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Clearing Brain Tissue with Anethole
Stain Technology, 1948Groat (1941) called attention to the necessity of matching the refractive index of the tissue and clearer to obtain maximum transparency of in toto cleared specimens. Tissues that are refractively almost homogeneous, such as muscle, can be cleared after bleaching and dehydrating by placing in an oil of similar refractive index.
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Optical clearing of vaginal tissues
SPIE Proceedings, 2017Near-IR laser energy in conjunction with applied tissue cooling is being investigated for thermal remodeling of endopelvic fascia during minimally invasive treatment of female stress urinary incontinence. Previous simulations of light transport, heat transfer, and tissue thermal damage have shown that a transvaginal approach is more feasible than a ...
Chun-Hung Chang +3 more
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Imaging cleared tissues made easy
Nature Methods, 2022Shigeaki Kanatani, Per Uhlén
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Quantitative Cleared Tissue Imaging
Microscopy and Microanalysis, 2023Hazel Borges +3 more
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Optical Clearing and Tissue Imaging
2019Imaging methods are a powerful tool for diagnostic purposes. In this chapter, the most important light-imaging methods, their advantages, and drawbacks are described. The advantages of radiation-free light-based imaging methods relative to traditional radiation methods, such as X-ray, magnetic resonance, or positron emission imaging, are indicated, and
Luís Manuel Couto Oliveira +1 more
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Optical Clearing of Fibrous Tissues
2010Normally, eye sclera is a turbid medium that is nontransparent in the visible range (see Fig. 7). The origin of scleral spectra formation can be understood on the basis of light scattering by a system of polydispersive irregularly arranged collagen cylinders immersed in the ground substance with a lower refractive index and strong absorption bands ...
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Optical Clearing of Gastric Tissue
2010Transmittance and diffuse reflectance measurements were recently performed over a range from 800 to 2200 nm for previously frozen and fresh native porcine stomach cardiac and pyloric mucosa sections of 1.2-1.6 mm thickness. Mucosa consists of moist epithelium and the connective tissue immediately beneath it. Mucosal structure is somewhat identical to
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KneEZ Clear, an Effective Tissue Clearing Protocol to Study Musculoskeletal Tissues in the Mouse
Wholemount, 3-dimensional (3D) tissue imaging holds significant promise for analyzing heterogeneous musculoskeletal tissues, such as knee joints, that demand time- and labor-intensive processing using traditional histological methods. Current musculoskeletal clearing protocols rely on either solvent-based tissue clearing, which substantially alters theYounis, Julia +12 more
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