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A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
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3D volume rendering of invertebrates using light-sheet fluorescence microscopy [PDF]
, 2015 Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates.
Gualda Manzano, Emilio José +1 more
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Acoustic light-sheet microscopy [PDF]
, 2021 Abstract Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light.
S. Wunderl +12 more
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +1 more source
Light sheet microscopy for histopathology applications [PDF]
Biomedical Engineering Letters, 2019 Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples.
Praveen Kumar Poola +4 more
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Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]
Journal of Innovative Optical Health Sciences, 2013 Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI +4 more
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Technical implementations of light sheet microscopy [PDF]
Microscopy Research and Technique, 2018 AbstractFluorescence‐based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in genetic and drug screenings. Among the fluorescence‐based microscopy techniques, light sheet
Zagato Elisa +5 more
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Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array [PDF]
, 2020 Parallelized fluorescence imaging has been a long-standing pursuit that can address the unmet need for a comprehensive three-dimensional (3D) visualization of dynamical biological processes with minimal photodamage.
Lai, TK +7 more
core +1 more source