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, 2019
Direct electron detectors are an essential asset for the resolution revolution in electron cryo microscopy of biological objects. The direct detectors provide two modes of data acquisition; the counting mode in which single electrons are counted, and the
B. Song +5 more
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Direct electron detectors are an essential asset for the resolution revolution in electron cryo microscopy of biological objects. The direct detectors provide two modes of data acquisition; the counting mode in which single electrons are counted, and the
B. Song +5 more
semanticscholar +1 more source
Dislocations in Tobacco Mosaic Virus
Nature, 1973WE would like to comment on the recent article with this title by Harris1 in which he reproves our group for an alleged incorrect usage of the term “dislocation”. In none of our papers do we write that the helical form of TMV is “dislocated or contains a dislocation”, but rather that a disk will have to undergo a dislocation to be transformed into a ...
A, Klug, P J, Butler
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Assembly of tobacco mosaic virus
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1976The assembly of tobacco mosaic virus requires the presence of a particular protein aggregate, the disk. During the nucleation, a specific region of the RNA interacts with a single disk, to bring about a necessarily cooperative transition from the paired two-layer structure to a short segment of nucleo-protein helix. There is a high selectivity for this
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Pseudovirions of tobacco mosaic virus
Virology, 1971Abstract Tobacco mosaic virus (TMV) preparations contain a small percentage of pseudovirions. These particles, which are thought to consist of host RNA encapsidated with TMV protein, can be separated from virus particles by electrophoresis in 0.5% agarose gel. Pseudovirion RNA is smaller than and has a broader size distribution than viral RNA.
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Proteins in tobacco mosaic virus-infected tobacco plants
Biochemical and Biophysical Research Communications, 1970Abstract Tobacco mosaic virus (TMV)-infected leaf tissue was exposed to 3 H-leucine following a treatment with actinomycin D. Uninfected leaf tissue was similarly treated, but was labeled with 14 C-leucine. Extracts of the two tissues were combined and analyzed by electrophoresis in sodium dodecyl sulfate (SDS)-containing polyacrylamide gels.
M, Zaitlin, V, Hariharasubramanian
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Aphid Transmission of Tobacco Mosaic Virus
Science, 1969Aphids ( Myzus persicae Sulz.) can acquire tobacco mosaic virus from tobacco leaves coated with a virus suspension and inoculate it into healthy leaves. Transmission depends on virus concentration, period of acquisition, previous feeding history of the aphids, and time between acquisition and transmission feedings ...
J S, Lojek, G B, Orlob
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Cation binding by tobacco mosaic virus
Virology, 1977Abstract Hydrogen ion titration curves of tobacco mosaic virus and its protein, alone or in the presence of various multivalent ions, have been measured. Three groups titrating near neutral pH in the virion have significant metal ion binding, but the tightest of these binding sites, significantly specific for Ca 2+ over Mg 2+ appears to be absent ...
A C, Durham, D A, Hendry
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ZONE ELECTROPHORESIS OF TOBACCO MOSAIC VIRUS
Canadian Journal of Biochemistry and Physiology, 1959An apparatus is described for the preparative zone electrophoresis of tobacco mosaic virus in agar gel. The isolated virus does not have any detectable ribonuclease activity.
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Fine Structure of Tobacco Mosaic Virus
Nature, 1956ALTHOUGH tobacco mosaic was one of the first viruses to be examined in the electron microscope, electron microscopy has failed to reveal any fine structure, even though it has been repeatedly looked for1,2, especially since the introduction3 of metal shadow casting in 1945.
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Growth of Tobacco Mosaic Virus Particles
Science, 1959Past studies have characterized the structure of tobacco mosaic virus particles by a variety of methods. In the present report the screw dislocation theory of crystal growth is applied to the formation of tobacco mosaic virus particles. The growth mechanism is shown to account for the rodlike morphology. It is also deduced that the biosynthetic process
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