Results 181 to 190 of about 1,431,832 (205)

Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins

Journal of Biotechnology, 2012
The tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins. The difficulty in obtaining this enzyme led us to look for an optimal method for its use. In this work, we produced both the wild-type and the S219V mutant TEV proteases fused to the Streptag II affinity sequence (Streptag II-TEV(WT), and ...
Miladi, Baligh, Marjou, Ahmed El, Boeuf, Guilhem, Bouallagui, Hassib, Dufour, Florence, Di Martino, Patrick, Elm'Selmi, Abdellatif   +6 more
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Detection and cell-free translation of subgenomic RNAs of tobacco etch virus

Virology, 1983
RNA extracted from purified tobacco etch virus and virus-infected tissue was examined by the northern hybridization technique and found to contain several subgenomic-sized viral RNA species in addition to the 3 x 10(6) MW genomic RNA. Of the 10 subgenomic-sized RNAs detected (2.03, 1.24, 1.14, 1.01, 0.84, 0.62, 0.55, 0.46, 0.34, and 0.28 x 10(6) MW), 4
T, Otal, V, Hari
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Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase

Virology, 1989
The 49-kDa proteinase of tobacco etch virus (TEV) cleaves the polyprotein derived from the TEV genomic RNA at five locations. Molecular genetic and biochemical analyses of the 49-kDa TEV proteinase were performed to test its homology to the cellular trypsin-like serine proteases.
W G, Dougherty, T D, Parks, S M, Cary, J F, Bazan, R J, Fletterick   +4 more
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Multiple Levels of Resistance to Tobacco Etch Virus in Pepper

Phytopathology, 1989
Resistance to tobacco etch virus (TEV) was identified in pepper genotypes by comparing symptom severity; incubation period; viral antigen concentration, quantified by enzyme-linked immunosorbent assay (ELISA); and ability to become infected relative to the reaction of the susceptible Yolo Wonder B.
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Tobacco etch virus (tobacco etch)

PlantwisePlus Knowledge Bank, 2022
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Inheritance of Partial Resistance to Tobacco Etch Virus and Tobacco Vein Mottling Virus in Burley Tobacco Cultivar Sota 6505

Plant Disease, 1993
Tobacco etch virus (TEV) and tobacco vein mottling virus (TVMV) are serious diseases of burley tobacco and are controlled most effectively by use of resistant cultivars. The inheritance of resistance to TEV and TVMV in the burley tobacco cultivar Sota 6505 and allelism with other sources of potyvirus resistance were evaluated. Crosses were made between
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Resistance to tobacco etch virus in Lycopersicon hirsutum

1996
International ...
Légnani, R., Gebre Selassie, Kahsay, Marchoux, Georges, Laterrot, H.   +3 more
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PURIFICATION AND CHARACTERIZATION OF RECOMBINANT TOBACCO ETCH VIRUS PROTEASE

1996
The development of expression systems which permit the overproduction of large amounts of protein is a very important advancement in modern biotechnology. Recent developments in cloning have eased some of the traditional problems of obtaining high yields of pure product. New techniques enable the researcher to express and purify proteins of interest by
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Cylindrical Inclusions in the Cytoplasm of Leaf Cells Infected with Tobacco Etch Virus

Science, 1966
Combined tracings from electron micrographs of serial sections of leaf tissue infected with tobacco etch virus show that one type of cytoplasmic inclusion, when sectioned in different planes, can produce configurations which have been interpreted as being two distinct types of inclusion bodies.
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Tobacco etch virus cylindrical inclusions: Antigenically unrelated to the causal virus

Virology, 1969
J F, Shepard, T A, Shalla
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