Results 131 to 140 of about 9,278,986 (351)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Study of a tap failure at the internal threads machining

, 2019
The article aims to study a tap failure during the internal threads making. Within the research, the synergic effects of cutting speed and helix angle on the durability of selected taps have been investigated including detection of the types tool wear ...
Modrák, Vladimír, Monka, Peter Pavol, Hric, Slavomír, Pastucha, Peter, Monkova, Katarína   +4 more
core  

Impacts of soil physical and mechanical behaviors under different tillage depths for agrotechnical operation in Bukito, Sidama, Ethiopia

Scientific Reports
Tillage operation aims to create a favorable environment for seed germination of agricultural crop production practices. Physio-mechanical properties of soil directly affecting soil behaviors and determinants in initial conditions affecting soil failure.
Samuel Ketena, Girma Gebresenbet, Kishor Purushottam Kolhe, Miheret Dananto, Yared Seifu, Markos Mathwos   +5 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Failure Analysis of Injection wheel cleaning tool

, 2003
A set of three injection wheel cleaning tools containing one failed tool was sent to the laboratory for determining the mode of fracture. Examination revealed that the tool had failed under overload.
Sujata, M, Bhaskaran, TA, Bhaumik, SK
core  

Mechanical Load Identification for Spontaneous Tool Failure Monitoring

, 1996
The problem of spontaneous cutting tool failure, such as tool chipping and breakage, is becoming more and more important in the manufacturing technology. The sponataneous tool failure is usually associated with the stresses subjected on the tool during a
Zhou, Jinming,, Lund University.
core   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Failure mode and effects analysis applied to central venous catheter placement

BMJ Open Quality
Despite diligent efforts, complications continue to occur during the placement of central venous catheters (CVCs). Healthcare Failure Mode and Effect Analysis has been promoted as a process improvement tool and this review describes the strategic ...
Daniel Harwood, Bruno Maranhao, James R Duncan, Ellen Wertenberger, Jacob Grant, Mona Ostman   +5 more
doaj   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source