Results 31 to 40 of about 4,952,777 (277)
A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
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Light sheet microscopy of the gerbil cochlea
Journal of Comparative Neurology, 2020 AbstractLight sheet fluorescence microscopy (LSFM) provides a rapid and complete three‐dimensional image of the cochlea. The method retains anatomical relationships—on a micrometer scale—between internal structures such as hair cells, basilar membrane (BM), and modiolus with external surface structures such as the round and oval windows.
Kendall A. Hutson +4 more
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Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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Light-sheets and smart microscopy, an exciting future is dawning
Communications Biology, 2023 Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
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Acoustic light-sheet microscopy [PDF]
, 2021 Abstract Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light.
S. Wunderl +12 more
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Whole-central nervous system functional imaging in larval Drosophila [PDF]
, 2015 This work was supported by the Howard Hughes Medical Institute.Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS.
Pulver, Stefan +6 more
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
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Bessel Light Sheet Structured Illumination Microscopy
, 2016 Biomedical study researchers using animals to model disease and treatment need fast, deep, noninvasive, and inexpensive multi-channel imaging methods. Traditional fluorescence microscopy meets those criteria to an extent.
Noshirvani Allahabadi, Golchehr
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Deep tissue light-sheet microscopy [PDF]
, 2017 Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo.
Quintas Glasner de Medeiros, Gustavo
core +1 more source