Results 21 to 30 of about 841 (209)
The light-sheet microscopy revolution
Journal of Optics, 2018 This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
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ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]
Journal of Innovative Optical Health Sciences, 2013 Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI +4 more
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Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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Acoustic light-sheet microscopy [PDF]
, 2021 Abstract Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light.
S. Wunderl +12 more
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Technical implementations of light sheet microscopy [PDF]
Microscopy Research and Technique, 2018 AbstractFluorescence‐based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in genetic and drug screenings. Among the fluorescence‐based microscopy techniques, light sheet
Zagato Elisa +5 more
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Light sheet microscopy with acoustic sample confinement
Nature Communications, 2019 Here, the authors integrate an acoustic trap with a compact light sheet fluorescence microscope to provide contactless sample confinement of biological organisms.
Zhengyi Yang +9 more
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Journal of Integrative Agriculture, 2017 Cell wall composition in monocotyledonous grasses has been identified as a key area of research for developing better feedstocks for forage and biofuel production.
Christopher Brown +2 more
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Confocal multiview light-sheet microscopy [PDF]
Nature Communications, 2015 AbstractSelective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image ...
de Medeiros, Gustavo +9 more
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