-wide scale is important for understanding physiological and biological functions.
Kim, Shin +4 more
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Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow [PDF]
, 2017 Bone tissue harbors unique and essential physiological processes, such as hematopoiesis, bone growth, and bone remodeling. To enable visualization of these processes at the cellular level in an intact environment, we developed “Bone CLARITY,” a bone ...
Balani, Deepak H. +8 more
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Serial optical coherence microscopy for label-free volumetric histopathology [PDF]
, 2020 The observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories.
A Azaripour +33 more
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A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana. [PDF]
PLoS ONE, 2016 Confocal microscopy is widely used to visualize gene expression patterns and developmental processes in plants. However, the imaging of plant tissue can be challenging due to its opacity, which often makes previous immersion in a clearing agent necessary.
Thomas J Musielak +3 more
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Three-Dimensional Virtual Optical Clearing With Cycle-Consistent Generative Adversarial Network
Frontiers in Physics, 2022 High-throughput deep tissue imaging and chemical tissue clearing protocols have brought out great promotion in biological research. However, due to uneven transparency introduced by tissue anisotropy in imperfectly cleared tissues, fluorescence imaging ...
Jiajia Chen +8 more
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Optical measurements of rat muscle samples under treatment with ethylene glycol and glucose [PDF]
, 2013 With the objective to study the variation of optical properties of rat muscle during optical clearing, we have performed a set of optical measurements from that kind of tissue. The measurements performed were total transmittance, collimated transmittance,
Carvalho, Maria Inês +3 more
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Optical Clearing of Tissues and Cells [PDF]
Journal of Biomedical Optics, 2008 This PDF file contains the editorial “Special Section Guest Editorial: Optical Clearing of Tissues and Cells” for JBO Vol.
Valery V. Tuchin +3 more
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Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology? [PDF]
, 2017 Mammalian organs comprise a variety of cells that interact with each other and have distinct biological roles. Access to evaluate and perturb intact biological systems at the cellular and molecular levels is essential to fully understand their ...
Challis, Collin +3 more
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Frontiers in Bioengineering and BiotechnologyIntroductionProtocols for tissue clearing have been established and optimized for the central nervous system. However, significant modifications are required for clearing different tissue types.
Maedeh Makki +9 more
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