Results 51 to 60 of about 218,117 (279)
Light sheet microscopy v1 [PDF]
, 2019 Protocol documents how to operate light sheet microscope (UltraMicroscope II, LaVision BioTec)
Clara Huesing +3 more
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Vertically scanned laser sheet microscopy [PDF]
, 2014 Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms.
Alicia Arranz +8 more
core +2 more sources
Light sheet microscopy for single molecule tracking in living tissue. [PDF]
PLoS ONE, 2010 Single molecule observation in cells and tissue allows the analysis of physiological processes with molecular detail, but it still represents a major methodological challenge.
Jörg Gerhard Ritter +4 more
doaj +1 more source
Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
openaire +4 more sources
Protocol for the Design and Assembly of a Light Sheet Light Field Microscope
Methods and Protocols, 2019 Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of
Jorge Madrid-Wolff, Manu Forero-Shelton
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Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. [PDF]
PLoS ONE, 2018 Light sheet fluorescence microscopy enables fast, minimally phototoxic, three-dimensional imaging of live specimens, but is currently limited by low throughput and tedious sample preparation.
Savannah L Logan +5 more
doaj +1 more source
Diffractive oblique plane microscopy [PDF]
, 2019 Imaging of neuronal activity with fluorescent indicators is an important technique in neuroscience. However, it remains challenging to record volumetric image data at fast frame rates and good resolution.
Hoffmann, Maximilian +1 more
core +1 more source
Open-top axially swept light sheet microscopy [PDF]
Biomedical Optics Express, 2020 AbstractOpen-top light sheet microscopy (OT-LSM) is a specialized microscopy for the high throughput cellular imaging of large tissues including optically cleared tissues by having all the optical setup below the sample stage. Current OT-LSM systems had relatively low axial resolutions by using weakly focused light sheets to cover the imaging field of ...
Bumju Kim +7 more
openaire +3 more sources
High-speed imaging of amoeboid movements using light-sheet microscopy. [PDF]
PLoS ONE, 2012 Light-sheet microscopy has been developed as a powerful tool for live imaging in biological studies. The efficient illumination of specimens using light-sheet microscopy makes it highly amenable to high-speed imaging. We therefore applied this technology
Daisuke Takao +4 more
doaj +1 more source
Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells [PDF]
, 2013 Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence microscopy method allowing ...
Basu, Srinjan +7 more
core +1 more source