Results 11 to 20 of about 218,117 (279)

Light-Sheet Microscopy in Neuroscience [PDF]

Annual Review of Neuroscience, 2019
Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Elizabeth M C, Hillman, Venkatakaushik, Voleti, Wenze, Li, Hang, Yu   +3 more
openaire   +4 more sources

Deep and fast live imaging with two-photon scanned light-sheet microscopy [PDF]

Nature Methods, 2011
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large ...
A Edelstein, CJ Engelbrecht, D Beis, David S Koos, FO Fahrbach, H Siedentopf, J Huisken, J Huisken, J Huisken, J Palero, J Vermot, John M Choi, N Ji, N Ji, P Pantazis, PJ Keller, PJ Keller, PJ Scherz, PJ Verveer, R Carriles, R Kopp, RM Williams, S Preibisch, Scott E Fraser, T Pologruto, TA Planchon, Thai V Truong, W Denk, W Supatto, Willy Supatto, WR Zipfel   +30 more
core   +5 more sources

Light-sheet microscopy: a tutorial [PDF]

Advances in Optics and Photonics, 2018
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the
Andilla, Jordi, Gualda, Emilio J., Loza-Alvarez, Pablo, Olarte, Omar E.   +3 more
core   +5 more sources

In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy [PDF]

Nature Communications
Single molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution.
Marine Cabillic, Hisham Forriere, Laetitia Bettarel, Corey Butler, Abdelghani Neuhaus, Ihssane Idrissi, Miguel Edouardo Sambrano-Lopez, Julian Rossbroich, Lucas-Raphael Müller, Jonas Ries, Gianluca Grenci, Virgile Viasnoff, Florian Levet, Jean-Baptiste Sibarita, Rémi Galland   +14 more
doaj   +2 more sources

3D single-molecule super-resolution microscopy with a tilted light sheet

Nature Communications, 2018
Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on ...
Anna-Karin Gustavsson, Petar N. Petrov, Maurice Y. Lee, Yoav Shechtman, W. E. Moerner   +4 more
doaj   +2 more sources

Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy [PDF]

Scientific Reports
Light sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents.
Alice M. Jacob, Anna F. Lindemann, Julia Wagenpfeil, Sergej Geiger, Yannik C. Layer, Babak Salam, Sarah Panahabadi, Darius Kurt, Maximilian W. M. Wintergerst, Frank A. Schildberg, Daniel Kuetting, Ulrike I. Attenberger, Zeinab Abdullah, Alexander M. C. Böhner   +13 more
doaj   +2 more sources

Extended field-of-view light-sheet microscopy [PDF]

EPJ Web of Conferences, 2022
Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a ...
Vettenburg Tom
doaj   +1 more source

Light Sheet Fluorescence Microscopy Using Incoherent Light Detection

Engineering Proceedings, 2023
We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava, Christopher Mann, Jonathan Art, Simon Alford   +3 more
doaj   +1 more source

Acoustic light-sheet microscopy [PDF]

, 2021
AbstractLight-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet microscopy (acoustic LSM), which actively manipulates the light propagation inside a large sample to ...
S. Wunderl, A. Ishijima, E. A. Susaki, Z. Xu, H. Song, H. Zha, T. Azuma, I. Sakuma, H. Fukuoka, E. Okada, H. R. Ueda, S. Takagi, K. Nakagawa   +12 more
openaire   +2 more sources

Light Sheet Fluorescence Microscopy Illuminating Soft Matter

Frontiers in Physics, 2021
Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj   +1 more source