Results 161 to 170 of about 2,550,164 (211)
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Protoheme extraction from plant tissue
Analytical Biochemistry, 1978A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
L C, Stillman, M L, Gassman
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Radioimmunological Determination of Cholecystokinin in Tissue Extracts
Digestion, 2009A sensitive and precise radioimmunoassay has been developed. Cholecystokinin (CCK) was labeled by the Bolton-Hunter method. The antibody bound CCK<sub>8</sub> and CCK<sub>33</sub>. The binding sites of the antibody are located by the sulfated tyrosil group in position 27 of the CCK molecule. Human duodenum tissue contains larger
A, Schafmayer, M, Werner, H D, Becker
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Extraction of Acetylcholine from Brain Tissue
Nature, 1953IN some recent attempts1 to follow rapid changes in the concentration of acetylcholine in mammalian brain in vivo, the experimental animals were killed by freezing in liquid air, a method which had already yielded valuable results when applied to the study of certain other labile metabolites2.
J, CROSSLAND, A J, MERRICK
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Protease Activity in Synovial Tissue Extracts
Scandinavian Journal of Rheumatology, 1972Extracts from synovial membranes obtained from 10 patients with rheumatoid arthritis and 8 patients with non-rheumatoid arthritis were examined for protease activity at different pH on casein, haemoglobin and albumin. At least two different activities are demonstrable.
R B, Andersen, J, Gormsen, P H, Petersen
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Mycoplasmacidal Action of Normal Tissue Extracts
Nature, 1969WHILE studying experimental infection with various mycoplasmas, we found that concentrated homogenates of infected animal tissues sometimes yielded negative cultures, while higher dilutions of the same homogenates were positive. Further examination revealed the presence of a mycoplasmacidal factor in extracts of normal tissues, which appears during ...
E, Kaklamanis +4 more
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Membrane Extracts from Plant Tissues
2020The comparison of isolated plant cell membranous enclosures can be hampered if their extraction method differs, e.g., in regard to the utilized buffers, the tissue, or the developmental stage of the plant. Thus, for comparable results, different cellular compartments should be isolated synchronously in one procedure.
Uwe, Bodensohn +3 more
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Estimation of the blood contamination of tissue extracts
Analytical Biochemistry, 1983A method based on measurement of absorbance at 540 or 580 nm is described. The results obtained were closely correlated to the actual level of blood present in different tissues, as determined by use of radiolabeled albumin. The present technique is much simpler than earlier methods used to determine the degree of blood contamination of tissue extracts.
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Glycogenolytic effect of adipose tissue extract
Experientia, 1975L'administration i.v. d'un extrait aqueux de tissu adipeux (ATE) a des rats intacts surrenalectomises provoque un etat de glycogenolyse hepatique suivi d'hypoglycemie. La glycogenolyse semble etre independante de l'epinephrine. L'hypoglycemie provoquee par l'ATE resulte en partie de la mobilisation acceleree des reserves de glycogene hepatique.
B W, Siegel, T R, Blohm, N L, Wiech
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Simultaneous Extraction of Tissue Norepinephrine and Serotonin
Journal of Pharmaceutical Sciences, 1969A new procedure for the extraction of tissue monoamines combines the homogenization and butanol extraction steps. In addition to the significant reduction in the time necessary for extraction which is characteristic of single-extraction procedures, greater extraction efficiencies are obtained.
B M, Phillips +3 more
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Extraction and quantification of daunomycin and doxorubicin in tissues
Journal of Chromatography B: Biomedical Sciences and Applications, 1980The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection.
J F, Strauss +3 more
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