YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Endpoint or Kinetic Measurement of Hydrogen Sulfide Production Capacity in Tissue Extracts. [PDF]
Bio Protoc, 2017 Hine C, Mitchell JR.
europepmc +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Development of amide-based fluorescent probes for selective measurement of carboxylesterase 1 activity in tissue extracts. [PDF]
Anal Biochem, 2017 Kodani SD +4 more
europepmc +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
SIDE COMPARATION OF TWO METHODS FOR QUANTIFYING MALONDIALDEHYDE LEVELS IN ANIMAL TISSUE EXTRACTS
, 2020 Malondialdehyde (MDA) as an important marker used for the assessment of the oxidative level in a tissue of biological fluid. The standard assay method uses thiobarbituric acid for spectrophotometric detection but suffers from a lack of specificity.
Roxana-Nicoleta, Vaideș-Negustor +1 more
core
900MHz 1H-/13C-NMR analysis of 2-hydroxyglutarate and other brain metabolites in human brain tumor tissue extracts. [PDF]
PLoS One, 2018 Park GHJ, Yang SH, Baek HM.
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
FEBS Open Bio, EarlyView.Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes. [PDF]
J Vis Exp, 2018 Yamamoto H, Sawaguchi Y, Kimura M.
europepmc +1 more source