Results 11 to 20 of about 841 (209)
High-resolution open-top axially swept light sheet microscopy
BMC Biology, 2023 Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park +3 more
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Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
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eLife, 2020 Cancer clone evolution takes place within tissue ecosystem habitats. But, how exactly tumors arise from a few malignant cells within an intact epithelium is a central, yet unanswered question.
Ashna Alladin +8 more
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Computational hyperspectral light-sheet microscopy
Optics Express, 2022 We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens.
Crombez, Sébastien +3 more
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Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Nanophotonics, 2022 Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan +18 more
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Light sheet fluorescence microscopy of cleared human eyes
Communications Biology, 2023 We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche +6 more
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Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus
Frontiers in Cell and Developmental Biology, 2021 Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova +4 more
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Light sheet fluorescence microscopy for neuroscience [PDF]
Journal of Neuroscience Methods, 2019 The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
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Deconvolution of light sheet microscopy recordings [PDF]
Scientific Reports, 2019 AbstractWe developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification ...
Klaus Becker +7 more
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A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
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