Results 11 to 20 of about 218,117 (279)
Multicolor two-photon light-sheet microscopy [PDF]
Nature Methods, 2014 International audienceTwo-photon microscopy is the most effective approach for deep-tissue fluorescence cellular imaging; however, its application to high-throughput or high-content imaging is often hampered by low pixel rates, challenging multicolor ...
Emmanuel Beaurepaire +8 more
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Light-Sheet Microscopy in Neuroscience [PDF]
Annual Review of Neuroscience, 2019 Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Elizabeth M C, Hillman +3 more
openaire +4 more sources
Deep and fast live imaging with two-photon scanned light-sheet microscopy [PDF]
Nature Methods, 2011 We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large ...
A Edelstein +30 more
core +5 more sources
Light-sheet microscopy: a tutorial [PDF]
Advances in Optics and Photonics, 2018 This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the
Andilla, Jordi +3 more
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Technical implementations of light sheet microscopy [PDF]
Microscopy Research and Technique, 2018 Fluorescence-based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in ...
Braeckmans, Kevin +5 more
core +5 more sources
Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
doaj +6 more sources
3D single-molecule super-resolution microscopy with a tilted light sheet
Nature Communications, 2018 Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on ...
Anna-Karin Gustavsson +4 more
doaj +2 more sources
Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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Acoustic light-sheet microscopy [PDF]
, 2021 AbstractLight-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet microscopy (acoustic LSM), which actively manipulates the light propagation inside a large sample to ...
S. Wunderl +12 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj +1 more source