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Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
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eduSPIM: Light Sheet Microscopy in the Museum.
PLoS ONE, 2016 Light sheet microscopy in the museumLight sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
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Light-Sheet Microscopy in Neuroscience [PDF]
Annual Review of Neuroscience, 2019 Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Wenze Li +2 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry and Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
exaly +3 more sources
Light-sheet microscopy: a tutorial [PDF]
Advances in Optics and Photonics, 2018 The authors acknowledge financial support from the Spanish Ministerio de Economía y Competitividad (MINECO) through the “Severo Ochoa” program for Centres of Excellence in R&D (SEV-2015-0522) (BIO2014-59614-JIN, RYC-2015-17935, FIS2016-80455-R [AEI/FEDER, UE]); Fundación Cellex; Generalitat de Catalunya through the CERCA program; Fundació la Marató de ...
Emilio J Gualda, Jordi Andilla
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3D single-molecule super-resolution microscopy with a tilted light sheet
Nature Communications, 2018 Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on ...
Anna-Karin Gustavsson +4 more
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Extended field-of-view light-sheet microscopy [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a ...
Vettenburg Tom
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Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Hyperspectral light sheet microscopy [PDF]
Nature Communications, 2015 AbstractTo study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three ...
Wiebke Jahr +4 more
openaire +4 more sources