Results 31 to 40 of about 218,117 (279)
Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus
Frontiers in Cell and Developmental Biology, 2021 Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova +4 more
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Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]
, 2014 The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K. +4 more
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Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes. [PDF]
, 2016 The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'.
Bakowski, Malina A +6 more
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A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
openaire +2 more sources
eduSPIM: Light Sheet Microscopy in the Museum.
PLoS ONE, 2016 Light sheet microscopy in the museumLight sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
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The light-sheet microscopy revolution
Journal of Optics, 2018 This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
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Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +1 more source
Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]
, 2016 Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison +12 more
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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
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