Results 1 to 10 of about 218,117 (279)
Multicolor two-photon light-sheet microscopy [PDF]
Nature Methods, 2014 International audienceTwo-photon microscopy is the most effective approach for deep-tissue fluorescence cellular imaging; however, its application to high-throughput or high-content imaging is often hampered by low pixel rates, challenging multicolor ...
Emmanuel Beaurepaire +8 more
core +10 more sources
Light sheet microscopy with acoustic sample confinement [PDF]
Nature Communications, 2019 Here, the authors integrate an acoustic trap with a compact light sheet fluorescence microscope to provide contactless sample confinement of biological organisms.
Zhengyi Yang +9 more
doaj +8 more sources
Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity [PDF]
Scientific DataResearch on CAR-T cell states is crucial for understanding the mechanisms of immunotherapy. Previous studies in live cells have been primarily limited by phototoxicity, resolution, and throughput, making it difficult to conduct further research and ...
Jie Wang +4 more
doaj +2 more sources
High-resolution open-top axially swept light sheet microscopy [PDF]
BMC Biology, 2023 Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park +3 more
doaj +2 more sources
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy [PDF]
eLife, 2020 In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes.
Sona Valuchova +8 more
doaj +3 more sources
Technical implementations of light sheet microscopy [PDF]
Microscopy Research and Technique, 2018 Fluorescence-based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in ...
Braeckmans, Kevin +5 more
core +5 more sources
Integrated single- and two-photon light sheet microscopy using accelerating beams [PDF]
Scientific Reports, 2017 We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes.
Peeter Piksarv +9 more
doaj +3 more sources
Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
doaj +6 more sources
Projective light-sheet microscopy with flexible parameter selection [PDF]
Nature CommunicationsProjection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up.
Bingying Chen +11 more
doaj +2 more sources
Self-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy [PDF]
SensorsAdvancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids.
Pooja Kumari +2 more
doaj +2 more sources