Results 11 to 20 of about 196,730 (281)
Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Deconvolution of light sheet microscopy recordings [PDF]
Scientific Reports, 2019 AbstractWe developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification ...
Klaus Becker +7 more
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Confocal multiview light-sheet microscopy [PDF]
Nature Communications, 2015 AbstractSelective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image ...
de Medeiros, Gustavo +9 more
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Computational hyperspectral light-sheet microscopy
Optics Express, 2022 We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens.
Crombez, Sébastien +3 more
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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
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Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy [PDF]
Scientific ReportsLight sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents.
Alice M. Jacob +13 more
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Extended field-of-view light-sheet microscopy [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a ...
Vettenburg Tom
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Light-sheet fluorescence microscopy for biomedicine [PDF]
, 2023 The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration ...
Poulton, Persephone B.
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