Results 111 to 120 of about 64,179 (311)

Replication of tobacco mosaic virus RNA [PDF]

open access: yesPhilosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 1999
The replication of tobacco mosaic virus (TMV) RNA involves synthesis of a negative–strand RNA using the genomic positive–strand RNA as a template, followed by the synthesis of positive–strand RNA on the negative–strand RNA templates.
openaire   +3 more sources

High yield production of the antifungal proteins PeAfpA and PdAfpB by vacuole targeting in a TMV‐based expression vector

open access: yesPlant Biotechnology Journal, EarlyView.
Summary Antifungal proteins (AFPs) derived from filamentous fungi show great potential against economically significant fungi that cause plant diseases and consequently threat food safety and security. This study focuses on the Penicillium expansum PeAfpA and Penicillium digitatum PdAfpB proteins and their activity against several phytopathogens.
Adrià Bugeda   +6 more
wiley   +1 more source

Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco

open access: yesBMC Plant Biology, 2016
Background The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi.
Teppei Sugawara   +3 more
doaj   +1 more source

Expression of a constitutively active nitrate reductase increases SARS‐CoV‐2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence

open access: yesPlant Biotechnology Journal, EarlyView.
Summary The production of coronavirus disease 2019 vaccines can be achieved by transient expression of the Spike (S) protein of Severe Acute Respiratory Syndrome Coronavirus 2 in agroinfiltrated leaves of Nicotiana benthamiana, a process promoted by the co‐expression of viral silencing suppressor P19.
Louis‐Philippe Hamel   +9 more
wiley   +1 more source

High-density SNP genetic linkage map construction and quantitative trait locus mapping for resistance to cucumber mosaic virus in tobacco (Nicotiana tabacum L.)

open access: yesCrop Journal, 2019
Genetic linkage maps are essential for studies of genetics, genomic structure, and genomic evolution, and for mapping quantitative trait loci (QTL).
Lirui Cheng   +8 more
doaj   +1 more source

Seamless, modular binary vectors for plant cell and molecular biology

open access: yesPlant Biotechnology Journal, EarlyView.
Summary In plants, genetic manipulations are commonly performed using Agrobacterium tumefaciens, a plant pathogen whose abilities as a ‘natural genetic engineer’ have been co‐opted for biotechnological applications. In the widely used binary vector systems, Agrobacterium already contains a plasmid with most of the genes needed for virulence and is ...
Rory Greenhalgh   +5 more
wiley   +1 more source

Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus

open access: yesViruses, 2013
Alfalfa mosaic virus (AlMV) RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs) have been previously shown to be encapsidated into virions by coat protein (CP) expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 ...
Vidadi Yusibov   +6 more
doaj   +1 more source

Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe‐Only Nitrogenase in Plant Mitochondria

open access: yesPlant Biotechnology Journal, EarlyView.
Design of more soluble AnfH variants for expression in plant mitochondria. ABSTRACT Engineering nitrogenase directly into crops is a long‐held aspiration in plant biotechnology. Of the three types, the Fe‐only nitrogenase is a promising engineering target, as it has a simpler maturation pathway than the MoFe‐ and VFe‐nitrogenases and does not require ...
Christina M. Gregg   +8 more
wiley   +1 more source

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