Results 21 to 30 of about 73,867 (250)
Navigate: an open-source platform for smart light-sheet microscopy. [PDF]
Nat MethodsMarin Z +17 more
europepmc +2 more sources
Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Nanophotonics, 2022 Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan +18 more
doaj +1 more source
Light sheet fluorescence microscopy of cleared human eyes
Communications Biology, 2023 We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche +6 more
doaj +1 more source
Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus
Frontiers in Cell and Developmental Biology, 2021 Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova +4 more
doaj +1 more source
Light sheet fluorescence microscopy for neuroscience [PDF]
Journal of Neuroscience Methods, 2019 The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
openaire +4 more sources
Deconvolution of light sheet microscopy recordings [PDF]
Scientific Reports, 2019 AbstractWe developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification ...
Klaus Becker +7 more
openaire +2 more sources
A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
doaj +1 more source
The light-sheet microscopy revolution
Journal of Optics, 2018 This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging.
J M Girkin, M T Carvalho
openaire +3 more sources
Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]
PLoS ONE, 2014 We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan +2 more
doaj +1 more source
Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]
PLoS Computational Biology, 2018 Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert +4 more
doaj +1 more source