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eduSPIM: Light Sheet Microscopy in the Museum. [PDF]
PLoS ONE, 2016 Light sheet microscopy in the museumLight sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
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Optogenetics and Light-Sheet Microscopy [PDF]
, 2023 AbstractLight-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination
Maddalena L. +4 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light ...
Peter A. Santi
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Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
doaj +6 more sources
, 2014 This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample ...
Weber, M., Mickoleit, M., Huisken, J.
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Deconvolution of light sheet microscopy recordings [PDF]
Scientific Reports, 2019 AbstractWe developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification ...
Klaus Becker +7 more
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Confocal multiview light-sheet microscopy [PDF]
Nature Communications, 2015 AbstractSelective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image ...
de Medeiros, Gustavo +9 more
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Light-sheet fluorescence microscopy for biomedicine [PDF]
, 2023 The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration ...
Poulton, Persephone B.
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Hyperspectral light sheet microscopy [PDF]
Nature Communications, 2015 AbstractTo study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three ...
Wiebke Jahr +4 more
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High-resolution open-top axially swept light sheet microscopy
BMC Biology, 2023 Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park +3 more
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