Results 21 to 30 of about 196,730 (281)

Navigate: an open-source platform for smart light-sheet microscopy. [PDF]

open access: yesNat Methods
Marin Z   +17 more
europepmc   +2 more sources

Hyperspectral light sheet microscopy [PDF]

open access: yesNature Communications, 2015
AbstractTo study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three ...
Wiebke Jahr   +4 more
openaire   +4 more sources

High-resolution open-top axially swept light sheet microscopy

open access: yesBMC Biology, 2023
Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park   +3 more
doaj   +1 more source

Multiple airy beams light-sheet fluorescence microscopy

open access: yesFrontiers in Physics, 2022
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu   +9 more
doaj   +1 more source

Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation

open access: yeseLife, 2020
Cancer clone evolution takes place within tissue ecosystem habitats. But, how exactly tumors arise from a few malignant cells within an intact epithelium is a central, yet unanswered question.
Ashna Alladin   +8 more
doaj   +1 more source

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

open access: yesNanophotonics, 2022
Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan   +18 more
doaj   +1 more source

Light sheet fluorescence microscopy for neuroscience [PDF]

open access: yesJournal of Neuroscience Methods, 2019
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella   +2 more
openaire   +4 more sources

Light sheet fluorescence microscopy of cleared human eyes

open access: yesCommunications Biology, 2023
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche   +6 more
doaj   +1 more source

Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus

open access: yesFrontiers in Cell and Developmental Biology, 2021
Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova   +4 more
doaj   +1 more source

The light-sheet microscopy revolution

open access: yesJournal of Optics, 2018
This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
openaire   +3 more sources

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