Results 21 to 30 of about 218,117 (279)
Computational hyperspectral light-sheet microscopy
Optics Express, 2022 We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens.
Crombez, Sébastien +3 more
openaire +4 more sources
Hyperspectral light sheet microscopy [PDF]
Nature Communications, 2015 AbstractTo study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three ...
Wiebke Jahr +4 more
openaire +4 more sources
Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
doaj +1 more source
Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
openaire +2 more sources
eLife, 2020 Cancer clone evolution takes place within tissue ecosystem habitats. But, how exactly tumors arise from a few malignant cells within an intact epithelium is a central, yet unanswered question.
Ashna Alladin +8 more
doaj +1 more source
Planar Airy beam light-sheet for two-photon microscopy [PDF]
, 2020 We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro +12 more
core +4 more sources
Confocal multiview light-sheet microscopy [PDF]
Nature Communications, 2015 AbstractSelective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image ...
de Medeiros, Gustavo +9 more
openaire +2 more sources
Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Nanophotonics, 2022 Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan +18 more
doaj +1 more source
Light sheet fluorescence microscopy of cleared human eyes
Communications Biology, 2023 We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche +6 more
doaj +1 more source
Virtual-'light-sheet' single-molecule localisation microscopy enables quantitative optical sectioning for super-resolution imaging. [PDF]
, 2015 Single-molecule super-resolution microscopy allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit.
, +13 more
core +15 more sources