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A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues

open access: yesCell Reports, 2020
Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen   +17 more
doaj   +1 more source

Light-sheets and smart microscopy, an exciting future is dawning

open access: yesCommunications Biology, 2023
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj   +1 more source

Whole-central nervous system functional imaging in larval Drosophila [PDF]

open access: yes, 2015
This work was supported by the Howard Hughes Medical Institute.Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS.
Pulver, Stefan   +6 more
core   +1 more source

Applications of Light-Sheet Microscopy in Microdevices

open access: yesFrontiers in Neuroanatomy, 2019
Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet   +11 more
doaj   +1 more source

Three dimensional fluorescence imaging using multiple light-sheet microscopy. [PDF]

open access: yesPLoS ONE, 2014
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system
Kavya Mohan   +2 more
doaj   +1 more source

Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy. [PDF]

open access: yesPLoS Computational Biology, 2018
Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 ...
Martin Weigert   +4 more
doaj   +1 more source

Bessel Light Sheet Structured Illumination Microscopy

open access: yes, 2016
Biomedical study researchers using animals to model disease and treatment need fast, deep, noninvasive, and inexpensive multi-channel imaging methods. Traditional fluorescence microscopy meets those criteria to an extent.
Noshirvani Allahabadi, Golchehr
core   +5 more sources

ADVANCED OPTICAL TECHNIQUES TO EXPLORE BRAIN STRUCTURE AND FUNCTION [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2013
Understanding brain structure and function, and the complex relationships between them, is one of the grand challenges of contemporary sciences. Thanks to their flexibility, optical techniques could be the key to explore this complex network.
L. SILVESTRI   +4 more
doaj   +1 more source

Deep tissue light-sheet microscopy [PDF]

open access: yes, 2017
Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo.
Quintas Glasner de Medeiros, Gustavo
core   +1 more source

Light Sheet Reflectance Microscopy

open access: yes, 2019
Many human diseases are diagnosed by the histopathological analysis of suspicious lesions where cellular morphological changes are examined at microscopic resolution.
Nguyen, Christopher David
core   +4 more sources

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