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Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
doaj +7 more sources
eduSPIM: Light Sheet Microscopy in the Museum.
PLoS ONE, 2016 Light sheet microscopy in the museumLight sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
doaj +10 more sources
Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry and Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
Peter A. Santi
exaly +4 more sources
Optogenetics and Light-Sheet Microscopy [PDF]
, 2023 AbstractLight-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination
Maddalena L +4 more
europepmc +7 more sources
Light-Sheet Microscopy in Neuroscience [PDF]
Annual Review of Neuroscience, 2019 Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Elizabeth M C Hillman +2 more
exaly +3 more sources
Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity [PDF]
Scientific DataResearch on CAR-T cell states is crucial for understanding the mechanisms of immunotherapy. Previous studies in live cells have been primarily limited by phototoxicity, resolution, and throughput, making it difficult to conduct further research and ...
Jie Wang +4 more
doaj +2 more sources
Projective light-sheet microscopy with flexible parameter selection [PDF]
Nature CommunicationsProjection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up.
Bingying Chen +11 more
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Light-sheet microscopy: a tutorial [PDF]
Advances in Optics and Photonics, 2018 This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical
Omar E Olarte +2 more
exaly +5 more sources
Self-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy [PDF]
SensorsAdvancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids.
Pooja Kumari +2 more
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In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy [PDF]
Nature CommunicationsSingle molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution.
Marine Cabillic +14 more
doaj +2 more sources