Results 101 to 110 of about 405,445 (304)

The genetic applications of plant cell and tissue culture techniques: Essential tools for genetic manipulation and crop improvement

Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Plant cell and tissue culture techniques are fundamental to contemporary plant science, providing essential tools for genetic manipulation and crop improvement. This review examines the primary applications of these techniques, emphasizing their role in
Uthman B. ALGOPISHI, Ahmed M. ABDELGHFFAR, Nora M. AL ABOUD, Fatmah A. SAFHI, Eman FAYAD, Khadiga ALHARBI, Nada I. ALJWAIZEA, Abdallah A. HASSANIN   +7 more
doaj   +1 more source

Plant Tissue Culture : Techniques and Experiments

, 2000
xiv,231 hal,;ill,;21 ...
Smith, Roberta H.,, Smith,Robert H
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Design criteria and performance parameters of an alpha irradiation device for cell studies [PDF]

, 1989
An alpha irradiation device is described that utilises a commercially available disc-shaped americium-241 source of 8 cm diameter. The alpha particles traverse a moving collimator and the source is rotated to reduce the influence of source ...
Kellerer, Albrecht M., Roos, H.
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The selection and application of design methodologies for the design of bone tissue scaffolds [PDF]

, 2013
Research motivation: Bone tissue scaffolds offer a way forward in a strategy to change from tissue replacement to tissue regeneration. Bone tissue scaffolds are a combination of a physical construct, with clearly defined three-dimensional spatial ...
Blogg, Ken
core  

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Study on human chondrocyte culture viability for autologous transplantation in clinical application [PDF]

Einstein (São Paulo), 2003
Objective: The limited regenerative capacity of the cartilage tissuemakes the treatment of chondral lesions difficult. The techniquescurrently available to treat cartilage lesions may relieve symptoms,but do not regenerate the injured tissue.
Christiane Lombello, Geraldo Marins dos Reis Jr, Moisés Cohen   +2 more
doaj  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Fabrication and characterisation of 3 dimensional scaffold for tissue engineering application via microstereolithography technique [PDF]

Microstereolithography is a method used for rapid prototyping of polymeric and ceramic components. This technique converts a computer-aided design (CAD) to a three dimensional (3D) model, and enables layer-per-layer fabrication curing a liquid resin ...
Talib, Marina
core  

Tissue engineering of a tracheal substitute [PDF]

, 2004
Lectin histochemistry and scanning electron microscopy (SEM) was used to assess the growth and characterise the differentiation of human respiratory epithelial cells (REC) cultured on two biomaterial scaffolds.
Ziegelaar, Brian
core