Results 21 to 30 of about 4,952,777 (277)

Multiple airy beams light-sheet fluorescence microscopy

Frontiers in Physics, 2022
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu, Shuangyu Gu, Xianghua Yu, Xianghua Yu, Chen Bai, Junwei Min, Runze Li, Yanlong Yang, Baoli Yao, Baoli Yao   +9 more
doaj   +1 more source

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Nanophotonics, 2022
Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan, Tseng Ming Lun, Vyas Sunil, Hsieh Ting-Yu, Wu Jui-Ching, Chen Shang-Yang, Peng Hsiao-Fang, Su Vin-Cent, Huang Tzu-Ting, Kuo Hsin Yu, Chu Cheng Hung, Chen Mu Ku, Chen Jia-Wern, Chen Yu-Chun, Huang Kuang-Yuh, Kuan Chieh-Hsiung, Shi Xu, Misawa Hiroaki, Tsai Din Ping   +18 more
doaj   +1 more source

Computational hyperspectral light-sheet microscopy

Optics Express, 2022
We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens.
Crombez, Sébastien, Leclerc, Pierre, Ray, Cédric, Ducros, Nicolas   +3 more
openaire   +4 more sources

Light sheet fluorescence microscopy for neuroscience [PDF]

Journal of Neuroscience Methods, 2019
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella, Gunn-Moore, Frank, Dholakia, Kishan   +2 more
openaire   +4 more sources

Light sheet fluorescence microscopy of cleared human eyes

Communications Biology, 2023
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Paques   +6 more
doaj   +1 more source

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]

, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Vettenburg, T.; id_orcid, J. Nylk, K. Dholakia, Nylk, J., C. Mitchell, Vettenburg, T., Gunn-Moore, F. J., T. Vettenburg, Mitchell, C., Dholakia, K., Nylk, J.; id_orcid, F. J. Gunn-Moore   +11 more
core   +1 more source

Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus

Frontiers in Cell and Developmental Biology, 2021
Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova, Helena Chmelova, Anna Vavrova, Zbynek Kozmik, Iryna Kozmikova   +4 more
doaj   +1 more source

3D volume rendering of invertebrates using light-sheet fluorescence microscopy [PDF]

, 2015
Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates.
Gualda Manzano, Emilio José, Moreno, Nuno   +1 more
core   +1 more source

The light-sheet microscopy revolution

Journal of Optics, 2018
This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
openaire   +3 more sources

Light sheet microscopy for histopathology applications [PDF]

Biomedical Engineering Letters, 2019
Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples.
Praveen Kumar Poola, Muhammad Imran Afzal, Youngseung Yoo, Ki Hean Kim, Euiheon Chung   +4 more
openaire   +3 more sources